Gel de plaquetas: arcabouço 3D para cultura celular

Moroz, Andrei; Bittencourt, R.A.C.; Felisbino, Sérgio Luís; Pereira, Hamilton da Rosa; Rossi-Ferreira, Rosana; Deffune, Elenice

doi:10.1590/s1413-78522009000200008

Cited by 23 publications

(7 citation statements)

References 7 publications

(6 reference statements)

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“…These data indicated that α7β1 was sensitive to PRP and alignment and that α7 and β1D were not mediating MyoG and MyHC increases. Instead, these increases might be influenced by inherent ECM proteins in PRP like fibrinogen and fibronectin [37][38][39][40], or by the effect of PRP on ECM synthesis [4,41].…”

Section: Discussionmentioning

confidence: 99%

Platelet-rich plasma and alignment enhance myogenin via ERK mitogen activated protein kinase signaling

et al. 2018

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Volumetric muscle loss is debilitating and involves extensive rehabilitation. One approach to accelerate healing, rehabilitation, and muscle function is to repair damaged skeletal muscle using regenerative medicine strategies. In sports medicine and orthopedics, a common clinical approach is to treat minor to severe musculoskeletal injuries with platelet-rich plasma (PRP) injections. While these types of treatments have become commonplace, there are limited data demonstrating their effectiveness. The goal of this study was to determine the effect of PRP on myoblast gene expression and protein production when incorporated into a polymer fiber. To test this, we generated extracellular matrix mimicking scaffolds using aligned polydioxanone (PDO) fibers containing lyophilized PRP (SmartPReP 2, Harvest Technologies Corporation, Plymouth, MA). Scaffolds with PRP caused a dose-dependent increase in myogenin and myosin heavy chain but did not affect myogenic differentiation factor-1 (MyoD). Integrin α7β1D decreased and α5β1A did not change in response to PRP scaffolds. ERK inhibition decreased myogenin and increased Myod on the PDO-PRP scaffolds. Taken together, these data suggest that alignment and PRP produce a substrate-dependent, ERK-dependent, and dose-dependent effect on myogenic differentiation.

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Section: Discussionmentioning

confidence: 99%

Platelet-rich plasma and alignment enhance myogenin via ERK mitogen activated protein kinase signaling

et al. 2018

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“…Vários destes métodos biológicos encontram-se associados à Engenharia de Tecidos [62][63][64] . Como in vivo, os tecidos projetados in vitro devem fornecer transporte de nutrientes, estabilidade mecânica, coordenação de processos multicelulares e um microambiente celular que preserve a estabilidade fenotípica das células [65] . Dentre os hidrogéis aplicáveis à engenharia de tecido cartilaginoso, o AH é um GAGs presente na matriz extracelular do tecido cartilaginoso, que fornece um microambiente capaz de manter a homeostase e preservar o estado diferenciado dos tecidos.…”

Section: Aplicação De Hidrogéis a Base De áCido Hialurônico E Quitosaunclassified

Hidrogéis a base de ácido hialurônico e quitosana para engenharia de tecido cartilaginoso

Nascimento

Lombello

2016

Polímeros

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AesumoA Engenharia de Tecidos envolve o desenvolvimento de novos materiais ou dispositivos capazes de interações específicas com os tecidos biológicos, buscando a utilização de materiais biocompatíveis que devem servir como arcabouço para o crescimento de células in vitro, organizando e desenvolvendo o tecido que posteriormente será implantado no paciente. Uma variedade de arcabouços como hidrogéis poliméricos, sintéticos e naturais, têm sido investigados para a expansão de condrócitos in vitro, visando o reparo da cartilagem lesionada. Um hidrogel de interesse particular na regeneração de cartilagem é o ácido hialurónico (AH). Trata-se de um biopolímero atraente para a fabricação de arcabouços artificiais para Engenharia de Tecidos por ser biocompatível e biodegradável. A biocompatibilidade do AH deve-se ao fato de estar presente na matriz extracelular nativa, deste modo, cria-se um ambiente propício que facilita a adesão, proliferação e diferenciação celular, além da existência de sinalização celular específica, o que contribui para a regeneração do tecido. O uso de hidrogel composto de ácido hialurónico e quitosana (QUI) também tem sido investigado em aplicações de Engenharia de Tecidos de cartilagem, com resultados promissores. Baseando-se nestas informações, o objetivo este trabalho foi investigar as alternativas disponíveis para regeneração tecidual da cartilagem e conhecer mais detalhadamente as relações entre células e biomateriais. Palavras-chave: ácido hialurônico, biocompatibilidade, engenharia de tecidos, quitosana, cultura de células. AbstractTissue Engineering involves the development of new materials or devices capable of specific interactions with biological tissues, searching the use of biocompatible materials as scaffolds for cell growth in vitro, organizing and developing tissue that is subsequently implanted into the patient. A variety of scaffolds such as polymeric hydrogels, natural and synthetic, have been investigated for the expansion of chondrocytes in vitro in order to repair the damaged cartilage. A hydrogel of particular interest in cartilage regeneration is hyaluronic acid (HA). HA are attractive biopolymers for manufacturing artificial scaffolds for Tissue Engineering, it is biocompatible and biodegradable. The biocompatibility of HA is due to the fact that it is present in native extracellular matrix, thus creates an environment, which facilitates the adhesion, proliferation and differentiation, in addition to the existence of specific cell signaling, which contributes to tissue regeneration. The use of hydrogel composed of hyaluronic acid and chitosan (CHI) has also been investigated for applications in Tissue Engineering of soft tissues, like cartilage, with promising results. Based on this information, this study aims to investigate the alternatives available for cartilage tissue regeneration and meet more detail the relationships between cells and biomaterials.

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“…Sophisticated 3D cultures using scaffolds—or scaffolding—can be generated using several natural polymers (collagen, elastin, fibrin, chitosan, alginate, fibrinogen, and platelet-rich plasma), synthetic materials, which include PLLA (poly (L-lactic acid)), PGA (poly (glycolic acid)), elastomeric polyurethanes, decellularized tissues and organs, and others [ 18 , 19 , 20 , 21 , 22 , 23 ]. The main challenge in scaffold designing is to mimic the presence of the ECM, a network of fibrous proteins, primarily collagen, embedded in a highly hydrated gel of glycosaminoglycans, proteoglycans, and glycoproteins [ 11 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Alginate-Based 3D A549 Cell Culture Model to Study Paracoccidioides Infection

Santos

Oliveira

Fontes

et al. 2023

JoF

Self Cite

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A three-dimensional (3D) lung aggregate model based on sodium alginate scaffolds was developed to study the interactions between Paracoccidioides brasiliensis (Pb) and lung epithelial cells. The suitability of the 3D aggregate as an infection model was examined using cell viability (cytotoxicity), metabolic activity, and proliferation assays. Several studies exemplify the similarity between 3D cell cultures and living organisms, which can generate complementary data due to the greater complexity observed in these designed models, compared to 2D cell cultures. A 3D cell culture system of human A549 lung cell line plus sodium alginate was used to create the scaffolds that were infected with Pb18. Our results showed low cytotoxicity, evidence of increased cell density (indicative of cell proliferation), and the maintenance of cell viability for seven days. The confocal analysis revealed viable yeast within the 3D scaffold, as demonstrated in the solid BHI Agar medium cultivation. Moreover, when ECM proteins were added to the alginate scaffolds, the number of retrieved fungi was significantly higher. Our results highlight that this 3D model may be promising for in vitro studies of host–pathogen interactions.

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Gel de plaquetas: arcabouço 3D para cultura celular

Cited by 23 publications

References 7 publications

Platelet-rich plasma and alignment enhance myogenin via ERK mitogen activated protein kinase signaling

Platelet-rich plasma and alignment enhance myogenin via ERK mitogen activated protein kinase signaling

Hidrogéis a base de ácido hialurônico e quitosana para engenharia de tecido cartilaginoso

Alginate-Based 3D A549 Cell Culture Model to Study Paracoccidioides Infection

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