Improved assessment of wheat seeds vigor

Abstract

The tetrazolium test is widely used for seed vigor assessment of various plant species. For wheat, however, there is no methodology specifically recommended. This study aimed at determining an efficient procedure to evaluate vigor of wheat seeds by this test; besides allowing the establishment of seed quality classes. Thus, four different wheat seed lots were assessed by determining the moisture content and through tests of germination, accelerated aging, and field seedling emergence. The following methodologies were assessed: 1) longitudinal bisection of seed, with subsequent placement of the two halves on filter paper moistened with a 1.0% tetrazolium solution, at 30º C, for 2 h; 2) longitudinal bisection, with subsequent immersion of one seed half into a 1.0% tetrazolium solution, at 30º C, for 3 h; and 3) longitudinal bisection, with subsequent immersion of one seed half into a 0.075% tetrazolium solution, at 40º C, for 2 h. It was concluded that the tetrazolium test is more efficient in evaluating wheat seed vigor when performed with immersion of one half of the seed into a 0.075% tetrazolium solution (40º C, for 2 h) or a 0.1% tetrazolium solution (30º C, during 3 h); allowing to sort seeds into four quality classes. Index terms:Triticum aestivum, physiological quality, viability. RESUMOO teste de tetrazólio é amplamente utilizado para avaliar o vigor de sementes de várias espécies. Porém, para o trigo, não há recomendação de metodologia específica. O objetivo, neste estudo, foi determinar um procedimento eficiente para avaliar o vigor de sementes de trigo, por meio deste teste, permitindo estabelecer classes de qualidade. Foram avaliados quatro diferentes lotes de sementes de trigo, por meio da determinação do grau de umidade e dos testes de germinação, envelhecimento acelerado e emergência de plântulas em campo. Foram testadas as seguintes metodologias: 1) bissecção longitudinal, com disposição das duas metades sobre papel embebido em solução de tetrazólio a 1,0%, a 30° C, por 2 h; 2) bissecção longitudinal, com imersão de uma das metades em solução de tetrazólio a 0,1%, a 30° C, por 3 h; e 3) bissecção longitudinal, com imersão de uma das metades em solução de tetrazólio a 0,075%, a 40° C, por 2 h. Concluiu-se que o teste de tetrazólio é mais eficiente quando conduzido mediante imersão de uma das metades da semente em soluções de tetrazólio a 0,075% (40° C, por 2 h) ou a 0,1% (30° C, por 3 h), permitindo a classificação das sementes em quatro classes de qualidade. Termos para indexação:Triticum aestivum, qualidade fisiológica, viabilidade.

Alternative treatment to stimulate the germination of recently harvested wheat seeds

“…Journal of Seed Science, v.38, n.4, p. [344][345][346][347][348][349]2016 Tetrazolium test -viability in order to perform it, the methodology proposed by Carvalho et al (2012) was followed, cutting seeds in longitudinal bi-sections along the embryo and the endosperm and placing one seed half in 3 mL of 0.075% solution of 2,3,5-triphenyltetrazolium chloride, in a plastic cup (50 mL capacity), at 40 °C in the dark, for 2 hours. After that, seeds were individually evaluated and separated into feasible and non-feasible, as proposed by Carvalho et al (2012), to confirm wheat seed viability or not.…”

“…After that, seeds were individually evaluated and separated into feasible and non-feasible, as proposed by Carvalho et al (2012), to confirm wheat seed viability or not.…”

Invitro Regeneration and Mass-Clonal Proliferation of Wheat through embryonic formation

“…belongs to family gramineae/poaceae and it occupies 6 th place in the rank of most cultivated food crops, is growing all over the world and the most important cereal crop of Pakistan. Whilst, the production of wheat crop increased by the adoption of efficient cultural practices of crops such as use of best quality of seeds become necessary for cultivation of crop [4,5]. Although, tissue culture practices promising the production of high-quality types of seeds.There are many beneficial and useful wheat verities has been developed through controlled Invitro culture for drought tolerance The purpose of this study was toexamined the optimize concentration of MS medium supplemented with (BAP), (IAA) and to stabilize the method of regeneration of wheat on controlled laboratory condition.…”

Injúrias mecânicas, análise de imagens e potencial fisiológico de sementes de trigo

“…Também foram pesquisadas as perdas em função do tipo de colheita para soja (COSTA; MESQUITA; HENINNG, 1979;CAMPOS et al, 2005) e milho-pipoca (SOUZA et al, 1998 , 1975;BORBA et al, 1994BORBA et al, , 1996LERSCHI JUNIOR;STORCK, 2002) e sorgo (MARTINS NETTO et al, 1999); de solução de amaranto para milho (OLIVEIRA et al, 1998;BRANDAO JUNIOR et al, 1999;FESSEL et al, 2003) ou de iodo para milho (OLIVEIRA et al, 1998;BRANDAO JUNIOR et al, 1999;SILVA, 2003;MARCHI et al, 2006) e milho-doce (NASCIMENTO; PESSOA; BOITEUX, 1994). Porém, tais métodos, além de serem destrutivos, não permitem observar com precisão as injúrias diretamente relacionadas com as anormalidades nas plântulas ou com a morte dos embriões (CICERO et al, 1998;BANZATO JUNIOR, 2003), pois não identificam as injúrias internas na semente e não consideram a localização das injúrias externas (CICERO et al, 1998 (LEON et al, 1986;CICERO et al, 1998;CARVALHO et al, 1999;BANZATTO JUNIOR, 2003), milho doce (GOMES JUNIOR; CICERO, 2012), feijão (LEON et al, 1986;PINTO, 2008;MONDO et al, 2009) e soja (LEON et al, 1986;GIRARDIN, CHAVAGNAT, BOCKSTALLER, 1993;OBANDO FLOR et al, 2004;PINTO et al, 2009 VAN DAMME, 2003) e tetrazólio (CARVALHO et al, 2012 contudo com sucesso para tal avaliação (FRANCO et al, 2009;TUNES et al, 2011).…”