In rose (Rosa hybrida), flower senescence is accelerated by ethylene and delayed by cytokinins (CTKs). However, the effectors that regulate these processes are not currently understood. In this study, we identified an APETALA2/ethylene-responsive factor (AP2/ERF) gene, RhERF113, which was induced by ethylene and upregulated during flower senescence in most floral organs, including sepal, petal, stamen, and pistil. The virus-induced gene silencing (VIGS) of RhERF113 expression accelerated rose flower senescence, which was accompanied by a lower CTK content in the flowers. This accelerated senescence could be restored by exogenous CTK treatment. Moreover, the expression levels of genes related to CTK biosynthesis and signaling, including ISOPENTENYL TRANSFERASE 5 (RhIPT5), RhIPT8, HIS KINASE 2 (RhHK2), RhHK3, CYTOKININ RESPONSE REGULATOR 3 (RhCRR3), RhCRR5, RhCRR8, HOMEOBOX PROTEIN 6 (RhHB6), and PATHOGENESIS-RELATED 10.1 (RhPR10.1), were decreased in the RhERF113-silenced rose flowers. Taken together, our results demonstrate that RhERF113 delays ethylene-induced flower senescence by increasing the CTK content of the floral tissues.
Current study was carried in order to understand the significance of feeding practices for small ruminants. In this regards a detailed review of already conducted researches was performance, whereby obtained facts were found to be much interesting and valuable. It was indicated that the profitable small ruminants farming depends upon feeding practices because the feed cost about 70% in farming. Small ruminants are often offered grazing on summer and winter fodders for maintenance and production requirements. However, commercial production demands a change in feeding practice with a trend for more efficient utilization of scarce feed resources. It was further stated that goats spent more time on eating than sheep, while ruminating time remain higher in sheep than goats. Drinking time appears different among these species. Goats spent more time on playing and resting than sheep. Moreover, in extensive, semi-intensive and intensive feeding systems, dry matter intake was noticed higher in sheep than goats. Crude protein intake was reported significantly higher in sheep than goats fed intensively. NDF and ADF intake was higher in goats than sheep. Average daily weight gain was higher in sheep than goats on the extensive system followed by the semi-intensive system. Feed efficiency was similar in both species, while the cost of gain per kg was more economical in goats compared sheep. In conclusion both species performed better on extensive feeding system than the other systems. It might be because of natural grazing behavior.
Fusarium nivale (Fr.) Ces. a recently reported fungus of mango malformation disease (MMD)is a predominant and virulent fungus in mango orchards of Sindh, Pakistan. In the current study in vitro and in vivo attempts were made with commercial fungicides to reduce the severity of F. nivale. Mycelial growth of F. nivale was significantly inhibited at low and high doses of Thiophanate methyl and Fosetyl- Aluminium. Metalaxyl+Mancozeb and Mancozeb also reduced growth of fungus at their high doses (6.830 and 11.900mm), respectively, as compared to Copper oxychloride (18.083 mm) and control (40.750 mm). Thiophanate methyl and Fosetyl- Aluminium significantly reduced infection in Desi, Almas and Dusheri to 16.60 and 19.00%; 17.60 and 19.80%; and 20.60 and 22.00% after first spray, with decreased percent of malformation 72.33 and 68.33%; 71.11 and 67.54%; and 67.81 and 65.62% and over untreated control. The second spray of Thiophanate methyl and Fosetyl- Aluminium fungicides completely inhibited infection of F. nivale, and 100.0% reduction in malformation disease in Desi, Almas, and Dusheri as compared to Metalaxyl+Mancozeb (78.73, 73.84, 72.64%) and Mancozeb (73.65, 73.69, 69.41%), Copper oxychloride and in control. The application of Thiophanate methyl and Fosetyl- Aluminium would be useful in integrated management of MMD.
The auxin IAA is a vital plant hormone in controlling growth and development, but our knowledge about its complicated biosynthetic pathways and molecular regulation are still limited and fragmentary. cytokinin induced root waving 2 (ckrw2) was isolated as one of the auxin-deficient mutants in a large-scale forward genetic screen aiming to find more genes functioning in auxin homeostasis and/or its regulation. Here we show that CKRW2 is identical to Histone Monoubiquitination 1 (HUB1), a gene encoding an E3 ligase required for histone H2B monoubiquitination (H2Bub1) in Arabidopsis. In addition to pleiotropic defects in growth and development, loss of CKRW2/HUB1 function also led to typical auxin-deficient phenotypes in roots, which was associated with significantly lower expression levels of several functional auxin synthetic genes, namely TRP2/TSB1, WEI7/ASB1, YUC7 and AMI1. Corresponding defects in H2Bub1 were detected in the coding regions of these genes by chromatin immunoprecipitation (ChIP) analysis, indicating the involvement of H2Bub1 in regulating auxin biosynthesis. Importantly, application of exogenous cytokinin (CK) could stimulate CKRW2/HUB1 expression, providing an epigenetic avenue for CK to regulate the auxin homeostasis. Our results reveal a previously unknown mechanism for regulating auxin biosynthesis via HUB1/2-mediated H2Bub1 at the chromatin level.
Gram pod borer, Helicoverpa armigera Hubner is known to be a major constraint of chickpea production which causes serious economic loess. The management of this pest in any crop is always been challenge to the growers, famers and researcher. Thus, present study evaluated some promising entomopathogenic fungi for the sustainable management of H. armigera to minimize the economic loss in chickpea. Five different fungal isolates viz; Beauveria bassiana, Trichoderma virens, Trichoderma hamatum, Trichoderma koningii, and Paecilomyces sp. were used as entomopathogenic against gram pod borer, through dipping and poison food methods under laboratory conditions. The entomopathogenic potential of different fungal strains revealed significantly (P<0.05 = 0.0000) highest mortality with B. bassiana (46.67%) follo wed by T. koningi (23.33%), T. virens (11.11%) and T. hamatum (8.33%) through dipping method. In case o f poison food method significantly highest mortality was recorded with T. koningi (20%) followed by B. bassiana (6.66%) after 24 h. The mortality with B. bassiana after 96 h was become higher (41.667%) compared with other strains. No mortality was recorded with Paecilomyces sp. and control (dipped in simple water) in both methods. It is obvious that microbial control agents are very effective and the promising entomopathogenic fungi of current study are hoped would be helpful for eco-friendly and alternative to chemical pesticides for sustainable management of H. armigera in chickpea.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.