Antibacterial activity of propolis produced by Frieseomelitta varia

Abstract

To contribute to the development of antibacterial products from propolis produced by native Brazilian bees, twenty-nine samples of propolis collected from hives in the state of Minas Gerais, Brazil, were screened for in vitro activity against Aeromonas hydrophila, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. Among the samples from native Brazilian bees, only that from Frieseomelitta varia (Lepeletier, 1836) inhibited in vitro bacterial growth. Consequently, this propolis underwent fractionation by chromatographic methods monitored through Agar-diffusion assays with these bacteria, which resulted in the isolation and identification of 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C), which showed MIC of 62.5 and 250 µg/mL against B. subtilis and S. aureus, respectively. This result indicates the potential of F. varia to produce therapeutic propolis.Index terms: Artepillin C, stingless bees, antibiotic. RESUMOPara contribuir para o desenvolvimento de produtos antibacterianos obtidos de própolis produzidos por abelhas nativas do Brasil, 29 tipos de própolis coletados de diferentes colméias no Estado de Minas Gerais, Brasil, foram avaliados quanto à atividade in vitro contra Aeromonas hydrophila, Bacillus subtilis, Pseudomonas aeruginosa, e Staphylococcus aureus. Dentre as amostras de abelhas nativas, somente a de Frieseomelitta varia (Lepeletier, 1836) inibiu, em teste in vitro, o crescimento bacteriano. Consequentemente, essa própolis foi submetida a métodos cromatográficos para o fracionamento biomonitorado por teste de difusão em Agar com as referidas bactérias, o que resultou no isolamento e identificação do ácido 3,5-diprenil-4-hidroxicinâmico (artepelin C), que apresentou CIM de 62,5 e 250 µg/mL frente a B. subtilis e S. aureus, respectivamente. Esses resultados indicam o potencial de F. varia para a produção de própolis terapêutica.Termo para indexação: Artepelin C, abelhas sem ferrão, antibiótico.

Hydroalcoholic extract from Baccharis dracunculifolia recovers the gastric ulcerated tissue, and p‐coumaric acid is a pivotal bioactive compound to this action

“…The structures of the isolated compounds (Fig. ) were identified on the basis of 1 H NMR and 13 C NMR spectroscopic data (Supporting Information), as well as high‐resolution electrospray ionization mass spectrometer (MS (ESI‐MS)) (Supporting Information), in comparison with data published in the literature .…”

Metabolomics Approach Expands the Classification of Propolis Samples from Midwest Brazil

“…Compound 35 was obtained as an oil, and its molecular formula was established as C 19 H 24 O 4 on the basis of its HRESIMS ( m / z 315.1601 [M – H] − ) data. Comparison of the UV and 1 H and 13 C NMR data of 35 (Table ) with those of artepillin C ( 40 ) indicated that their structures only differed in the nature of one of the two prenyl moieties; thus, 35 comprised a hydroxydiprenylcoumaric acid derivative. Accordingly, a pair of broad singlets at δ H 4.88 and 4.99 was ascribed to the methylene hydrogens of a vinyl group, wherein the respective carbon resonances were observed at δ C 111.5 and 145.8.…”

“…36 The NMR data of 24 were thus an invaluable tool for allowing its correct identification as a 3-methoxyflavone derivative and found to be in agreement with those of 3-O-methylkaempferol, also known as isokaempferide, 41 and reported in other propolis samples. 42 Accordingly, the 1 H and 13 C NMR data of 3,4-dihydroxybenzoic acid (2), caffeic and coumaric acids (4 and 8), the dihydroflavonols 12 and 19, the flavonols 20 and 34, and the prenylated phenylpropanoids 28, 30, 31, 32, 40, 42, and 43 (Supporting Information) confirmed their identity, which was 2) with those of artepillin C (40) 43 indicated that their structures only differed in the nature of one of the two prenyl moieties; thus, 35 comprised a hydroxydiprenylcoumaric acid derivative. Accordingly, a pair of broad singlets at δ H 4.88 and 4.99 was ascribed to the methylene hydrogens of a vinyl group, wherein the respective carbon resonances were observed at δ C 111.5 and 145.8.…”

“…The 1 H and 13 C NMR data of the other isolated compounds ( 2 , 4 , 8 , 12 , 19 – 21 , 23 , 24 , 28 , 30 – 32 , 34 , 40 , 42 , and 43 ) are in accordance with the literature ,,,,− and are available in the Supporting Information.…”