In vitro induction of callus from cotyledon and hypocotyl explants of Glycine wightii (Wight &amp; Arn.) Verdc.

Silva, André Luis Coelho da; Caruso, Cecília Sulzbacher; Moreira, Renato de Azevedo; Horta, Ana Cecília Góes

doi:10.1590/s1413-70542003000600011

Cited by 5 publications

(4 citation statements)

References 7 publications

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“…It was reported that leaf explants of Catha ran thus roseus cultured on MS medium having 4.06 µM 2,4D in combination with 2.3 µM Kn could produce good fri able, high biomass callus (Garg 2010). The present study is in harmony with the works of Da Silva et al (2003) and Garg (2010), who used same combination of the 2 plant growth regulators (2,4D + Kn). 2,4D in combination with Kn could induced callus formation in many plant species; Tylophora indica (Sadguna et al, 2013) and Rauvolfia serpentina (Gupta et al, 2014).…”

Section: Discussionsupporting

confidence: 79%

“…Da Silva et al (2003) noted that 83.2 % and 86.4 % callus formation from the cotyledon and hypocotyl ex plants of Glycine wightii, when they were cultured on MS medium fortified with 1.0 mg/l 2,4D in combination with 0.10 mg/l Kn. It was reported that leaf explants of Catha ran thus roseus cultured on MS medium having 4.06 µM 2,4D in combination with 2.3 µM Kn could produce good fri able, high biomass callus (Garg 2010).…”

Section: Discussionmentioning

confidence: 99%

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Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant

Subbaiyan¹,

Thangapandian²

2017

Bot Pac

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An efficient protocol of callus induction, shoot multiplication and plant rege ne ra tion from cotyledon and leaf explants of Ceropegia bulbosa has been elaborated. The highest percentage (90.66) of callus induction was observed when cotyle do nary explants cultured on MS medium having 0.5 mg/L 2,4D in combination with 0.05 mg/L Kn followed by 86.25 % callus induction from leaf explant at 0.5 mg/l BAP in combination with 0.05 mg/l Kn. Shoot multiplication was ob served, cotyledon derived callus cultured on 1.0 mg/L BAP in combination with 0.10 mg/L NAA could induce number of shoots (4.71) and their lengths (6.13 cm). Cotyledonary callus derived microshoots gave the highest rooting percen tage (92.26 %) root numbers (13.14) and their lengths (8.87 cm) were observed on halfstrength MS medium fortified with 0.4 mg/L IBA. In vitro derived plant lets were hardened in the greenhouse and successfully established into the soil. Переведено редакцией.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant

Subbaiyan¹,

Thangapandian²

2017

Bot Pac

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“…The presence of hormone 2,4-D in this study is essentially important hormone to induce callus from the explants even though the hormone kinetin was absent. The 2,4-D and kinetin hormone combinations have been reported greatly induce callus in plants like Arabidopsis thaliana [12], five citrus rootstock species [13], Nigella sativa [14] and Glycine wightii [15].…”

Section: Resultsmentioning

confidence: 99%

Callus Formation on Endosperm from Immature and Mature Fruits of Barringtonia Racemosa

Soder¹,

Khalid²,

Saleh³

et al. 2019

IJET

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Barringtonia racemosa is mangroves type of plant which had been extensively utilized in conventional practices for relieving ailments of pain and inflammation. Many studies have been done on ethnobotanical profiles, pharmacological activities and chemical compounds in Barringtonia racemosa. However, there is a limited study on callogenesis of this plant particularly from different maturity stage of fruits. The present study is to identify the callogenesis of Barringtonia racemosa from endosperm explants of immature and mature fruits in MS medium supplemented with different concentrations of hormones 2,4-Dichlorophenoxyacetic acid (2,4-D) (0, 0.5, 1.0, 1.5 and 2.0 mg/L) and Kinetin (KIN) (0, 0.5, 1.0, 1.5 and 2.0 mg/L). The optimum hormone combination was found in callus grown on endosperm of immature fruits in MS medium supplemented with 1.5 mg/L 2,4-D and 1.0 mg/L KIN. It was also found that the callus in this treatment grew profusely with highest fresh weight (0.513 ± 0.022 g), 100% callus induction and friable callus texture. The callus fresh weight on endosperm explants was higher in immature fruits compared to mature fruits for all the hormone combinations. Therefore, callogenesis were found more efficient from endosperm explant of immature fruits in Barringtonia racemosa species.

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“…Cultures were incubated under dark condition at a temperature of 25 ± 1 O C. One month after treatment, the percentage of embryogenic callus formation was observed. The frequency of somatic embryos formation can be influenced by genotype and composition of culture medium (Da Silva et al, 2003).…”

Section: Methodsmentioning

confidence: 99%

The effect of 2,4 dichlorophenoxyacetic acid on in vitro callogenesis of cocoa (Theobroma cacao L.)

Pancaningtyas

2015

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Cocoa (Theobroma cacao L.) development using modern breeding techniques can be facilitated by propagation of planting material through somatic embryogenesis. Various factors that may affect embryogenesis are the composition of culture medium and culture condition. Hormone commonly used to initiate the formation of callus is auxin with type 2.4-D (2.4 Dichlorophenoxy acetic acid). The aim of this study was to determine the effect of the addition of 2.4 -D hormoneson the process of cocoa embryogenesis. The treatments were arragged in factorial combination in completely randomized design, which consisted of two factors. Thefirst factor was the concentration of auxin 2,4-D 25 %, 50 %, 75 %, and 100 %; and the second factor was cocoa clones; Sulawesi 01 and Sulawesi 02. The resultshowed that the addition of 2.4-D hormone up to 100% on somatic embryogenesis of cocoa for Sulawesi 01 clone was not significantly different from Sulawesi 02 clone for all parameters. While on the addition of 2.4-D, there was significant difference between Sulawesi 01 and 02. Cocoa embryogenic callus using the addition of 2.4-D (25%-100%) was significantly different from control. Increased concentrations of 2,4-D hormone which is applied onto media would inhibit the formation of the somatic embryo. Addition of 2.4 D 25%, encouraged towards non-embryogenic callus. Keywords: 2.4 Dichlorophenoxy acetic acid, embryogenic callus, somatic embryos, cocoa, medium culture, hormone

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In vitro induction of callus from cotyledon and hypocotyl explants of Glycine wightii (Wight & Arn.) Verdc.

Cited by 5 publications

References 7 publications

Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant

Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant

Callus Formation on Endosperm from Immature and Mature Fruits of Barringtonia Racemosa

The effect of 2,4 dichlorophenoxyacetic acid on in vitro callogenesis of cocoa (Theobroma cacao L.)

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