Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant

Abstract

An efficient protocol of callus induction, shoot multiplication and plant rege ne ra tion from cotyledon and leaf explants of Ceropegia bulbosa has been elaborated. The highest percentage (90.66) of callus induction was observed when cotyle do nary explants cultured on MS medium having 0.5 mg/L 2,4D in combination with 0.05 mg/L Kn followed by 86.25 % callus induction from leaf explant at 0.5 mg/l BAP in combination with 0.05 mg/l Kn. Shoot multiplication was ob served, cotyledon derived callus cultured on 1.0 mg/L BAP in combination with 0.10 mg/L NAA could induce number of shoots (4.71) and their lengths (6.13 cm). Cotyledonary callus derived microshoots gave the highest rooting percen tage (92.26 %) root numbers (13.14) and their lengths (8.87 cm) were observed on halfstrength MS medium fortified with 0.4 mg/L IBA. In vitro derived plant lets were hardened in the greenhouse and successfully established into the soil. Переведено редакцией.