Abstract:Cocoa (Theobroma cacao L.) development using modern breeding techniques can be facilitated by propagation of planting material through somatic embryogenesis. Various factors that may affect embryogenesis are the composition of culture medium and culture condition. Hormone commonly used to initiate the formation of callus is auxin with type 2.4-D (2.4 Dichlorophenoxy acetic acid). The aim of this study was to determine the effect of the addition of 2.4 -D hormoneson the process of cocoa embryogenesis. The treat… Show more
“…The concentration of auxin present in the tissue stimulates the formation of embryogenic callus [15] and callus proliferation [16,17,18,19]. Auxin can activate signal transduction, allowing cells to undergo reprogramming and induce cell division towards callus growth or embryogenesis.…”
Garlic is a national priority commodity with regards to reducing imports. One of the obstacles in its national production is the limitation of seed quality. This study aimed to investigate how auxin impacts the regeneration of embryogenic callus in three local garlic varieties. The experiment employed a two-factor Complete Randomized Design, involving varieties (Lumbu Putih, Geol, and Sangga Sembalun) and auxin types: A (IAA 1 ppm), B (IAA 3 ppm), C (IAA 5 ppm), D (IBA 1 ppm), E (IBA 3 ppm), and F (IBA 5 ppm). Each treatment was repeated three times so that the total treatment was 54 experimental units. Embryogenic calli, weighing about 0.2 g, were cultivated on the treatment medium. The observed variables were the diameter and texture of the callus, as well as the number of green spots on the callus. The results showed no significant interaction between variety and auxin treatment, but variety had a significant effect on callus diameter. The largest callus diameter was shown by the Sangga Sembalun variety under treatment C (2.75 mm) with a friable texture, yellowish color, and rooted structure, while the smallest callus diameter was produced by the Geol variety in treatment E (1.20 mm) with a non-friable texture and white color. The highest number of green spots was also produced from Sangga Sembalun in treatment D (7 green spots). It was concluded that auxin plays a role in the regeneration of somatic embryos in garlic, but its effectiveness depends on the genotype.
“…The concentration of auxin present in the tissue stimulates the formation of embryogenic callus [15] and callus proliferation [16,17,18,19]. Auxin can activate signal transduction, allowing cells to undergo reprogramming and induce cell division towards callus growth or embryogenesis.…”
Garlic is a national priority commodity with regards to reducing imports. One of the obstacles in its national production is the limitation of seed quality. This study aimed to investigate how auxin impacts the regeneration of embryogenic callus in three local garlic varieties. The experiment employed a two-factor Complete Randomized Design, involving varieties (Lumbu Putih, Geol, and Sangga Sembalun) and auxin types: A (IAA 1 ppm), B (IAA 3 ppm), C (IAA 5 ppm), D (IBA 1 ppm), E (IBA 3 ppm), and F (IBA 5 ppm). Each treatment was repeated three times so that the total treatment was 54 experimental units. Embryogenic calli, weighing about 0.2 g, were cultivated on the treatment medium. The observed variables were the diameter and texture of the callus, as well as the number of green spots on the callus. The results showed no significant interaction between variety and auxin treatment, but variety had a significant effect on callus diameter. The largest callus diameter was shown by the Sangga Sembalun variety under treatment C (2.75 mm) with a friable texture, yellowish color, and rooted structure, while the smallest callus diameter was produced by the Geol variety in treatment E (1.20 mm) with a non-friable texture and white color. The highest number of green spots was also produced from Sangga Sembalun in treatment D (7 green spots). It was concluded that auxin plays a role in the regeneration of somatic embryos in garlic, but its effectiveness depends on the genotype.
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