BAP e substratos na aclimatização de plântulas de gloxínia (Sinningia speciosa Lood. Hiern.) provenientes de cultura de tecidos

Silva, Adriano Bortolotti da; Pasqual, Moacir; Maciel, Anna Lygia de Rezende; Dutra, Leonardo Ferreira

doi:10.1590/s1413-70542003000200002

Cited by 6 publications

(7 citation statements)

References 6 publications

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“…The acclimatized seedlings that survived had at least one leaf in good condition, which contributed toward the emission of roots; preexisting roots, originated during the in vitro rooting, were not functional, without the emission of secondary roots, and presented root tissue necrosis. Vermiculite showed a low capacity of aggregation with the roots, which negatively affected plant development, in agreement with Hoffmann et al (2001), who worked with apple tree rootstocks, and Silva et al (2003), with gloxinia plantlets.…”

Section: Resultssupporting

confidence: 88%

“…During in vitro growth, plants develop under controlled conditions, including enclosed environments, without gaseous exchanges, with high moisture in the air, low light intensity, and the use of sugars from the medium as a source of carbon and energy (Preece & Sutter, 1991;Sciutti & Morini, 1993;Pospísilová et al, 1999). Thus, the transplanting of in vitro-grown plantlets and the complete establishment in the greenhouse can be complex for some species (Van Huylenbroeck & Debergh, 1996;Ross-Karsten et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…The low effectiveness of vermiculite as a substrate in the acclimatization of apple tree rootstocks and gloxinia plantlets demonstrates the importance of this parameter in this process (Hoffmann et al, 2001;Silva et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Acclimatization of micropropagated Heliconia bihai (Heliconiaceae) plants

Rodrigues

Lima

Ambrosano

et al. 2005

Sci. agric. (Piracicaba, Braz.)

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To evaluate the behavior of micropropagated heliconia seedlings during the acclimatization process using different substrates and shade levels, seedlings of H. bihai (Lobster Claw I) were planted in plastic trays using the substrates: washed sand, vermiculite (medium texture), and PlantMax ® Horticultura, and shade cloths with percentages of 0% (full sun), 30%, 40%, 50%, 60%, 70%, and 80% of shade in relation to full sun. Best results were obtained for washed sand and PlantMax substrates at shade conditions starting from 50%, especially at 70% and 80%. Pre-existing roots from in vitro rootings were not functional, not presenting secondary roots, and showing root tissue necrosis. Acclimatized seedlings developed new roots. This finding suggests that this plant can be acclimatized without going through a laboratory rooting stage. Key words: Heliconia, ex vitro culture, rooting ACLIMATIZAÇÃO DE MUDAS MICROPROPAGADAS DE Heliconia bihai (HELICONIACEAE)RESUMO: A cultura da helicônia é a que apresenta maior crescimento entre o cultivo de flores tropicais para a exportação. Avaliou-se o comportamento de mudas de helicônias micropropagadas no processo de aclimatização, em diferentes substratos e níveis de sombreamento. Foram plantadas mudas de H. bihai (Lobster Claw I) em bandejas plásticas contendo os diferentes substratos: areia lavada, vermiculita (textura média) e PlantMax ® Horticultura. Na avaliação de níveis de sombreamento foi utilizado o telado (sombrite), com ordem de sombreamento 0% (pleno sol), 30%, 40%, 50%, 60%, 70% e 80%. O desempenho foi superior nos substratos areia lavada e PlantMax ® Horticultura e nas condições de sombreamento a partir de 50 %, em especial 70% e 80%. As raízes pré-existentes, oriundas do enraizamento in vitro, mostraram-se não funcionais, sem a emissão de raízes secundárias e apresentando necrose do tecido radicular. As mudas aclimatizadas desenvolveram novas raízes, o que sugere que esta cultura pode ser aclimatizada sem que as plântulas passem pela fase de enraizamento em laboratório. Palavras-chave: Helicônia, cultura ex vitro, enraizamento

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Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Acclimatization of micropropagated Heliconia bihai (Heliconiaceae) plants

Rodrigues

Lima

Ambrosano

et al. 2005

Sci. agric. (Piracicaba, Braz.)

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“…The acclimatization is characterized as a gradual adaptation of micropropagated plants in a greenhouse before transferring them to the field, aiming at minimizing the effects of a sudden change of environments, as they had been kept in a totally controlled and aseptic place (POSPÍSILOVÁ et al, 2007). This acclimatization consists of two stages: rooting (in vitro and ex vitro) and transfer to nonsterile conditions with controlled temperature and humidity (SILVA et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

n vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil

Menezes¹,

Blank²,

Costa³

et al. 2019

Biosci. J.

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Orchids are known for the beauty, exuberance, and color of their flowers and therefore represent one of the most coveted ornamental plants. Due to their high commercial value, several illegal practices of removal and commercialization have led to the verge of extinction. This study aimed to develop protocols for in vitro conservation under slow growth and acclimatization of species of the subfamily Epidendroideae (Orchidaceae), from the state of Sergipe, Brazil. Two experiments were conducted: the first one tested four concentrations of MS (MURASHIGE and SKOOG, 1962) (25%, 50%, 75%, 100%), three orchid species (Catasetum macrocarpum, Oeceoclades maculata, and Polystachya estrellensis), and two temperatures (18 and 25ºC); the second one tested three combinations of carbon sources and osmotic regulators (20 g L -1 sucrose; 10 g L -1 sucrose + 5 g L -1 de mannitol; 10 g L -1 sucrose + 5 g L -1 sorbitol) and the same three species and two temperatures. For the in vitro conservation of C. macrocarpum, O. maculata, and P. estrellensis over 450 days, the use of 25% of MS salts or 20 g.L -1 sucrose at 25ºC is recommended. C. macrocarpum seedlings were acclimatized using sand + shredded pine bark + worm castings at a ratio of 2:2:1 (w:w:w). The use of sand alone is recommended for the acclimatization of O. maculata.

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“…Entre os materiais mais utilizados, a vermiculita vem apresentando destaque tanto isolada como em misturas (LOPES et al, 2003;TOMBOLATO et al, 1987); entretanto, outros substratos, como areia, casca de coco, casca de arroz carbonizada, plantmax®, solo, turfa, podem ser empregados desde que apresentem caracterís-ticas químicas e físicas favoráveis (COURTIER e CLARKE, 1997;RORIZ, 1994;SCHMITZ et al, 2002;SILVA et al, 2003;SOUZA, 2000).…”

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Enraizamento de estacas foliares de violeta-africana (Saintpaulia ionantha Wendl.) em diferentes substratos

et al. 2005

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RESUMOO presente trabalho foi conduzido com objetivo de avaliar diferentes substratos no enraizamento de estacas foliares de violeta-africana (Saintpaulia ionantha Wendl). O trabalho foi desenvolvido em casa-de-vegetação, com tela de poliolefina 70%, no Centro de Ciências Agrárias da Universidade Federal do Espírito Santo, Alegre-ES. Utilizaram-se plantas adultas das cultivares New Mexico (flores lilás); Nancy (flores rosas); Stella (flores azuis) e Mike (flores brancas), que foram aclimatadas; posteriormente, retiraram-se propágulos compostos por uma folha com pecíolo, reduzida a 2/3 do tamanho original, os quais foram esterilizados em solução de hipoclorito de sódio à concentração de 4%, durante três minutos. Foram utilizados cinco substratos: terra+areia+esterco, areia, pó de xaxim, vermiculita e água destilada, colocados em potes plásticos de 200 mL. O delineamento experimental empregado foi o de blocos ao acaso, com quatro repetições de 10 pecíolos cada. Pelos resultados evidenciou-se que a cultivar Nancy apresentou 100% de enraizamento em todos os substratos após 40 dias. A maior porcentagem de enraizamento foi obtida em estacas mantidas na água e o melhor desenvolvimento inicial no substrato de pó de xaxim. Termos para indexação: Saintpaulia ionantha, estaquia, propagação. ABSTRACTThe present study was accomplished with the objective of evaluate different substrates on the rooting of african violet (Saintpaulia ionantha Wendl). The work was carried out in the greenhouse under a shading net (poliolefinas 70%) in the Agrarian Science Center of Universidade Federal do Espírito Santo, in Alegre-ES. Were utilized acclimated plants of african violet (Saintpaulia ionantha Wendl), cultivar New Mexico (lilac flowers); Nancy (pink flowers); Stella (blue flowers) e Mike (white flowers). The leaf stalks were reduced to 2/3 of the original size, sterilized with a 4% sodium hypochloride during three minutes. The substrates utilized were soil+sand+manure, sand, fern fiber dust, vermiculite and water in plastic pot (200 mL). The experimental design was carried out in blocks randomized, with four replications of 10 leaf stalks. The results indicated that cultivar Nancy presented 100% of rooting in all substrate after 40 days. The greater percentage of rooting was verificated in water and better initial development in fern fiber dust.

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BAP e substratos na aclimatização de plântulas de gloxínia (Sinningia speciosa Lood. Hiern.) provenientes de cultura de tecidos

Cited by 6 publications

References 6 publications

Acclimatization of micropropagated Heliconia bihai (Heliconiaceae) plants

Acclimatization of micropropagated Heliconia bihai (Heliconiaceae) plants

n vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil

Enraizamento de estacas foliares de violeta-africana (Saintpaulia ionantha Wendl.) em diferentes substratos

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