Análise morfométrica do colágeno dérmico a partir da segmentação por conglomerados (clusters) de cor

Miot, Hélio Amante; Brianezi, Gabrielli

doi:10.1590/s0365-05962010000300010

Cited by 26 publications

(17 citation statements)

References 9 publications

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“…The processing and image analysis were performed with the public domain software ImageJ 1.36 version (National Institutes of Health, Bethesda, USA; http://rsbweb.nih.gov/ij/) using plugin color deconvolution and the values of the amount of collagen as described in previous studies [12–14]. …”

Section: 0 Materials and Methodsmentioning

confidence: 99%

Low-level laser therapy (904nm) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin

Tatmatsu-Rocha

Ferraresi

Hamblin

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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Background and Objective Over the last decade we have seen an increased interest in the use of Low-Level Laser Therapy (LLLT) in diseases that involve increased oxidative stress. It is well established that hyperglycemia in diabetes elicits a rise in reactive oxygen species (ROS) production but the effect of LLLT remains unclear. This study aimed to investigate whether LLLT was able to improve oxidative/nitrosative stress parameters in the wound healing process in diabetic mice. Study Design/Materials and Methods Twenty male mice were divided into four groups: non-irradiated control (NIC), irradiated control (IC), non-irradiated and diabetic (NID), irradiated and diabetic (ID). Diabetes was induced by administration of streptozotocin. Wounds were created 120 days after the induction of diabetes in groups IC and ID and these groups were irradiated daily for 5 days (superpulsed 904 nm laser, average power 40 mW, 60 sec). All animals were sacrificed 1 day after the last irradiation and histology, collagen amount, catalase activity, nitrite and thiobarbituric acid reactive substances (TBARS) were measured. Results Histology showed that collagen fibers were more organized in IC and ID when compared to NID group, and significant differences in collagen content were found in group ID versus NID. Catalase activity was higher in IC group compared to other groups (p < 0.001). TBARS levels were higher in IC versus NIC, but were lower in ID versus NID (p < 0.001). Nitrite was lower in both irradiated groups versus the respective non-irradiated groups (p < 0.001). Conclusions Delayed wound healing in diabetes is still a challenge in clinical practice with high social costs. The increased production of collagen and decreased oxidative and nitrosative stress suggests that LLLT may be a viable therapeutic alternative in diabetic wound healing.

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Section: 0 Materials and Methodsmentioning

confidence: 99%

Low-level laser therapy (904nm) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin

Tatmatsu-Rocha

Ferraresi

Hamblin

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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“…The slides were examined under light microscope, five images per field (0.0018 mm 2 area) were captured with a digital camera (total magnification 400x) attached to the microscope (Olympus BX-49 light microscope and camera). The images were stored and subjected to counting of inflammatory cells, fibroblast cells, number of blood vessels, and evaluation of collagen density of all lesions with the aid of digital marking [18, 19]. …”

Section: Methodsmentioning

confidence: 99%

Effect of a Semisolid Formulation ofLinum usitatissimumL. (Linseed) Oil on the Repair of Skin Wounds

Franco

Aquino

Medeiros

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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The purpose of this study was to investigate the effects of a semisolid formulation of linseed oil, SSFLO (1%, 5%, or 10%) or in natura linseed oil on skin wounds of rats. We used wound models, incisional and excisional, to evaluate, respectively, the contraction/reepithelialization of the wound and resistance to mechanical traction. The groups (n = 6) treated with SSFLO (1% or 5%) began the process of reepithelialization, to a significant extent (P < .05), on the sixth day, when compared to the petroleum jelly control group. On 14th day for the groups treated with SSFLO (1% or 5%), 100% reepithelialization was found, while in the petroleum jelly control group, this was only 33.33%. Our study showed that topical administration of SSFLO (1% or 5%) in excisional wounds allowed reepithelialization in 100% of treated animals. Therefore, a therapeutic potential of linseed oil, when used at low concentrations in the solid pharmaceutical formulations, is suggested for the process of dermal repair.

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“…18,19,41 At 14 days, the qualitative histological analysis showed a predominance of thick fibers (type I) in the group LED + D, when compared with the other groups, which is characteristic of the process of fibrillogenesis. 4,40,42 Moreover, in this experimental period, the animals in the groups treated with phototherapy (LED and LED + D) had statistically significant morphometric results regarding the deposition of collagenous fibers, when compared with the control. Considering the data obtained at this experimental time for the LED group, this result is probably due to the action of phototherapy, making energy available to the cells that were damaged by the surgical process, thus accelerating the repair process during its secondary phase.…”

Section: Discussionmentioning

confidence: 71%

“…3,10 The effect of the treatment used to accelerate tissue repair can be assessed by quantifying and differentiating the collagenous fibers in the dermis at various stages of the healing process. [5][6][7]40 Thus, the results of this study show the applicability of FT-IR for the analysis and monitoring of the process of tissue repair in the experimental model using rats. ).…”

Section: Discussionmentioning

confidence: 75%

Comparative Study of Morphometric and Fourier Transform Infrared Spectroscopy Analyses of the Collagen Fibers in the Repair Process of Cutaneous Lesions

Cronemberger

Raniero

Bueno

et al. 2016

Advances in Wound Care

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Objective: Compare the efficacy of light-emitting diode (LED) and therapeutic ultrasound (TUS), combined with a semipermeable dressing (D), at forming collagen in skin lesions by morphometry and Fourier transform infrared spectroscopy (FT-IR). Materials and Methods: Surgical skin wounds (2.5 cm) were created on 84 male Wistar rats divided into four groups (n = 21): Group I (Control), Group II (LED), Group III (LED + D), and Group IV (US + D). On days 7, 14, and 21, the tissue samples were removed and divided into two pieces, one was used for histological examination (collagen) and the other for FT-IR. Results: The histomorphometric analysis showed no significant differences among groups for collagen deposition at 7 days. However, at 14 days, more deposition of collagen was noted in the groups LED ( p < 0.05) and LED + D ( p < 0.001) than in the control. At 21 days, the groups LED, LED + D, and US + D presented significantly greater deposition of collagen when compared with the control. The FT-IR spectra, at 14 days, LED + D had greater amounts of type I collagen, a better organization of fibers, and greater difference of mean separation between the groups, not observed at 7 and 21 days. Innovation: The histomorphometric and FT-IR analyses suggest that the association of semipermeable dressing to LED therapy and to TUS modulates biological events, increasing fibroblast/collagen response and accelerating dermal maturation. Conclusion: The histomorphometric and FT-IR analyses showed that LED therapy is more efficacious than TUS, when combined with a semipermeable dressing, and induced the collagen production in skin lesions.

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Análise morfométrica do colágeno dérmico a partir da segmentação por conglomerados (clusters) de cor

Cited by 26 publications

References 9 publications

Low-level laser therapy (904nm) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin

Low-level laser therapy (904nm) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin

Effect of a Semisolid Formulation ofLinum usitatissimumL. (Linseed) Oil on the Repair of Skin Wounds

Comparative Study of Morphometric and Fourier Transform Infrared Spectroscopy Analyses of the Collagen Fibers in the Repair Process of Cutaneous Lesions

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