Effect of a Semisolid Formulation ofLinum usitatissimumL. (Linseed) Oil on the Repair of Skin Wounds

Abstract

The purpose of this study was to investigate the effects of a semisolid formulation of linseed oil, SSFLO (1%, 5%, or 10%) or in natura linseed oil on skin wounds of rats. We used wound models, incisional and excisional, to evaluate, respectively, the contraction/reepithelialization of the wound and resistance to mechanical traction. The groups (n = 6) treated with SSFLO (1% or 5%) began the process of reepithelialization, to a significant extent (P < .05), on the sixth day, when compared to the petroleum jelly control group. On 14th day for the groups treated with SSFLO (1% or 5%), 100% reepithelialization was found, while in the petroleum jelly control group, this was only 33.33%. Our study showed that topical administration of SSFLO (1% or 5%) in excisional wounds allowed reepithelialization in 100% of treated animals. Therefore, a therapeutic potential of linseed oil, when used at low concentrations in the solid pharmaceutical formulations, is suggested for the process of dermal repair.

Effect of Topical Linum usitatissimum on Full Thickness Excisional Skin Wounds

“…The healing of skin wounds is a complicated process variety of cell types play roles during the proliferation process as well as migration, synthesis of the matrix, and wound contraction (10). Reduced cell and growth factor response, causing less peripheral blood flow and local angiogenesis, can lead to a delayed wound healing process in diabetics (23).…”

Effect of Semisolid Formulation of Persea Americana Mill (Avocado) Oil on Wound Healing in Rats

“…Fatty acids (oleic, linoleic, and linolenic) have been the subject of several studies, because they seem to be active in the healing process [10, 38]. The healing process can be monitored by assessing the rate of contraction of the wound, period of reepithelialization, tensile strength, and histopathology in different wound models [46].…”

“…The cutaneous wound model was performed as described previously [37, 38]. Twenty-four Wistar rats, male and female, were intramuscularly anesthetized with xylazine (3 mg kg −1 ) and ketamine (10 mg kg −1 i.m.)…”

“…A circular area of approximately 78.5 mm 2 of skin (subcutaneous tissue and fascia) was surgically removed using straight iris scissors and Adson forceps. A containment ring made of nontoxic and hypoallergenic silicone was sutured into place, with six stitches arranged symmetrically using 4.0-nylon monofilament, so that the surgical wound would remain in the center [38]. …”

“…Area of wounds at each time point was determined by the formula: A = π · R · r , where “ A ” represents the area (mm 2 ), “ R ” larger radius, and “ r ” smaller radius [38]. Thus, percentage wound contraction at each time point was derived by the following formula: percent wound contraction = (initial wound area − current area)/initial wound area × 100% [40].…”