Citocompatibilidade de blendas de poli(p-dioxanona)/ poli(hidroxi butirato) (PPD/PHB) para aplicações em engenharia de tecido cartilaginoso

Abstract

Resumo: Buscando estratégias que repercutam na melhoria da interação entre materiais poliméricos biorreabsorvíveis e o crescimento celular, o presente estudo in vitro teve como objetivo estudar a influência de blendas de PPD/PHB na adesão celular e crescimento de fibrocondrócitos obtidos a partir de cultura primária. As blendas de PPD/PHB foram preparadas pelo método de evaporação de solvente nas composições 100/0, 60/40 e 50/50 e caracterizadas por microscopia eletrônica de varredura (MEV). Observações ultra-estruturais mostraram alterações na morfologia celular, sugerindo que os fibrocondrócitos podem responder a alterações no substrato alterando seu perfil fenotípico. As análises com MTT demonstraram que as blendas não apresentaram citotoxicidade e permitiram a adesão e proliferação dos fibrocondrócitos sobre os substratos em todas as suas composições. O ensaio colorimétrico com Sirius Red evidenciou a capacidade de manutenção da síntese de matriz extracelular colágena sobre as amostras, concluindo-se que as blendas de PPD/PHB podem ser indicadas para o cultivo celular. Palavras-chave: Blenda, poli(p-dioxanona), poli(hidroxi butirato), fibrocondrócitos. Cytocompatibility of Poly(p-dioxanone)/ Poly(hydroxybutic) (PPD/PHB) Blends to Cartilage Tissue EngineeringAbstract: In order of seek strategies to improve the interaction between bioreabsorbable polymer materials and cellular growth, this work aimed at evaluating in vitro the influence of PPD/PHB blends on cell adhesion and fibrochondrocytes growth. Fibrochondrocytes cells were obtained by primary extraction from enzymatic digestion methods. The PPD/PHB blends were prepared by casting with 100/0, 60/40 and 50/50 compositions, and were characterized by scanning electron microscopy (SEM). After 6, 48, 120 and 168 hours in culture, ultrastructural observations showed changes in cell morphology, suggesting that the fibrochondrocytes can respond to substrate modifications, changing their phenotypic profile. The MTT analyses showed that the blends did not present cytotoxicity and allowed fibrochondrocytes adhesion and proliferation on the membranes in all compositions. The colorimetric Sirius Red test revealed the capability of extracellular matrix synthesis on the blends, from which one can conclude that the PPD/PHB blends are not cytotoxic and can be indicated for cell culture.

Characterization and assessment of the genotoxicity and biocompatibility of poly (hydroxybutyrate) and norbixin membranes

“…Non-synthetic biopolymers such as polyhydroxyalkanoates, which are polymers synthesized by bacteria in the presence of renewable carbon sources, such as sugarcane, are matrix options for biomaterials, among which the polyhydroxybutyrate, C 4 H 6 O 2, can be used as a biodegradable polymer, with high potential for biocompatibility and bioreabsorption and, when implanted in living systems, do not require subsequent removal of the body and do not cause undesirable effects in the long term [10][11][12][13] .…”

Use of biodegradable polymer for development of environmental tracers: a bibliometric review

“…In this way, spreading the blend of the membrane surface improved the PHB and fiber hydrophilicity, being able to reduce the serum album adsorption to 92%, lysozyme to 73% and fibrinogen to 50%. This new composite material is promising for tissue engineering (TE) and other applications in the field, for monitoring environmental sites [66,67] .…”