AFLP and SRAP markers linked to the mj gene for root-knot nematode resistance in cucumber

Abstract

Root-knot nematodes (Meloidogyne spp.) are an important worldwide pest of cucumber (Cucumis sativus L.). Molecular markers linked to the Javanese root-knot nematode (M. javanica) resistance gene mj in cucumber may aid marker assisted selection. One-hundred AFLP (EcoRI-MseI) and 112 SRAP were used to screen resistant and susceptible parents for polymorphisms to develop molecular markers linked to the mj gene. Of the 100 AFLP primers, 92 produced bands and two yielded candidate markers (E-ATT/M-CAA and E-AAC/M-CTG). These two bands were cut off from polyacrylamide gel, cloned and sequenced. Primers designed from the sequences did not yield polymorphic bands between the parents. In addition, the sequences did not contain any restriction site or indel to be used to convert them to CAPS or SCAR markers. The two sequences obtained from polymorphic AFLP markers were used primarily to design D1F, D1R, D17F and D17R primers. SRAP forward and reverse primers were used in combination with these four specific primers to search for polymorphisms between parents. Of the 112 primer combinations 11 yielded polymorphisms between parents. MapMaker Exp 3.0 software was used to analyze the 11 markers. Two markers were identified that flanked the mj gene at distance of 16.3 and 19.3 cM. The results indicated that these markers should be useful to develop molecular markers flanking the mj gene.

Herança de resistência ao Meloidogyne incognita raça 2 em pepino

“…Walters et al (1997) relataram que resistência à M. javanica é condicionada por um gene recessivo (m j ), sendo este o único trabalho encontrado sobre modo de herança da resistência de pepino à nematoide. Devran et al (2011) encontraram marcadores moleculares fortemente ligados a este gene mj que pode facilitar a seleção de plantas resistentes sem a necessidade de inoculação. Porém, este gene não condiciona resistência a M. incognita.…”

Development of a SCAR Marker for Rapid Identification of New Kentucky Bluegrass Breeding Lines

“…preferentially amplify open reading frames (ORFs) and thus the information given by SRAP markers is more in accordance with the morphological diversity of the germplasms (Ferriol et al, 2003). SRAP markers have been widely used in several crop plants for genetic diversity analysis (Comlekcioglu et al, 2010;Ferriol et al, 2003;Zhao et al, 2009), genetic linkage map construction (Yuan et al, 2008;Xue et al, 2010;Xie et al, 2011), genes tagging (Devran et al, 2011;Zhang et al, 2011) etc. There are also some reports on turfgrass research.…”

Candidate genes underlying the quantitative trait loci for root-knot nematode resistance in a Cucumis hystrix introgression line of cucumber based on population sequencing

“…Much progress has been achieved in screening and breeding for resistance to root-knot nematodes, such as M. javanica (Treub) Chitwood, in cucumber and other Cucumis species, however, no progress in RKN resistance has been made (Devran et al 2011; Fassuliotis 1970; Walters et al 1993; Wehner et al 1990; Winstead and Sasser 1956). Though several wild species with high resistance to RKN were identified, due to serious reproductive barriers, the attempts to transfer elite resistance to the cultivars through viable interspecific hybrids between cucumber and several related resistant wild Cucumis spp.…”