Variações morfológicas de embriões somáticos obtidos a partir de inflorescências de bananeira

Filippi, Silvia; Appezzato‐da‐Glória, Beatriz; Martinelli, Adriana Pinheiro

doi:10.1590/s0103-90162001000400010

Cited by 6 publications

(5 citation statements)

References 15 publications

(13 reference statements)

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“…In addition, through microscopic analyses, they can differentiate the structures formed, the regeneration process, and characterize the globular, heart-shaped, torpedo, and cotyledonary embryogenic stages. In the in vitro regeneration process, the presence or the absence of synchrony of the somatic embryos can be observed and identification of flaws in formation of the somatic embryo, so as to prevent the production of abnormal plants 11,13 .…”

Section: Introductionmentioning

confidence: 99%

Morpho and Cytological Differentiation of Calli of Eucalyptus grandis x Eucalyptus urophylla During Somatic Embryogenesis

Batista

Mendonça

Pádua

et al. 2018

Braz. arch. biol. technol.

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The aim of this study was to induce and analyze embryogenic calli from two types of explants (leaves and meristems) of the hybrid Eucalyptus grandis x Eucalyptus urophylla. Leaves and meristems of plants kept in a nursery were disinfected and inoculated in Petri dishes containing MS culture medium supplemented with different concentrations of the growth regulator dicamba (1.13, 4.52, and 9.04 µM) and without it. At 60 days of culturing, the calli were analyzed by scanning electron microscopy and at 90 days were evaluated by light microscopy in regard to the embryogenic characteristics of the cells. Different type of calli were induced in leaf explants, designated as Type I with light yellow coloring, Type II with dark yellow coloring, and Type III of brown coloring; however, only Type I had embryogenic characteristics. In the meristematic explants, only one type of callus was induced, and it had embryogenic characteristics. At 90 days of culturing, the formation of somatic embryos in the different embryogenic stages was observed and the formation of procambium, protoderm, and ground meristem tissues. At 150 days of culturing, the concentration of 1.13 µM of dicamba was prominent in the formation of somatic embryos in the different embryogenic stages.

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Section: Introductionmentioning

confidence: 99%

Morpho and Cytological Differentiation of Calli of Eucalyptus grandis x Eucalyptus urophylla During Somatic Embryogenesis

Batista

Mendonça

Pádua

et al. 2018

Braz. arch. biol. technol.

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“…It is possible that the endogenous level of auxin in the initial explant, together with the addition of NAA in the culture media, contributed to the formation of a monopolar structure, with only a root meristem. Some authors have reported the formation of somatic embryos without shoot apical meristem (Filippi;Rodriguez, 2001).…”

Section: Callus Histological Analysismentioning

confidence: 99%

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

Rodrigues

Almeida²,

Lêdo

et al. 2020

Ciênc. agrotec.

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Vernonia condensata Baker has been traditionally used in folk medicine for the treatment of several inflammatory and infectious processes. Overexploitation of this plant species has drastically reduced its population in its natural habitat (Cerrado). Therefore, tissue culture tools, such as somatic embryogenesis, can be used as an alternative method for rapid and large-scale plant regeneration. The objectives of this study were to induce callogenesis in Vernonia condensata from different types of explants and to evaluate the structural aspects of the development of pro-embryogenic masses of this species by means of histological analyses. The formation of calli was induced from leaf explants and internodal segments, which were inoculated in EME medium supplemented with 50 g L-1 sucrose, 0.5 g L-1 malt extract and 2.68 μM NAA, plus varying concentrations of BAP (0.00, 2.22, 4.44 or 8.88 μM). After 40 days, the following morphogenetic traits were evaluated: intensity of callus formation, intensity of oxidation, callus texture, and morphogenesis. The calli with embryogenic masses were analyzed by light and scanning electron microscopy. Both types of explants were responsive regarding callogenesis, with the BAP concentration of 4.44 μM promoting the formation of friable calli associated with a larger percentage of calli with embryogenic masses. Cells from leaf explants and internodal segments were able to dedifferentiate and change into embryonic structures.

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“…is restricted by the short availability of embryogenically competent explants, due to partenocarpy. In view of this, somatic embryogenesis is usually based on immature male (Filippi et al 2001;Houllou et al 2005;Pérez and Rosell, 2008) or female (Grapin et al 2000) flowers, shoot apexes or multiple meristems (Xu et al 2005;Strosse et al 2006) and long periods are required to prepare the tissues for the induction of the process.…”

Section: Scalp Inductionmentioning

confidence: 99%

Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plants

Ramirez-Villalobos¹,

García²

2008

Electron. J. Biotechnol.

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Abbreviations: 2.4-D: 2.4-dichlorophenoxiacetic acid BA: benzyladenine CE: conversion of embryos CNEC: compact non-embryogenic calluses ECS: embryogenic cell suspension ES: explants with scalps IAA: indoleacetic acid ME: mature embryos MM: maturation medium MS: Murashige and Skoog medium NECS: non-embryogenic cell suspension NNEC: nodular non-embryogenic calluses NS: number of scalps cm-2 of the multiple meristem mass. PSE: primary somatic embryos SCV: sedimented cell volumen VP: percentages of vitroplants thus obtained. The purposes of this work were to obtain embryogenic cell suspensions (ECS) from scalps and to regenerate plants of the banana CIEN-BTA-03. Shoot apexes were grown in the scalp-induction medium of Murashige and Skoog plus BA and IAA, following four diverse treatments. The first two, ME22 and ME25, were solid media supplemented with (mg L-1) 22.7 BA plus 0.192 IAA, and 25 BA plus 0.217 IAA, respectively, all containing 1.8 g L-1 of phytagel, and subcultures were performed monthly and bimonthly over 16 months. The other two treatments, IT22 and IT25, resembled ME22 and ME25 but consisted in temporary immersion for four months without subcultures, followed by two *Corresponding author months in solid media. The scalps were grown in callusinduction medium and embryogenic calluses were obtained with abundant somatic embryos, especially in scalps from IT25. About 10 to 15 embryos from each were transferred to 5 ml of multiplication medium to initiate the ECS. The scalps obtained from the IT25 treatment were the most successful as they led to ECS with high embryogenic capability. In addition, IT25 decreased the timespan required for the production of scalps. The obtained ECS gave rise to secondary somatic embryos. It showed a high multiplication index, as well as numerous mature somatic embryos, and good conversion of embryos and plant regeneration.

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Variações morfológicas de embriões somáticos obtidos a partir de inflorescências de bananeira

Cited by 6 publications

References 15 publications

Morpho and Cytological Differentiation of Calli of Eucalyptus grandis x Eucalyptus urophylla During Somatic Embryogenesis

Morpho and Cytological Differentiation of Calli of Eucalyptus grandis x Eucalyptus urophylla During Somatic Embryogenesis

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plants

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