Concentração de bap e a eficiência de micropropagação de bananeira tetraplóide (grupo AAAB)

Oliveira, R. P. de; Silveira, Daniela Garcia; Silva, Sebastião de Oliveira e

doi:10.1590/s0103-90162001000100013

Cited by 23 publications

(18 citation statements)

References 8 publications

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“…For the height characteristic (H) shown in Figure 2B, there was a quadratic effect where height increased according to the increase in BAP concentration to the concentration of 8.71 μmol L -1 , with a maximum height of 32.70 mm; from this concentration, there was a fall in plant height. Oliveira et al (2001), studying banana tree cultivar FHIA-01 (Musa sp., AAAB Group), in which the height of the seedlings during multiplication was inversely proportional to the BAP concentration, observed that the seedlings obtained in the BAP concentrations of 5.0 mg.L -1 and especially of 7.5 mg.L -1 presented a reduced size, always inferior to 1.5 cm. The inhibitory effect of BAP on shoot height was also reported in the in vitro of the "macela" (DINIZ et al, 2003), of vine (LUCAS et al, 2006), of Prunaceas (LEONTIEV-ORLOV et al, 2000) and peach trees (ROGALSKI, 2002).…”

Section: • Analysis At 30 Daysmentioning

confidence: 99%

“…According to Oliveira et al (2001), these small seedlings are not desirable in the micropropagation process because they need to undergo an elongation before rooting, so that there are no high losses in acclimatization As at 30 days, the 60-day analysis showed that the increase in BAP concentrations positively influenced the characteristics evaluated up to a certain concentration, from which there was an opposite effect, which can be explained by the endogenous level of cytokinin in the microshoots, causing excessive high concentrations to the plants in vitro.…”

Section: • Analysis At 30 Daysmentioning

confidence: 99%

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Growth of ‘Prata-Ana’ Banana’s Microshoots Clone Gorutuba From Synthetic Seeds:substrates and Bap Concentration

et al. 2017

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-The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of 'Prata-anã' banana's microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl 2 .2H 2 O (100 mM) for complexation and then in KNO 3 solution (100 mM) to decomplex. The experimental design was completely randomized in a 2 x 5 factorial design (substrate x BAP concentrations), containing different substrates (MS culture medium and vermiculite) and BAP concentrations (2.22, 4.44, 6.66, 8.88 and 13.32 μmol L -1 ) associated with NAA (naphthalene acetic acid) 0.54 μmol L -1 , totaling 10 treatments, with 4 replicates, and that each replicate containing 5 seeds. The evaluations of conversion, number of leaves, leaf length, leaf height, number of roots, root length and oxidation were performed at 30 and 60 days.The use of the MS medium provided better growth results in relation to vermiculite as substrate, in which the different BAP concentrations did not differ from each other. It was found that, in MS culture medium, BAP concentrations above 8.88 μmol L -1 in the capsule composition are not indicated for microshoots growth. Index terms: encapsulation, micropropagation, Musa sp., growth regulators. CRESCIMENTO DE MICROBROTOS DE BANANEIRA 'PRATA-ANÃ' CLONE GORUTUBA A PARTIR DE SEMENTES SINTÉTICAS: SUBSTRATOS E CONCENTRAÇÃO DE BAPRESUMO-A bananicultura destaca-se como atividade de grande importância social e econômica no Brasil, o qual ocupa o quinto lugar na produção mundial. A produção de semente sintética vem tornando-se promissora para a micropropagação e conservação in vitro. O objetivo deste trabalho foi analisar a conversão e o crescimento de microbrotos de bananeira 'Prata-anã' clone Gorutuba a partir de semente sintética em meio de cultura MS e vermiculita, testando-se diferentes substratos e concentrações de BAP (6-benzilaminopurina), associado a ANA (ácido naftaleno acético), na constituição de sua cápsula. Os microbrotos foram mergulhados à matriz de alginato de sódio (3%) e gotejados em solução de CaCl2. 2H2O (100 mM) para complexação e depois em solução de KNO3 (100 mM) a descomplexação. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 2 x 5 (substrato x concentrações de BAP), contendo diferentes substratos (meio de cultura MS e vermiculita) e concentrações de BAP (2,22; 4,44; 6,66; 8,88 e 13,32 μmol L -1 ), associado a ANA (ácido naftalenoacético) 0,54 μmol L -1 , totalizando 10 tratamentos, com 4 repetições, sendo que cada repetição conteve 5 sementes. As avaliações de conversão, nú...

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Section: • Analysis At 30 Daysmentioning

confidence: 99%

Section: • Analysis At 30 Daysmentioning

confidence: 99%

Growth of ‘Prata-Ana’ Banana’s Microshoots Clone Gorutuba From Synthetic Seeds:substrates and Bap Concentration

et al. 2017

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“…However, at the same time, the shoots were of a shorter length. This contradictory behavior is explained by the fact that the exacerbated increase in the number of shoots would have promoted competition for the salts and vitamins in the culture medium, thus reducing growth (OLIVEIRA et al, 2001).…”

Section: +mentioning

confidence: 99%

In vitro multiplication of pear tree cultivar Cascatense

et al. 2016

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One main obstacle to the development and expansion of pear tree cultivation in Brazil has been the lack of cultivars adapted to the edaphoclimatic conditions of regions with the potential for pear production. Intending to overcome this hindrance, Embrapa Clima Temperado (Embrapa Temperate Climate) developed and released the cultivar Cascatense, with a low cold requirement (~400 h) better suited for Rio Grande do Sul`s and Santa Catarina`s climate. Still, seedlings of high genetic and sanitary quality are necessary for the establishment of a high-yield orchard. Plants with both sanitary quality and uniformity can be obtained through in vitro micropropagation. Thus, the present study aimed to assess the influence of different culture media (MS, MS¾, WPM, QL, and Himédia), concentrations of sucrose (0, 15, 30, 45, and 60 g L -1 ), and concentrations of benzylaminopurine (BAP) (0, 0.4, 0.8, 1.2, and 1.6 mg L -1 ) upon the in vitro multiplication of cultivar Cascatense, to establish an efficient protocol to enhance its propagation and reproducibility. The study included three sequential experiments in which the number of shoots per explant, the length of shoots, the number of axillary buds per shoot, fresh mass, and dry mass of shoots were evaluated. Cultivar Cascatense can be best propagated in vitro with the use of Himédia medium, supplemented with 0.8 mg L ResumoUm dos principais entraves ao desenvolvimento e expansão da cultura da pereira no Brasil tem sido a falta de cultivares adaptadas às condições edafoclimáticas das regiões potencialmente produtoras. Visando sanar esta dificuldade, a Embrapa Clima Temperado desenvolveu e lançou a cv. Cascatense, com baixa exigência em frio (~400 h), mais adequada ao clima do Rio Grande do Sul e de Santa Catarina. No entanto, mudas com alta qualidade genética e sanitária são necessárias para garantir a boa formação de um pomar produtivo. Plantas com qualidade sanitária e uniformidade podem ser obtidas através da micropropagação in vitro. Assim, o presente trabalho teve como objetivo verificar a influência de diferentes meios de cultivo (MS, MS¾, WPM, QL e Himédia), concentrações de sacarose (0, 15, 30, 45 e 60 g L -1 ) e benzilaminopurina -BAP (0; 0,4; 0,8; 1,2 e 1,6 mg L -1 ) na multiplicação in vitro da cv. Cascatense, visando obter um protocolo eficiente para potencializar sua propagação e reprodutibilidade. Os estudos foram conduzidos em três experimentos sequenciais, nos quais foram avaliados o número de brotações por explante, o comprimento das brotações, o número de gemas axilares por brotação, massa fresca e massa seca das brotações. A cv. Cascatense pode ser propagada in vitro com a utilização do meio Himédia suplementado com 0,8 mg L -1 de BAP e 30 g L -1 de sacarose. Palavras-chave: Meio himédia. Sulfato ferroso. Sulfato férrico. Sacarose. Benzilaminopurina.

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“…Outros trabalhos têm utilizado diluições de sais minerais nos meios de cultura em diversas frutíferas, tais como maçã (Pasqual et al, 2000), maracujá (Biasi et al, 2000), banana (Oliveira et al, 2001) e citros , com resultados positivos no enraizamento.…”

Section: Comprimento Do Explante (Cm) Explantes Responsivos (%)unclassified

Regeneração de plantas de laranja 'Pêra' via organogênese in vitro

Silva

Costa

Souza

et al. 2005

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi estabelecer um protocolo eficiente de regeneração de plantas in vitro, via organogênese em explante juvenil de laranja 'Pêra' (Citrus sinensis L. Osbeck), para atender futuros trabalhos de transformação genética. Segmentos de epicótilo utilizados como explantes foram introduzidos em meio de cultura MT. A fim de maximizar a regeneração de plantas in vitro, foram realizados experimentos para avaliar as concentrações de BAP no meio de cultivo (0, 1, 2, 3 ou 4 mg L -1 ), tamanho (0,25, 0,5 ou 1 cm), polaridade (basal, medial e apical), posição (horizontal ou vertical), condições de luminosidade (fotoperíodo de 16 horas e escuro por 30 dias) e seccionamento nas extremidades dos explantes, bem como melhores condições para garantir plantas enraizadas (meio MT, MT/2, com ou sem auxina e microenxertia). A combinação de 3 mg L -1 de BAP com segmentos de 0,25 cm de comprimento foi eficiente na resposta organogenética. Segmentos apicais e mediais apresentaram melhores resultados do que os basais. O cultivo dos segmentos na posição horizontal, em fotoperíodo de 16 horas, foi mais eficiente do que na posição vertical. Não houve melhora na indução da organogênese in vitro, quando foram seccionadas as extremidades dos explantes. A microenxertia assegurou 100% de brotos enraizados.Termos para indexação: Citrus sinensis, cultura de tecidos, explante juvenil, benzilaminopurina, microenxertia. Regeneration of 'Pera' sweet orange plants through in vitro organogenesisAbstract -The objective of this work was to establish an efficient protocol for in vitro regeneration of plants through organogenesis of sweet orange 'Pera' (Citrus sinensis L. Osbeck) juvenile explants, in order to give support for future studies of genetic transformation. Epicotyl segments used as explants were introduced in MT culture media. To maximize the regeneration of in vitro plants, experiments were performed to evaluate different concentrations of BAP in the culture media (0, 1, 2, 3 or 4 mg L -1 ), size (0.25, 0.5 or 1 cm), polarity (basal, medium, and apical), position (horizontal or vertical), luminosity conditions (photoperiod of 16 hours, and darkness for 30 days), and incision of the segment ends. In addition, better conditions for obtaining rooted plants (culture media MT, MT/2, with and without auxin and micrografting) were studied. The combination of 3 mg L -1 BAP with segments of 0.25 cm length was efficient for the organogenetic response. Segment apices and mediums were more efficient comparing to the basal parts. Culturing the segments in the horizontal position, with a photoperiod of 16 hours, was more efficient than in the vertical position. There was no improvement in the induction of in vitro organogenesis, when the explant ends were incised. Micrografting assured 100% of rooted shoots.

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Concentração de bap e a eficiência de micropropagação de bananeira tetraplóide (grupo AAAB)

Cited by 23 publications

References 8 publications

Growth of ‘Prata-Ana’ Banana’s Microshoots Clone Gorutuba From Synthetic Seeds:substrates and Bap Concentration

Growth of ‘Prata-Ana’ Banana’s Microshoots Clone Gorutuba From Synthetic Seeds:substrates and Bap Concentration

In vitro multiplication of pear tree cultivar Cascatense

Regeneração de plantas de laranja 'Pêra' via organogênese in vitro

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