Controle da vitrificação do cravo (Dianthus caryophyllus L.) in vitro

Cuzzuol, Geraldo Rogério Faustini; Gallo, Luiz Antonio; Almeida, Marcílio de; Crocomo, Otto J.

doi:10.1590/s0103-90161995000300030

Cited by 3 publications

(2 citation statements)

References 27 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Resultado similar foi obtido por Santana et al (2008) que obtiveram melhores taxas de enraizamento in vitro de brotos de Annona glabra L. em meio de cultura sem a presença de sacarose, porém com tubos vedados com tampão de algodão e com tampa plástica sem a película de PVC. Já Cuzzuol et al (1995) Conforme se observa na Tabela 3 os resultados da análise de variância para o experimento 2 mostraram um efeito significativo da interação entre as concentrações de sacarose e dos sais do MS (p<0,01) para as variáveis, número de folhas (NF) e comprimento (cm) da parte aérea (CPA). Para a variável número de raízes (NR) a interação não foi significativa ainda que os fatores isoladamente tenham influenciado nas respostas obtidas.…”

Section: Resultsunclassified

Aspectos morfofisiológicos na pré-aclimatização in vitro e aclimatização de plantas de caroá

et al. 2013

View full text Add to dashboard Cite

RESUMO -), tipo de vedação (filme de PVC e tampão de algodão) dos tubos de ensaio, e a concentração dos sais do meio MS (50; 75 e 100%) sobre os aspectos morfofisiológicos das plantas de caroá durante a fase de pré-aclimatização in vitro visando otimizar a taxa de sobrevivência e reduzir o tempo de aclimatização das mudas. A análise das características de comprimento, produção de matéria seca, taxa de sobrevivência e enraizamento mostraram que as plantas de Neoglaziovia variegata nas fases de pré-aclimatização in vitro e aclimatização foram dependentes das concentrações de sacarose e nutrientes inorgânicos, além da aeração. Melhores resultados foram obtidos quando as mudas de caroá foram inoculadas em meio MS com a concentração original dos sais do MS, suplementado com 15 ou 30 g L -1 de sacarose e vedadas com tampão de algodão. Palavras-chave:Caroá. Tecidos vegetais-cultura e meios de cultura. Sacarose.ABSTRACT -This work aimed to evaluate the influence of sucrose (0; 15 and 30g L -1 ), the type of seal used in the test tubes (PVC film or cotton plug), and salt concentration in the MS medium (50%; 75% and 100%) on the morphological and physiological traits of caroá plants during in vitro pre-acclimatization phase aiming to optimize the survival rate and shorten the acclimatization period of the seedlings. The analysis of characteristics such as length, dry matter production, survival rate and rooting, showed that Neoglaziovia variegata plants at pre-acclimatization and acclimatization stages were dependent on sucrose concentrations, inorganic nutrients from MS medium and aeration. The best results in the acclimatization stage were obtained when the caroá seedlings were inoculated in MS medium with the full salt concentration, supplemented with 15 or 30g L -1 sucrose, and sealed with cotton plugs during the pre-acclimatization stage.

show abstract

Section: Resultsunclassified

Aspectos morfofisiológicos na pré-aclimatização in vitro e aclimatização de plantas de caroá

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Em concentrações elevadas, o BAP promove sintomas de vitrificação (MONTOVANI et al, 1999;SOUZA et al, 2003;BERTONI et al, 2006), proporciona elevada taxa de multiplicação de brotos, porém, com tamanhos reduzidos (VILLA et al, 2005), diminui consideravelmente o número de folhas VILLA et al, 2005), além de promover nestas últimas, tamanhos e formas atípicos . Sintomas de vitrificação em concentrações mais baixas do BAP também foram reportados por Cuzzuol et al (1995).…”

Section: As Auxinas Na Micropropagaçãounclassified

Avaliações morfofisiológicas do desenvolvimento de microplantas de pupunheiras submetidas a tratamentos com biorreguladores

Graner¹

View full text Add to dashboard Cite

This study aimed to evaluate the role of bioregulators (NAA, BAP, TDZ and 2iP) on the morphogenetic development of pejibaye microplants. For this, every 28 days, the number of propagules, the root development, the length and the growth rate of shoots, the number of leaves, the oxidation, the number of meristematic centers in the explants basal region and in the leaf sheaths and/or in the propagules leaf primordia, the number of pro-embryogenic cell complex and the morphogenic routes and patterns were measured. All experiments were conducted in a randomized design. Qualitative data were presented as percentages and general averages, and quantitative data were subjected to polynomial regression or exponential analysis. The morphophysiological evaluations showed that the TDZ alone favored the development of adventitious buds, although short, and sometimes with thorns. Only in the treatments with the isolated presence of BAP, TDZ and 2iP, somatic embryos were not developed. For the other treatments there was an exponential behavior, in which the number of somatic embryos was reduced and varied according only to the cultivation period. Although a greater number of roots was observed in the treatment, in which NAA was associated with BAP, the control treatment has induced the highest rate of rooted explants and with greater root length, and has been the single one that produced branches. The isolated presence of TDZ promoted the greater shoot length, and its association with ANA promoted the highest growth rate. The best shoot development of pejibaye microplants and explants occurred in the MS bioregulator-free culture medium of or in the medium with NAA/TDZ association, at140 days of cultivation. Moreover, the isolated addition of the cytokinins BAP, TDZ and 2iP in the medium, later promoted the highest growth rate. In pejibaye explants the addition of 2iP alone promoted intense oxidation of the first developed leaves and the application of TDZ alone or associated with ANA promoted anomalies such as folding leaf and thorn development. The monitoring of the morphogenetic routes and patterns showed that in the treatments that were bioregulator-free, or with the isolated presence of NAA and TDZ, or the association of both, somatic embryos, from multicellular origin, raised up directly in the central region of the explants base. Pro-embryos from unicellular origin were also induced in the isolated presence of NAA or in NAA/BAP and NAA/TDZ associations. Moreover, in the isolated presence of TDZ, pro-embryos from multicellular origin were induced from shoot apical meristem activity and from the (bi) polarization of a large number of meristematic centers in the distal extremity of the explants base. Adventitious buds with multicellular origin from the proximal extremity of the explants base were induced more frequently in the bioregulator-free treatment and in the treatment with NAA/TDZ. Between the leaf explants primordia, adventitious buds were often induced by the treatments with the isolated presence of ANA.

show abstract

Caracterização isozimática e atividade de peroxidase em folhas de plantas hiperídrica, intermediária e normal de Bidens pilosa L. mantidas in vitro

2008

View full text Add to dashboard Cite

RESUMOForam caracterizadas as plantas: hiperídrica, intermediária e normal de um clone de Bidens pilosa mantido em cultivo in vitro por meio de isozimas e da atividade de peroxidase. Empregando-se a eletroforese em géis de amido a 12%, testou-se seis isozimas, sendo detectado polimorfismo em peroxidase e fosfatase ácida, permitindo caracterizar cada tipo de planta. Não houve polimorfismo em fosfogluco isomerase, fosfoglucomutase, glutamato oxaloacetato transaminase e malato desidrogenase. A atividade da peroxidase foi maior nas plantas hiperídricas e intermediárias. Conclui-se que a variabilidade enzimática tem potencial como marcador de hiperidricidade em plantas mantidas in vitro.Termos para indexação: Isozima, hiperidricidade, vitrificação. ABSTRACTActivity of peroxidase (EC 1.11.1.7) and isozymes analysis of a Bidens pilosa clone maintained in vitro culture were characterized in hyperhydric, intermediary and normal plants. Electrophorese in starch gels (12%) of six isozymes systems was tested, polymorphisms in peroxidase and acid phosphatase (EC 3.1.3.2) were detected. There was absence of polymorphism in phosphoglucoisomerase (EC 5.3.1.9), phosphoglucomutase (EC 5.4.2.2), glutamate oxaloacetate transaminase (EC 2.6.1.1) and malate dehydrogenase (EC 1.1.1.37). Comparing the activity of peroxidase enzyme, it was higher in hyperhydric and intermediary plants in relation to normal ones. Enzymatic variability is a potential tool as hyperhydricity marker in plants grown in vitro.

show abstract

Controle da vitrificação do cravo (Dianthus caryophyllus L.) in vitro

Abstract: RESUMO -Baixos níveis de benziladenina (BAP),

Cited by 3 publications

References 27 publications

Aspectos morfofisiológicos na pré-aclimatização in vitro e aclimatização de plantas de caroá

Aspectos morfofisiológicos na pré-aclimatização in vitro e aclimatização de plantas de caroá

Avaliações morfofisiológicas do desenvolvimento de microplantas de pupunheiras submetidas a tratamentos com biorreguladores

Caracterização isozimática e atividade de peroxidase em folhas de plantas hiperídrica, intermediária e normal de Bidens pilosa L. mantidas in vitro

Contact Info

Product

Resources

About