Aspectos morfofisiológicos na pré-aclimatização in vitro e aclimatização de plantas de caroá

Abstract

RESUMO -), tipo de vedação (filme de PVC e tampão de algodão) dos tubos de ensaio, e a concentração dos sais do meio MS (50; 75 e 100%) sobre os aspectos morfofisiológicos das plantas de caroá durante a fase de pré-aclimatização in vitro visando otimizar a taxa de sobrevivência e reduzir o tempo de aclimatização das mudas. A análise das características de comprimento, produção de matéria seca, taxa de sobrevivência e enraizamento mostraram que as plantas de Neoglaziovia variegata nas fases de pré-aclimatização in vitro e aclimatização foram dependentes das concentrações de sacarose e nutrientes inorgânicos, além da aeração. Melhores resultados foram obtidos quando as mudas de caroá foram inoculadas em meio MS com a concentração original dos sais do MS, suplementado com 15 ou 30 g L -1 de sacarose e vedadas com tampão de algodão. Palavras-chave:Caroá. Tecidos vegetais-cultura e meios de cultura. Sacarose.ABSTRACT -This work aimed to evaluate the influence of sucrose (0; 15 and 30g L -1 ), the type of seal used in the test tubes (PVC film or cotton plug), and salt concentration in the MS medium (50%; 75% and 100%) on the morphological and physiological traits of caroá plants during in vitro pre-acclimatization phase aiming to optimize the survival rate and shorten the acclimatization period of the seedlings. The analysis of characteristics such as length, dry matter production, survival rate and rooting, showed that Neoglaziovia variegata plants at pre-acclimatization and acclimatization stages were dependent on sucrose concentrations, inorganic nutrients from MS medium and aeration. The best results in the acclimatization stage were obtained when the caroá seedlings were inoculated in MS medium with the full salt concentration, supplemented with 15 or 30g L -1 sucrose, and sealed with cotton plugs during the pre-acclimatization stage.

Nutrient concentrations of in vitro culture is crucial for Desmodium incanum DC. Acclimatization

“…Corroborating these results, Oliveira et al confirmed in 2010 that the composition of the culture medium is essential for the survival ornamental pineapples pre-acclimatization, especially with regard to rooting. Moreover, similar to our results, Caroá plants from MS culture medium modified, had its survival percentage favored by low concentrations of salts (Silveira et al, 2013).…”

Controlling hyperhydricity in micropropagated plants of Lippia grata Schauer (Verbenaceae), a native species of a dry seasonal tropical forest with pharmacological potential

“…The lower leaf hyperhydricity rate found in the partial sealing treatments may be related to the gas exchange provided by the partial opening in the test tubes, similar to what was found in Neoglaziovia variegata (Arruda) Mez (Silveira et al 2013). The ventilation in the flasks leads to reduced humidity in vitro and allows removing the gases accumulated in the culture vessel, in addition to providing the increase in CO 2 concentration, favoring leaf expansion, greater photosynthetic activities and preventing physiological disturbance (de Cruz et al 2013;Pinheiro et al 2013;Gao et al 2017).…”

Asymbiotic germination, initial development in vitro and acclimatization of Cyrtopodium paludicolum Hoehne, a Brazilian Savanna orchid species

“…The process of acclimatization refers to a set of procedures by which plants produced under controlled in vitro conditions are progressively transferred to a natural environment. Many individuals do not survive this process primarily because their root systems are scantily branched, are easily damaged during transfer and do not bear root hairs which make roots poorly functional in terms of water and nutrient absorption (Silveira et al 2013).…”