Expression of matrix metalloproteases-2 and -9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment

Laskoski, Luciane Maria; Valadão, Carlos Augusto Araújo; Vasconcelos, Rosemeri de Oliveira; Ferrucci, Danilo Lopes; Silva, Juliete Aparecida Francisco; Machado, Dagmar Ruth Stach; Dória, Renata Gebara Sampaio; Mendonça, Fábio de Souza

doi:10.1590/s0103-84782013000100012

Cited by 4 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the bacterial toxins that activate MMPs, exotoxins of Streptococcus species, particularly Streptococcus bovis assume great importance (POLLITT, 1999). Nonetheless, the increase of the substance may occur as a result of the morphological alteration of the laminar tissue, and not to be the cause of the disease (LASKOSKI et al, 2013).…”

Section: Enzyme Mechanismsmentioning

confidence: 99%

“…The development phase of laminitis presents various inflammatory events such as elevated acute phase protein concentrations (FAGLIARI et al, 1998), inflammatory cytokines (LOFTUS et al, 2007), reactive oxygen species (HUURLEY et al, 2006) as well as leukocyte infiltration into tissue (HURLEY et al, 2006;FALEIROS et al, 2009a, LASKOSKI et al, 2013. Systemic inflammatory activation may be from infectious origin such as exposure to pathogen-associated molecular patterns (PAMPs), or even, due to extensive tissue damage associated with endogenous release of the damage associated molecular patterns (DAMPs).…”

Section: Inflammatory Mechanismsmentioning

confidence: 99%

See 1 more Smart Citation

An update on equine laminitis

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Enzyme Mechanismsmentioning

confidence: 99%

Section: Inflammatory Mechanismsmentioning

confidence: 99%

An update on equine laminitis

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

Transcriptome diversity and differential expression in supporting limb laminitis

Holl

Armstrong

Galantino‐Homer

et al. 2022

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

Endotoxin-induced changes of type VII collagen– cleaving matrix metalloproteinases in lamellar tissue of extracorporeally perfused equine limbs

Patan-Zugaj¹,

Gauff²,

Egerbacher³

et al. 2018

ajvr

View full text Add to dashboard Cite

OBJECTIVE To investigate the effect of lipopolysaccharide (LPS) on type VII collagen- cleaving matrix metalloproteinases (MMPs) in the lamellar tissue of extracorporeally perfused equine limbs. SAMPLE 10 right forelimbs and 3 left forelimbs collected from 10 adult horses after slaughter at a licensed abattoir. PROCEDURES Extracorporeal perfusion of the isolated equine limbs was performed for 10 hours under physiologic conditions (control-perfused limbs; n = 5) and with the addition of 80 ng of LPS/L of perfusate (LPS-perfused limbs; 5). Lamellar tissue specimens were then collected from the dorsal aspect of the hooves. Additionally, corresponding control specimens were collected from the 3 nonperfused left forelimbs. Immunohistochemical analysis was performed on paraffin-embedded tissue blocks with antibodies against total (latent and active) MMP-1, MMP-2, MMP-8, and MMP-9 as well as antibody against active MMP-9. Intensity of immunohistochemical staining was scored, and stain distribution in the lamellar tissue was noted. RESULTS Staining intensity of total and active MMP-9 was significantly increased in LPS-perfused versus control-perfused limbs. No such difference was identified for MMP-1, MMP-2, and MMP-8. CONCLUSIONS AND CLINICAL RELEVANCE Of the 4 MMPs that are capable of degrading type VII collagen, MMP-9 was the only one for which production increased in the lamellar tissue of isolated equine limbs perfused with versus without a clinically relevant concentration of LPS. These results suggested that MMP-9 may be involved in initiation of pathological changes in lamellar tissue in endotoxin-induced laminitis, whereas MMP-1, MMP-2, and MMP-8 may be less relevant.

show abstract

Expression of matrix metalloproteases-2 and -9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment

Abstract: Twenty horses were used in the experiment

Cited by 4 publications

References 23 publications

An update on equine laminitis

An update on equine laminitis

Transcriptome diversity and differential expression in supporting limb laminitis

Endotoxin-induced changes of type VII collagen– cleaving matrix metalloproteinases in lamellar tissue of extracorporeally perfused equine limbs

Contact Info

Product

Resources

About