Desinfestação superficial, germinação e regeneração in vitro a partir de sementes de calêndula

In Vitro Germination of Melanoxylon Brauna Schott. And Evaluation of the Toxicity of Disinfecting Agents in the Lactuca Sativa L. Model Plant

“…It is believed that the absence of the nuclear envelope makes proteins and nucleic acids more vulnerable to NaOCl, since this disinfecting agent acts on these molecules. Bevilacqua et al (2011) ratified the efficiency of the antibacterial action of NaOCl in seeds of Calendula officinalis, which after being treated with 2.5% NaOCl for 30 minutes had 93.9% of the seeds free of this microorganism.…”

<b>Establishment and content of sugars and phenols in <i>Physalis</i> callus obtained from different explants and concentrations of bap and naa </b> - doi: 10.4025/actascibiolsci.v36i1.18074

“…According to Al Khateeb et al (2012) high concentrations of indole butyric acid (IBA) or NAA inhibit the formation of roots in callus obtained from leaf explants of C. pumilum Jacq. In the case of seeds of marigold, the rooting occurred in the absence of BAP, without the need to add NAA, and under its presence there was a decrease in the process (BEVILACQUA et al, 2011). In D. multiflora, authors did not register satisfactory results in inducing the rooting, using NAA or IBA in several concentrations (SOUZA et al, 2011).…”

DISINFECTION PROTOCOL AND IN VITRO GERMINATION OF SEEDS OF Dalbergia nigra

“…This is an important disinfecting agent since the chlorine present in the molecule combines with proteins in the cell membranes of the microorganism, forming toxic compounds that inhibit essential enzymes for its proliferation (Donini et al, 2005). For example, the antibacterial efficiency (93%) of the use of NaOCl (2.5%), for 30 min, was proven in seeds of Calendula officinalis L. (Bevilacqua et al, 2011).…”