Comparação de métodos diretos de extração e quantificação dos teores de clorofilas em folhas do capim-Tifton 85

Barbieri, Élio; Rossiello, Roberto Oscar Pereyra; Morenz, Mirton José Frota; Ribeiro, Roberta Cristiane

doi:10.1590/s0103-84782010000300022

Cited by 15 publications

(9 citation statements)

References 11 publications

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“…The absorbances were measured at 470, 647, and 663 nm, and chlorophyll a and b concentrations were calculated through the equations proposed by Barbieri Junior et al (2010). Free proline was quantified using 0.1g of fresh leaf tissue assessed by the method developed by Bates et al (1973).…”

Section: Assessmentsmentioning

confidence: 99%

Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids

et al. 2016

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ABSTRACT:Micropropagation is an alternative to produce orchid plants in large scale. However, this process presents losses during acclimatization. Exogenous proline use in vitro plant tissue culture can reduce the stress of the plant acclimatization phase. We aimed to verify the growth of orchids in different micropropagation systems with the addition of proline in the culture medium. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. Seeds were germinated in MS medium, added 20 g. L -1 of sucrose, solidified with 6 g. L -1 of agar and pH adjusted for 5,8. The cultures were incubated in a growth room with temperature of 24 ± 2 0 C, under photoperiod of 16 h. After 5 months, 1-cm long seedlings were placed in a culture vessel according to the treatments, which were composed of two micropropagation systems (conventional and natural ventilation) and three proline concentrations (0, 1, and 2 g·L -1 ). The experiment was carried out in an entirely randomized design consisting of a 2 × 3 factorial, for a total of 6 treatments, each with 5 replicates. The natural ventilation system with the use of proline (1 g·L -1 ) promoted higher dry mass accumulation and better control of water loss by plants.

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Section: Assessmentsmentioning

confidence: 99%

Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids

et al. 2016

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“…Acetone is not suitable for field work due to volatility, propensity to leak from containers, flammability and despite the best results observed, it is permissible to lose a certain precision and use another method under field conditions, when it involves the comparison of a high number of treatments and repetitions (Ritchie, 2008;Barbieri Junior et al, 2010). Protocols, where part of the plant tissue is immersed in a solvent, are recommended due to the practicality, the possibility of preparing a large number of samples in a short time.…”

Section: Resultsmentioning

confidence: 99%

Spectrophotometric Determinations of Chloroplastidic Pigments in Physalis angulata L. Leaves Using Different Methodologies

Tanan¹,

Nascimento²,

Leite³

et al. 2017

JAS

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The aim of this study was to compare different protocols for extraction and quantification of photosynthetic pigments, considering different solvents, extraction time and temperature, to determine the best methodology for Physalis angulata L. leaves. The efficiency of immersion of leaf discs in 95% ethanol, 80% acetone and dimethyl sulfoxide (DMSO) was evaluated for 24, 48 and 72 hours. The effect of temperature on extraction with DMSO was also evaluated, with the samples incubated at room temperature and in a water bath at 65 °C. These protocols were compared with the classical methods of plant tissue maceration in 80% acetone followed by filtration, which proved to be the most efficient for Physalis angulata plants and indicated for use in the laboratory. The use of high temperatures (65 °C) is not necessary for the chlorophyll extraction process with DMSO, the room temperature is most efficient in the extraction of carotenoids. Considering the total chlorophyll content, the leaf discs immersion technique in a solvent is acceptable, with 95% ethanol and 24 hours incubation and the most economically viable alternative.

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“…Chlorophylls and carotenoids contents can be obtained by extraction with organic solvents (Silva et al 2014) and there are several methodologies already elucidated for the extraction of these pigments in diverse vegetal species, as the use of ethanol (Wintermans andDe Mots 1965, Lichtenthaler 1987), N,N-dimethylformamide (DMF) (Moran 1982, Wellburn 1994, 80% acetone and dimethylsulfoxide (DMSO) ( Barnes et al 1992, Wellburn 1994. However, there are considerable variations in the extraction of pigments, depending on the methodology used being that the main sources of variation include: (i) nature, polarity and purity degree of the solvent; (ii) temperature and incubation/extraction time; and (iii) the equations used, which must be specific to the different solvents (Porra et al 1989, Tait and Hik 2003, Ritchie 2008, Barbieri Junior et al 2010. In addition, the species is an important source of variation regarding the extraction methodology of photosynthetic pigments, since the contents of these compounds diversifies according to the organisms.…”

Section: Introductionmentioning

confidence: 99%

Comparison between extraction methods of photosynthetic pigments in Acacia mangium

Siebeneichler¹,

Barbosa²,

Cruz³

et al. 2019

Commun. Plant Sci.

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The aim of this work was to determine a simple and rapid protocol for the extraction of photosynthetic pigments (chlorophylls and carotenoids) from Acacia mangium (Willd.), a pioneer species with rapid growth and that has been used in commercial forests in northern Brazil. The experiment consisted of 4x5 factorial, 4 solvents (ethanol, dimethylsulfoxide (DMSO), N,N-dimethylformamide (DMF), and 80% acetone) and 5 extraction times (24, 48, 72, 96, and 120 hours). The equations used for calculations varied according to the solvent used. For a quantification of the photosynthetic pigment contents of A. mangium it is recommended to use 80% acetone with 84 hours of extraction since this solvent allows the efficient extraction of both chlorophylls and carotenoids with a single protocol. Highlighted Conclusion Determination of the Acacia leaves pigments (chlorophylls and carotenoids) it is possible with a single protocol in 84 hours, using an extraction with 80% acetone.

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Comparação de métodos diretos de extração e quantificação dos teores de clorofilas em folhas do capim-Tifton 85

Cited by 15 publications

References 11 publications

Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids

Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids

Spectrophotometric Determinations of Chloroplastidic Pigments in Physalis angulata L. Leaves Using Different Methodologies

Comparison between extraction methods of photosynthetic pigments in Acacia mangium

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