Mastite micótica em ruminantes causada por leveduras

Spanamberg, Andréia; Sanches, Edna Maria Cavallini; Santúrio, Jânio Morais; Ferreiro, Laerte

doi:10.1590/s0103-84782008005000045

Cited by 20 publications

(17 citation statements)

References 34 publications

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“…In addition, these yeasts often showed a population with multiple resistance to antifungal drugs (Samaranayake & Samaranayake, 1994). The genus Candida and other yeasts may be associated with cases of clinical or subclinical mastitis in ruminants, either as primary or secondary agents (Spanamberg et al, 2009). There are also reports that Candida glabrata can be intrinsically associated with diarrhea in newborn calves (Elad et al, 1998;Radostitis et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Aerobic fungi in the rumen fluid from dairy cattle fed different sources of forage

et al. 2012

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-The objective of this study was to evaluate the aerobic microbiota of the rumen fluid from Holstein cows and heifers fed different tropical forage in the north of Minas Gerais, Brazil. A total of 30 samples of rumen fluid from cows fed with sorghum silage were collected: 32 from cows fed Brachiaria brizantha, 12 from heifers that received sorghum silage and 11 from calves fed sugar cane foliage. The culture was carried out using the agar Sabouraud medium and the solid C medium, containing microcrystalline cellulose. The isolated mycelial fungi were identified by microculture technique and yeasts by micromorphological and physical-chemical analysis. Specific identification for yeasts was confirmed by ribosomal DNA sequence analysis. The presence of fungal colonies was confirmed on the Sabouraud medium for 100% of the samples. No significant differences were observed comparing the concentrations of mycelia fungi in the rumen fluid from cows fed different forages and for the two categories evaluated, fed sorghum silage. Yeast populations in the rumen fluid from heifers fed sugarcane were higher compared with those receiving sorghum silage. The yeast Pichia kudriavzevii (Candida krusei) was the most frequent and among the mycelial fungi, the genus Aspergillus was the most frequently observed, corresponding to 56% of the samples. Future studies should elucidate the variations in the populations of these microorganisms considering the carbohydrate sources in the tropical forages and the animal categories. The ecological or pathogenic role of these microorganisms should also be considered, aiming at improved productivity and health of cattle.

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Section: Discussionmentioning

confidence: 99%

Aerobic fungi in the rumen fluid from dairy cattle fed different sources of forage

et al. 2012

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“…A mastite bovina causada por fungos leveduriformes e filamentosos é considerada uma enfermidade emergente, sendo o gênero Candida um dos principais envolvidos nesse problema (Jones;Hunt & King, 2000;Spanemberg et al, 2009a;Cruz, 2010). A partir do advento da antibioticoterapia antibacteriana nas décadas de 50 e 60, observou-se um aumento na incidência de mastites causadas por leveduras, especialmente sob a forma de surtos localizados, os quais são frequentemente relacionados ao tratamento antimicrobiano por período prolongado (Spanemberg et al, 2009b;Langoni et al, 2013).…”

Section: Introductionunclassified

Disinfectant activity of the crude hydroalcoholic extract of Achyrocline satureioides (Asteraceae) on Candida spp. isolated in problem-situations of bovine mastitis

Avancini

Campos

Valente

et al. 2016

AVB

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RESUMO -O fenômeno de resistência apresentada por leveduras aos antimicóticos bem como a necessidade de insumos de higiene para uso em sistemas de criação animal baseados no modelo agroecológico motivaram o desenvolvimento deste trabalho. Os objetivos foram realizar a análise fitoquímica qualitativa e avaliar a ação antimicrobiana/desinfetante do extrato hidroalcoólico bruto [peso:volume de 5g:100 mL (50 mg mL)] das flores de Achyrocline satureioides sobre uma Candida albicans padrão (ATCC 14053) e isoladas de casos de mastite bovina: C. albicans (n = 1) , C. krusei (n = 21) e C. rugosa (n = 9). O teste foi o de suspensão, com as linhagens em três densidades populacionais (DP): 10 5 , 10 4 e 10 3 UFC mL, nos tempos de contato (TC) de 15 e 30 minutos e de 1 até 10 horas. Com resultados da atividade influenciados pelas variáveis DP e TC, todas as leveduras foram inativadas. Como exemplo na mais alta DP: a padrão estava inativada na leitura de 1 h; dos isolados clínicos, o de C. albicans inativado na leitura das 3 h; sete isolados de C. krusei estavam inativados aos 30 min e os outros 14 na leitura de 1 h; sete isolados de C. rugosa estavam inativados na leitura das 4 h, os outros até às 10 h. Os fitoquímicos identificados foram compostos fenólicos, taninos, taninos condensados e flavonóides. As evidências observadas sugerem o potencial do extrato para uso sobre as fontes de infecção em procedimentos de desinfecção ou de antissepsia, ou em formulações que lhe sirvam de veículo.Palavras-Chave: extrato vegetal; Achyrocline satureioides; Candida spp.; produto natural.ABSTRACT -This work was prompted by the phenomenon of yeasts' resistance to antifungals, and the need for sanitation products for use in livestock farming in the agroecological model. The objectives were to undertake a qualitative phytochemical analysis of, and assess the antimicrobial/disinfectant action of, the crude hydroalcoholic extract [weight:volume of 5g:100 mL (50 mg mL)] of flowers of Achyrocline satureioides on standard Candida albicans (ATCC 14053) and isolates of cases of bovine mastitis: C. albicans (n = 1) , C. krusei (n = 21) and C. rugosa (n = 9). The test was that of suspension, the lineages in three population densities (PD): 10 5 , 10 4 and 10 3 CFU mL, at contact times (CT) of 15 and 30 minutes and from 1 up to 10 hours. Activity results were influenced by the PD and CT variables, with all yeasts inactivated. For example, at the highest PD: the standard strain was inactivated in the reading at 1 hr; of the clinical isolates, C. albicans was inactivated in the reading at 3 hrs; seven isolates of C. krusei at 30 minutes and the other 14 at 1 hr; seven isolates of C. rugosa were inactivated in the reading at 4 hrs, with the others inactivated at 10 hrs. Phytochemicals identified were phenolic compounds, tannins, condensed tannins and flavonoids. The evidence observed suggests the extract's potential for use on sources of infection in disinfection/antisepsis procedures, or in formulations which serve as a vehicle.

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“…and Cryptococcus spp. (Spanamberg et al, 2009). Antibiotic therapies have been the most common procedures in the treatment of mastitis in dairy cows.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of fixed oils found in Brazil nuts and sunflower seeds against microorganisms isolated from bovine subclinical mastitis

Freitas¹,

GonÃ§alves²,

Mendes³

et al. 2016

Afr. J. Microbiol. Res.

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Concern with traces of antibiotics found in milk and resistant bacteria has led to the search for alternatives, mainly natural products, to treat mastitis. This study aimed at evaluating the antimicrobial activity of fixed oils found in Brazil nuts and sunflower seeds against microorganisms isolated from mastitic milk and at analyzing their fatty acids. The following microorganisms were isolated from milk produced by cows with subclinical mastitis and selected for the antimicrobial test: Staphylococcus aureus, Enterococcus spp., Candida spp. and Cryptococcus laurentii. The oil components and the antimicrobial activity were determined by gas chromatography and by the broth microdilution technique, respectively. The following fatty acids were identified: myristic, palmitic, palmitoleic, margaric, stearic, oleic, linoleic, linolenic, arachidic, gadoleic, behenic and lignoceric. Averages of minimum inhibitory concentrations (MIC) for S. aureus, Enterococcus spp., Candida spp. and C. laurenti (sunflower seed oil), respectively. These values show that these oils, in low concentration, have antimicrobial activity against the microorganisms under study. Therefore, this study shows that these fixed oils may be used as therapeutic resources for the control of mastitis.

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Mastite micótica em ruminantes causada por leveduras

Abstract: RESUMO

Cited by 20 publications

References 34 publications

Aerobic fungi in the rumen fluid from dairy cattle fed different sources of forage

Aerobic fungi in the rumen fluid from dairy cattle fed different sources of forage

Disinfectant activity of the crude hydroalcoholic extract of Achyrocline satureioides (Asteraceae) on Candida spp. isolated in problem-situations of bovine mastitis

Antimicrobial activity of fixed oils found in Brazil nuts and sunflower seeds against microorganisms isolated from bovine subclinical mastitis

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