Avaliação de métodos de coleta de fezes para determinação da digestibilidade protéica em rã-touro (Rana catesbeiana)

Abstract

RESUMO O presente trabalho teve como objetivo avaliar diferentes metodologias de coleta de fezes para determinação da digestibilidade protéica de alimentos para a rã-touro (Rana catesbeiana). As metodologias estudadas foram o método

Fecal collection methods for the determination of protein digestibility in bullfrogs

“…These results differ from those reported by MOURIÑO & STÉFANI (2006) who observed significantly higher ADC CP for the decantation method when compared to intestinal dissection. This finding might be explained by the occurrence of nutrient leaching since the fecal samples in the decantation method were collected at intervals of 4h.…”

“…Digestibility assays have determined the metabolizable energy of some foods using the method of force-feeding and combined collection of total feces and water in the boxes in which the animals were kept (CASTRO et al, 2001;RODRIGUES et al, 2004). Within this context, MOURIÑO & STÉFANI (2006) evaluated four fecal collection methods for the determination of protein digestibility of bullfrog feeds and found that fecal decantation was the most indicated.…”

“…The intestinal dissection method can underestimate the results if the intestine is squeezed during feces removal, causing contamination with urine and intestinal mucosa (MOURIÑO & STÉFANI, 2006). The pressure applied during dissection may cause damage to the viscera and consequent fecal contamination with endogenous nitrogen from mucus and epithelial cells, reducing digestibility (HAJEN et al, 1993).…”

Métodos para determinação da digestibilidade aparente de dietas para camarão marinho suplementadas com probiótico

“…O sistema mais simples de coleta fecal é o sifonamento do material do fundo do tanque ou aquário. No entanto, esta técnica está sujeita a numerosos erros, entre eles a lixiviação dos nutrientes e da matéria seca para a água, o que pode superestimar os valores alcançados (Mouriño & De Stéfani, 2006).…”

Tracking viral particles in the intestinal contents of the American bullfrog, Lithobates catesbeianus, by Transmission Electron Microscopy

“…Due to the difficulty of collecting feces from living animals due to possible leaching of the feces by the water, tissue contamination and other substances from the animal itself, it was decided that the direct dissection of the large intestine would be performed (Mouriño and Stéfani, 2006). Before euthanizing the animals, they were anesthetized by hypothermia, inspected to detect any injury and subsequently desensitized and sacrificed through medullary sectioning and bleeding at the height of the atlas bone in the cervical region.…”