Crescimento de Acidovorax avenae subsp. citrulli sob diferentes temperaturas, pH, concentrações de cloreto de sódio e fontes de carbono

Cavalcanti, Márcia Tanajura; Silveira, Elineide Barbosa da; Mariano, Rosa de Lima Ramos; Viana, Ivanise O.

doi:10.1590/s0103-84782005000600013

Cited by 5 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Differences in the position of the leaf are strongly correlated with differences in the population of bacteria that colonize individual leaves over time (Kinkel et al, 2000). The reduction in the Ac1 Rif population on the 3 rd and 6 th pairs of leaves and consecutive stems (Figure 1) probably resulted from drastic alterations in temperature in the greenhouse, which reached approximately 41ºC, as the optimum temperature for the pathogen growth is 35ºC (Cavalcanti et al, 2005). When Ac1 Rif was inoculated in the seeds, there was colonization of the hypocotyl and roots, an increase in the population in the cotyledonary leaves and subsequent colonization of leaves and stems (Figure 2).…”

mentioning

confidence: 99%

Colonization dynamics of Acidovorax citrulli in melon

Alves¹,

Xavier²,

Viana³

et al. 2010

Trop. plant pathol.

Self Cite

View full text Add to dashboard Cite

The aim of this work was to investigate the ability of Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB) of cucurbits, to colonize melon tissues. Under greenhouse conditions leaves and seeds were inoculated with a spontaneous mutant of A. citrulli (group I) resistant to rifampicin (Ac1 Rif), and samples of hypocotyls, cotyledonary leaves, roots, leaves and stems were processed at three-day intervals. When the 1 st pair of melon leaves had been inoculated, A. citrulli was only detected until the 10 th pair of leaves (1.3 x 10 3 CFU g-1 of leaf) and the consecutive stem (3.3 x 10 3 CFU g-1 of stem) on the 30 th day. However, after seed inoculation A. citrulli colonized the hypocotyl, roots, cotyledonary leaves, leaves and stems and was only detected until the 4 th pair of leaves (1.62 x 10 2 CFU g-1 of leaf) and the consecutive stem (9.1 x 10 1 CFU g-1 of stem) on the 24 th day post-inoculation. In conclusion, A. citrulli colonized different parts of the melon plant over time, depending on its initial location, leaves or seed. This confirms what has been observed in the field, that expanded leaves and stems are the main inoculum sources for melon blossoms and fruit, therefore providing scientific bases for developing more effective strategies for BFB management.

show abstract

mentioning

confidence: 99%

Colonization dynamics of Acidovorax citrulli in melon

Alves¹,

Xavier²,

Viana³

et al. 2010

Trop. plant pathol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Regarding the remaining tests, the results were positive for oxidase, urease and growth at 41ºC. The strains used ethanol and D-glucose as carbon sources, but results were negative for mannitol, sucrose, arginine and sorbitol, differing from the results found by Cavalcanti et al (2005) for mannitol and sorbitol. These differences are probably due to variability that could be found within different groups of strains.…”

Section: Cultural Nutritional Physiological Serological and Molecumentioning

confidence: 82%

Comparing Acidovorax citrulli strains from melon and watermelon: Phenotypic characteristics, pathogenicity and genetic diversity

Melo¹,

Tebaldi²,

Mehta³

et al. 2014

Trop. plant pathol.

View full text Add to dashboard Cite

Melon and watermelon bacterial fruit blotch, incited by Acidovorax citrulli, is limited to some areas in Brazil but causes important losses, mainly in melon-producing regions. Although genetic diversity has been observed among strains belonging to the species, they are considered a homogeneous group based on the fact that they show only slight physiological or nutritional differences. The objective of this study was to compare Brazilian strains from melon and watermelon by means of biochemical, pathogenicity, serological and molecular assays. Fifteen biochemical tests, cross inoculation between strains and hosts, ELISA and repetitive sequence analysis (rep-PCR) with the primers REP, ERIC and BOX were conducted. No differences were revealed by nutritional characterization or serology, but cross inoculation showed different pathogenicity groups, which could explain high aggressiveness of the bacteria to melon crops in some regions. Molecular analysis by BOX-PCR clustered strains according to their geographical origin, while ERIC-and REP-PCR, analyzed together, indicated genetic diversity, but without geographical or host origin relationships. One test that could be used to verify the pathogenicity of strains by inoculating detached leaf petioles, showing results in 36 h, is proposed here.

show abstract

“…Acidovorax avenae subsp. citrulli requer pH próximo à neutralidade para maior crescimento (Cavalcanti et al, 2005).…”

Section: Crescimento De X Campestris Pv Viticola Em Meio De Culturaunclassified

Métodos de preservação e crescimento de Xanthomonas campestris pv. viticola em meio de cultura variando temperatura, pH e concentração de NaCl

2005

View full text Add to dashboard Cite

A bactéria fitopatogênica Xanthomonas campestris pv. viticola (Xcv) causa o cancro bacteriano da videira (Vitis vinifera), que ocasiona grandes prejuízos à viticultura no Brasil. Os métodos de dessecação em papel de filtro (DPF), repicagens periódicas (RP), água destilada esterilizada (ADE) e folhas herborizadas (FH) foram utilizados para preservar duas estirpes de Xcv durante 12 meses. As variáveis viabilidade e patogenicidade foram avaliadas mensalmente e estimadas pela obtenção de crescimento bacteriano e área abaixo da curva de incidência da doença (AACID). Tanto o método de DPF como o de ADE propiciaram viabilidade constante de 100% durante 11 meses e os maiores valores de AACID. No método de RP não houve crescimento das estirpes já aos 30 dias, enquanto que, em FH, Xcv foi isolada até cinco meses. o crescimento das estirpes de Xcv em meio de cultura líquido variando temperatura (0, 5, 10, 15, 20, 25, 27, 28, 29, 30, 35, 40 e 45 °C), pH (5,0; 5,5; 6,0; 6,5; 7,0; 7,5; 8,0; 8,5 e 9,0) e concentração de NaCl (1, 2, 3, 4, 5, 6 e 7%) foi avaliado em fotocolorímetro. O crescimento de Xcv foi observado no intervalo de 5 até 35 °C, enquanto que o crescimento ótimo ocorreu de 27 a 29 °C. A Xcv não cresceu a zero e 40 °C. O pH ótimo para o crescimento desta bactéria foi 7,5. O crescimento de Xcv decresceu a partir de 3,0% de NaCl, com concentração letal de 6,0%.

show abstract

Crescimento de Acidovorax avenae subsp. citrulli sob diferentes temperaturas, pH, concentrações de cloreto de sódio e fontes de carbono

Cited by 5 publications

References 6 publications

Colonization dynamics of Acidovorax citrulli in melon

Colonization dynamics of Acidovorax citrulli in melon

Comparing Acidovorax citrulli strains from melon and watermelon: Phenotypic characteristics, pathogenicity and genetic diversity

Métodos de preservação e crescimento de Xanthomonas campestris pv. viticola em meio de cultura variando temperatura, pH e concentração de NaCl

Contact Info

Product

Resources

About