Auto-enxerto percutâneo de medula óssea em coelhos: I. Coleta, preparo e aplicação

Barros, Séfora Vieira da Silva Gouvêa de; Carlo, Ricardo Junqueira Del; Vargas, Marlene; Galvão, Simone Rezende; Filho, Alfredo Maia

doi:10.1590/s0103-84782001000600016

Cited by 7 publications

(5 citation statements)

References 16 publications

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“…The importance of stem cells in bone regeneration and its relatively low quantity in relation to nucleated marrow cells have directed studies to cell separation, purification and concentration (Barros et al, 2001a). However, no culture, separation and/or differentiation of mesenchymal stem cells were performed, for the reasons mentioned above and to reduce risks of sample contamination during the process.…”

Section: Discussionmentioning

confidence: 99%

Bone regeneration in mandible defect with autograft bone and cell suspension from bone marrow in rabbits

Gomes

Paz

Bonilla

et al. 2011

Arq. Bras. Med. Vet. Zootec.

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The objective of this study was to investigate the bone regeneration of a "gold standard" (autograft) from iliac crest associated with cellular therapy in rabbits. A bone defect was created with 10x5x5mm in 28 rabbit mandibles. The control group animals (n=14) were repaired with autograft of iliac crest and the experimental group animals (n=14) received iliac crest autograft in association with mononuclear cells from the bone marrow of the femur. Weekly radiographs were taken of the surgery region and histological analyses was performed in seven animals in each group at 15 days and in seven animals of each group at 30 days after the surgery. A gradual increase of bone density was observed and the experimental animals presented the bone bridge in 85.7% (6/7) of the cases, while only 42.8% (3/7) of the animals in the control group presented this structure 28 days after the surgery. The histopathological parameters analyzed did not show any statistical difference between the control and experimental group in 15 and 30 days of analysis. The results suggest that the mononuclear cells from the marrow bone can better support the autograft regeneration in mandible defects in rabbits.Keywords: rabbit, mandibular reconstruction, bone regeneration, graft RESUMO Avaliou-se a regeneração óssea de auto-enxerto, considerado "padrão ouro" da crista ilíaca associado à terapia celular da medula óssea em coelhos. Foi criado um defeito ósseo de 10x5x5mm na mandíbula de

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Section: Discussionmentioning

confidence: 99%

Bone regeneration in mandible defect with autograft bone and cell suspension from bone marrow in rabbits

Gomes

Paz

Bonilla

et al. 2011

Arq. Bras. Med. Vet. Zootec.

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“…Barros et al (22) in 2001 treated failures of total segment of rabbits' radius with integral BM or BM centrifuged applied percutaneously. The percutaneous route application proved to be a simple method, with minimal trauma to the tissues, not introducing devitalized tissue and, thus, reducing the risk of infection and interference in bone repair.…”

Section: Stem Cells As Adjuvant In the Healing Of Fracturesmentioning

confidence: 99%

“…Barros et al (22) en 2011 trataron fallas de segmento total del radio de conejos con MO integral o MO centrifugada aplicadas por vía percutánea. La aplicación por vía percutánea se mostró un método sencillo, con mínimo trauma a los tejidos, no introduciendo tejido desvitalizado para de esta forma reducir el riesgo de infección y de interferencia en la reparación ósea.…”

Section: Stem Cells As Adjuvant In the Healing Of Fracturesunclassified

Células-madre adultas en la cicatrización de fracturas e injertos óseos

S¹,

Lpf

et al. 2018

Rev MVZ Córdoba

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The traumatic accidents which result in fractures and even in impairment of a bone segment have a high prevalence in routine veterinary surgery. Many times these fractures are complex and difficulty healing or need bone grafts to preserve and restore the length of the affected limb. Thus, the recovery time of these animals is usually delicate and long, with complications risks, such as delayed union and non-union of the fractured bone segments or absence of graft incorporation. In this sense, the stem cells (SC) have shown to be a strong ally of the orthopedic doctors due to their capacity to provide osteoprogenitor cells, to increase the osteoinductive effect minimize the healing time and provide a greater efficiency to the repairing process. Due to that, before the expectation of their assignments, this review aims to describe the stem cells characteristics, their current applications in the fractures healing and, especially, their contribution to the bone grafts consolidation.Keywords: Mesenchymal stem cells, bone tissue, cell therapy, bone transplantation (Source: MeSH). RESUMENLos accidentes traumáticos que resultan en fracturas y hasta en el comprometimiento de un segmento óseo tienen alta prevalencia en la rutina quirúrgica veterinaria. Muchas veces esas fracturas son complejas y presentan dificultades de cicatrización o necesitan de injertos óseos para preservar y restaurar el largo del miembro acometido. De esa forma, el tiempo de recuperación de esos animales suele ser delicado y prolongado con riegos de complicaciones, como unión retardada y no unión de los segmentos óseos fracturados o ausencia de incorporación de los injertos. En este sentido, las células-madre (CT) vienen demostrando ser una fuerte aliada de los ortopedistas debido a su capacidad en proveer células osteoprogenitoras, de incrementar el efecto osteoinductor, minimizar el tiempo de cicatrización y proporcionar mayor eficiencia al proceso reparador. Frente a la expectativa de sus atribuciones, la presente revisión tiene por objetivo describir las características de las células-madre, sus aplicaciones actuales en la cicatrización de fracturas y, especialmente, su contribución en la consolidación de injertos óseos.Palabras clave: Células-madre mesenquimales, tejido óseo, terapia celular, trasplante óseo (Fuente: MeSH). Adult stem cells in the healing of fractures and bone graftsCélulas-madre adultas en la cicatrización de fracturas e injertos óseos INTRODUCCIÓNEl principio de la terapia celular consiste en reparar tejidos u órganos dagnificados con la sustitución de las células no funcionales por células normales. Las CT presentes en el organismo, de forma natural, ejercen esa función; sin embargo, cuando las lesiones son más extensas, las CT no son capaces de corregirlas y la enfermedad se establece. En esas situaciones, la terapia celular con estas células tiene como objetivo potencializar el mecanismo natural de corrección, concentrando las mismas en el local de la lesión para que puedan actuar más eficientemente (1). Con los...

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“…Resultado que pode ter sido ocasionado pela falta de imobilização do membro submetido à intervenção cirúrgica no período pós-cirúrgico. Assim como devido à capacidade de indução osteogênica das células da medula óssea (BARROS et al, 2001). No GII-45 dias de PO foi verificado no coelho número dois, intensa reação periosteal, entretanto, não foi notado nos outros pertencentes também do GII-45 dias de PO (Figura 4).…”

Section: Animalunclassified

Aspectos Radiográficos Do Sulco Intertroclear De Coelhos Submetidos Ao Enxerto Osteocondral Alógeno Associado À Terapia Com Células Mononucleares Autógenas E Dexametasona

Faria

Eurides

Pereira

et al. 2016

ArqVet

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Gustavo Prudente Eurides 4FARIA, L. M.; EURIDES, D.; PEREIRA, D. P.; SOUZA, L. A. de; SILVA, L. V. P.; EURIDES, G. P. Aspectos radiográficos do sulco intertroclear de coelhos submetidos ao enxerto osteocondral alógeno associado à terapia com células mononucleares autógenas e desametasona. Arq. Ciênc. Vet. Zool. UNIPAR, Umuarama, v. 19, n. 2, p. 69-75, abr./jun. 2016. RESUMO:Vinte e quatro coelhos adultos foram separados em dois grupos (n=12) -controle (GI) e tratado (GII) e submetidos ao enxerto osteocondral alógeno para reparo ósseo e cartilaginoso do sulco troclear, conservado em glicerina a 98%. Os animais do GII receberam ainda injeção intra-articular de 2,0 x 10 6 células mononucleares autólogas e dexametasona intramuscular. Foram realizadas avalições radiográficas aos 45 e 90 dias de pós-operatório. Nos coelhos do grupo tratado e controle não foi notado sinais de reação características de enxerto-contra-hospedeiro e aos 45 e 90 dias de pós-operatório ocorre osteólise devido aos micromovimentos na interface implante-osso e pressão efetiva nos espaços articular. A implantação de enxerto alógeno conservado em glicerina, associado à inoculação de células mononucleares autógenas e dexametasona intramuscular promove intensa neoformação óssea e com bom reparo do defeito ósseo em coelhos. PALAVRAS-CHAVE: Implante. Radiografia. Reparação óssea. Terapia cellular. RADIOGRAPHIC APPEARANCE OF INTERTROCHLEAR GROOVE OF RABBITS SUBMITTED TO OSTEOCHONDRAL GRAFT ALLOGRAPHS ASSOCIATED TO THE THERAPY WITH MONONUCLEAR CELL AUTOGRAPHS AND DEXAMETHASONEABSTRACT: Twenty-four adult rabbits were divided two groups (n = 12), control (GI) and treated (GII), and submitted to allogenic osteochondral graft for bone and cartilage repair of the trochlear groove, preserved in 98% glycerin. The GII yet received intra-articular injection of 2,0 x 10 6 autologous mononuclear cells and intramuscular dexamethasone. Radiographs at 45 and 90 days postoperatively avaliations were performed. In rabbits treated and control group was not skimmed signals characteristic reaction of graft-versus-host and at 45 and 90 days postoperatively osteolysis occurs due to micro motion at the implant-bone interface and effective pressure in the joint spaces. The implantation of allograft preserved in glycerol, associated with inoculation of autologous mononuclear cells and intramuscular dexamethasone intense bone and good repair of bone defects in rabbits neogenesis occurs. KEYWORDS: Bone repair. Cell therapy. Implantation. Radiography. ASPECTOS RADIOLÓGICOS DE LA RANURA INTERTROCLEAR DE CONEJOS SOMETIDOS A INJERTO OSTEOCONDRAL ALÓGENO ASOCIADO A LA TERAPIA CON CÉLULAS MONONUCLEARES AUTÓGENAS Y DEXAMETASONA RESUMEN:Veinticuatro conejos adultos fueron divididos en dos grupos (n = 12) -control (GI) y tratado (GII) y sometidos a injerto osteocondral alógeno para reparo óseo y cartilaginoso de ranura tróclea, conservado en glicerina a 98%. Los animales del GII recibieron inyección interarticular de 2,0 x 10 6 células mononucleares autólogas y dexametasona intramuscula...

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Auto-enxerto percutâneo de medula óssea em coelhos: I. Coleta, preparo e aplicação

Cited by 7 publications

References 16 publications

Bone regeneration in mandible defect with autograft bone and cell suspension from bone marrow in rabbits

Bone regeneration in mandible defect with autograft bone and cell suspension from bone marrow in rabbits

Células-madre adultas en la cicatrización de fracturas e injertos óseos

Aspectos Radiográficos Do Sulco Intertroclear De Coelhos Submetidos Ao Enxerto Osteocondral Alógeno Associado À Terapia Com Células Mononucleares Autógenas E Dexametasona

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