Avaliação histológica do processo de reparação da superfície articular de coelhos

Souza, Tamara Daiane de; Carlo, Ricardo Junqueira Del; Vilória, Marlene Isabel Vargas

doi:10.1590/s0103-84782000000300011

Cited by 5 publications

(7 citation statements)

References 7 publications

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“…As cellular differentiation occurs, the repair tissue shows frequent fibrocartilage aspect and, at the deepest repair tissue region, new subchondral bone formation occurs. Such findings are in agreement with reports of Mitchel and Shepard (1976); Hurtig et al (1988);French et al (1989); Shamis et al (1989); Kim et al (1991), Shapiro et al (1993); Souza et al (1999); Souza et al (2000), who still mention that tissue architecture regeneration of articular surface is a rare event, occurring in a reduced number of osteochondral defects, a fact confirmed in this study, as there was no hyaline architecture restoration in any of the 48 animals used.…”

Section: Discussionsupporting

confidence: 91%

“…The macroscopic findings of osteochondral defects from both groups at the end of T15 are in agreement with the report of Souza et al (2000), who noticed partial filling of defect by reddishpinkish repair tissue at 15 days after injury induction in the trochlear sulcus in rabbits. At 30 postoperative days (T30), partial filling by white-color tissue did not correspond to that found by Hurtig et al (1988), who reported that, when similar injuries were induced in the wrist of equines, there were few alterations at 30 postoperative days.…”

Section: Discussionsupporting

confidence: 91%

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Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings

Eleotério

Pontes²,

Machado³

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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Chondroitin and glucosamine sulfate nutraceuticals are commonly used in the management of degenerative articular disease in veterinary routine. However, there are controversies on the contribution of these substances to articular cartilage. The purpose of this study was to evaluate the efficiency of a chondroitin and glucosamine sulfate-based veterinary nutraceutical on the repair of an induced osteochondral defect in a dog femoral condyle, by macroscopic, histological and histomorphometric analyses. The nutraceutical was orally administered the day following injury induction, every 24 hours (treated group, TG, n=24), compared with animals that did not receive the product (control group, CG, n=24). Six animals per group were anaesthetized for sample collection at 15, 30, 60 and 90 days after surgery. At 15 days, defects were macroscopically filled with red-pinkish tissue. After 30 days, whitish color tissue was observed, both in TG and CG animals, with firmer consistency to touch at 60 and 90 postoperative days. Histological analysis demonstrated that, in both groups, there was initial blood clot formation, which was subsequently substituted by a fibrin net, with capillary proliferation from the adjacent bone marrow and infiltration of mesenchymal cells in clot periphery. As cellular differentiation developed, repair tissue presented a fibrocartilage aspect most of the time, and new subchondral bone formation occurred in the deepest area corresponding to the defect. Histomorphometry suggested that the nutraceutical did not favor the articular cartilage repair process. It was concluded that nutraceutical did not significantly influence chondrocytes proliferation or hyaline architecture restoration.Keywords: degenerative articular disease, osteoarthritis, arthrosis, chondroprotective, cartilage RESUMO Os nutracêuticos compostos de sulfato de condroitina e glucosamina são comumente utilizados no manejo da doença articular degenerativa na rotina veterinária. Entretanto, existem controvérsias sobre a contribuição dessas substâncias à cartilagem articular. O objetivo deste trabalho foi avaliar a eficácia de um nutracêutico veterinário à base de sulfato de condroitina e glucosamina na reparação de defeitos osteocondrais induzidos no côndilo femoral de cães, através de análises macroscópica, histológica e histomorfométrica. O nutracêutico foi administrado no dia seguinte à indução da lesão, pela via oral, a cada 24 horas (grupo tratado -GT, 24 animais), sendo comparado a animais que não receberam o produto (grupo controle -GC, de igual número de animais

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings

Eleotério

Pontes²,

Machado³

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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show abstract

“…Chondrocytes isolated from non-articular cartilage contain larger amounts of lipid and glycogen inclusions due to the slower metabolism of this tissue [20,21]. In addition, studies have shown differences in the structure of ECM molecules isolated from cartilage obtained from anatomical sites that withstand compressive loads and those that do not [22].…”

Section: Introductionmentioning

confidence: 99%

Effects of microcurrent stimulation on Hyaline cartilage repair in immature male rats (Rattus norvegicus)

Ciccone

Zuzzi²,

Neves³

et al. 2013

BMC Complement Altern Med

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BackgroundIn this study, we investigate the effects of microcurrent stimulation on the repair process of xiphoid cartilage in 45-days-old rats.MethodsTwenty male rats were divided into a control group and a treated group. A 3-mm defect was then created with a punch in anesthetized animals. In the treated group, animals were submitted to daily applications of a biphasic square pulse microgalvanic continuous electrical current during 5 min. In each application, it was used a frequency of 0.3 Hz and intensity of 20 μA. The animals were sacrificed at 7, 21 and 35 days after injury for structural analysis.ResultsBasophilia increased gradually in control animals during the experimental period. In treated animals, newly formed cartilage was observed on days 21 and 35. No statistically significant differences in birefringent collagen fibers were seen between groups at any of the time points. Treated animals presented a statistically larger number of chondroblasts. Calcification points were observed in treated animals on day 35. Ultrastructural analysis revealed differences in cell and matrix characteristics between the two groups. Chondrocyte-like cells were seen in control animals only after 35 days, whereas they were present in treated animals as early as by day 21. The number of cuprolinic blue-stained proteoglycans was statistically higher in treated animals on days 21 and 35.ConclusionWe conclude that microcurrent stimulation accelerates the cartilage repair in non-articular site from prepuberal animals.

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“…O coelho é um animal amplamente utilizado em modelos para reparação da superfície articular (RIBEIRO, CAMANHO e TAKITA, 2004;Souza et al, 2000;O´Driscoll et al, 1988;Mow et al, 1991;Costa et al, 2001;Rudert et al, 2005;Hasegawa et al, 1999;Shahgaldi et al, 1998;Diduch et al, 2000;Mierish et al, 2002;Cohen et al, 2003;Frenkel et al, 2005;Uematsu et al, 2005;Shao et al, 2006;Witte et al, 2006;Suominen et al, 2006;Ohyabu et al, 2008). Porém, na literatura encontram-se diversos trabalhos com diferentes modelos animais como cães (Welsing et al, 2008), cabras (Custers et al, 2009), porcos (Chiang et al, 2005;Vassara et al, 2006), cavalos (Sams e Nixon, 1995), ovelhas (Erggelet et al, 2009), ratos (Kondo et al, 2005), carneiros (Kandel et al, 2006) e bugios (Malinin et al, 2006).…”

Section: Discussionunclassified

“…Na literatura consultada, constatou-se que tanto a região dos côndilos femorais (Mitchell e Sheperd, 1976;Inouye et al, 2002;Sakihama et al, 2006;RIBEIRO, CAMANHO e TAKITA, 2004;O´Driscoll et al, 1988;Mow et al, 1991;Messner e Gillquist, 1993;Diduch et al, 2000;Frenkel et al, 2005) como a região do sulco troclear Souza et al, 2000;Rudert et al, 2005;Hasegawa et al, 1999;Shahgaldi et al, 1998;Cohen et al, 2003;Uematsu et al, 2005;Witte et al, 2006;Suominen et al, 2006;Kandel et al, 2006) foram sede para estudos sobre reparo de cartilagem.…”

Section: Discussionunclassified

Desenvolvimento de um biomaterial composto de poliuretano e microfibra de biovidro 45S5 em gradiente funcional para reparo de cartilagem articular: estudos in vitro e in vivo

Laurenti¹

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Agradeço a Deus pelos privilégios de minha vida. Ao meu querido orientador Dr. Carlos Alberto Fortulan por ter sido a "minha bússola" na realização deste trabalho. O modo como me apoiou no decorrer deste trabalho: uma orientação científica criteriosa, estimulando e dando o tempo para uma construção pessoal do trabalho. A disponibilidade que sempre manifestou e a empatia com que recebeu as minhas idéias foram o estímulo que me permitiu vencer as inseguranças deste processo, e, principalmente pela orientação, condução, participação durante as fases desta pesquisa, paciência e boa vontade em ensinar e principalmente pela amizade. Para mim, é uma imensa honra e orgulho tê-lo como meu orientador. Ao Dr. João Manuel Domingos de Almeida Rollo ter como meu co-orientador foi um privilégio. De coração, sou grata pela disponibilidade, dedicação, conselhos e atenção prestadas para a realização desse trabalho. Ao prof. Titular Benedito de Moraes Purquerio pela oportunidade. Ao Dr. Arnaldo Rodrigues Santos Júnior pelo auxílio na realização dos testes in vitro. E principalmente por ter contribuído com importantes e enriquecedoras sugestões que com certeza foram imprescindíveis para a realização deste trabalho. E por sua disposição em me ajudar... SEMPRE. Ao Dr. Rodrigo Bezerra de Menezes Reiff, cirurgião ortopedista brilhante, agradeço a parceria, disposição, boa vontade e participação neste projeto. A Dra. Ana Maria Minarelli Gaspar, eu sempre pude contar com você! Tenho certeza de que somei à minha lista de amigos mais um nome de peso. A Profa. Dra. Eliana Cristina da Silva Rigo "Lica" a minha melhor escolha para participar da minha banca. Obrigada pelas sugestões e boa vontade. Ao técnico Pedrinho da Histologia da FOAr-Unesp. Aos amigos do Laboratório de Tribologia e Compósitos. À querida "fotógrafa" e amiga Lourdes Cristina de Albuquerque Haach por sua ajuda e a Camila Roberta Meira. A Luciana Portal e a Lubrizol pela doação do poliuretano médico. A Ted Day da Mo-Sci Corporation pela atenção dispensada e pela doação da microfibra de biovidro 45S5.

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Avaliação histológica do processo de reparação da superfície articular de coelhos

Cited by 5 publications

References 7 publications

Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings

Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings

Effects of microcurrent stimulation on Hyaline cartilage repair in immature male rats (Rattus norvegicus)

Desenvolvimento de um biomaterial composto de poliuretano e microfibra de biovidro 45S5 em gradiente funcional para reparo de cartilagem articular: estudos in vitro e in vivo

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