Clinical aspects of experimental peritonitis in horses

Abstract

Sixteen adult horses were randomly divided into 4 equal groups (GI, GII, GIII and GIV) of 4 animals and each group was injected intraperitoneally with one of the following suspension: GI (100 x 10(7) colony-forming units (CFU) of Escherichia coli diluted in 500ml of 0.9% saline); GII (100 x 10(7) CFU of Bacteroides fragilis in 500ml of 0.9% saline); GIII (100 x 10(7) CFU of E. coli in combination with 100 x 10(7) CFU of B. fragilis in 500ml of 0.9% saline); GIV (500ml of 0.9% saline). Abdominal wall sensitivity to external pressure and tension, diarrhea, decreased intestinal sounds and increased of heart rate were the clinical signs more frequently observed in inoculated horses. Horses inoculated with pure cultures of either E. coli or B. fragilis demonstrated mild and self-limiting peritonitis, while those inoculated with the combination of both bacteria demonstrated clinical signs of higher intensity and duration.

Effects of Lidocaine Infusion during Experimental Endotoxemia in Horses

“…Also, the appearance of band neutrophils and apoptotic or dead cells has been demonstrated in a porcine endotoxemia model 45 . In contrast, the increase in the number of nucleated cells, PMN and MN cells, fibrinogen concentration, and total protein concentration in PF after IP injection of LPS in both groups characterizes acute peritoneal inflammation 21,46,47 …”

“…Migration of PMN and MN cells into the abdominal cavity may be a protective response against intact bacteria or LPS spillover into this compartment during endotoxemia. In addition, serial PF collection is not a sufficient stimulus to evoke a high migration of PMN cells into the abdominal cavity 21,47,55 …”