Citogenética em cinco espécies ornamentais de liliales

Lovatto, Marlene Terezinha; Battistin, Alice

doi:10.1590/s0103-84781997000400010

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…Plant cytogenetic studies in Brazil are focused on cultivated plants with an economic interest; however, interest has developed on other species, especially of medicinal and ornamental value (Guerra 1990;Lovatto and Battistin 1997;Pedrosa et al 1999;Pagliarini 2000). Cytogenetic analyses can be used as a marker in multifaceted taxonomy tendency.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic characterization ofSchinus terebinthifoliusRaddi (Anacardiaceae) accessions from Rio Grande do Sul State, Brazil

et al. 2015

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Schinus terebinthifolius (Anacardiaceae), popularly known as red aroeira, occurs naturally in Argentina, Paraguay, Uruguay, and Brazil. It has medicinal properties and is one of the species most sought by avifauna, so it is useful in heterogeneous reforestation intended for the recomposition of degraded areas of permanent preservation. Information about chromosomes, from simple chromosome counting to detailed molecular cytogenetics, is relevant in systematic and evolutionary studies. This study aimed to perform chromosomal characterization of 22 accessions of S. terebinthifolius native to Rio Grande do Sul, aiming to verify the intraspecific variability in this species, besides the conservation and sustainable use of this species. For the determination of chromosome number, tissues of root tips were subjected to a cold pretreatment (± 4°C) for 18 h. Afterwards, the roots were fixed in Carnoy 3:1 (ethanol/acetic acid) for 24 h at room temperature and stored under alcohol (70%) under cooling. The slides were prepared by the squashing technique and stained with 2% acetic orcein. The chromosome number of S. terebinthifolius found in 22 accessions was 2n = 28, and these studied accessions do not exhibit intraspecific variability.

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Section: Introductionmentioning

confidence: 99%

Cytogenetic characterization ofSchinus terebinthifoliusRaddi (Anacardiaceae) accessions from Rio Grande do Sul State, Brazil

et al. 2015

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“…Nestas regiões os genes transcricionais estão ativos, caracterizando cada uma das espécies, porém podem ocorrer sítios não ativos/silenciosos, principalmente em espécies híbridas, que não são detectados pela técnica AgNOR, necessitando aplicação da técnica de rDNA-FISH. O número e o tamanho dos nucléolos, geralmente indicam a quantidade de genes ribossomais ativos (Lovatto & Battistin, 1997;Battistin et al 1999;Sumner, 2003).…”

Section: Introductionunclassified

Caracterização citogenética para identificação dos níveis de ploidia em cinco espécies do gênero Mentha L.

Battistin

Almeida

Nogueira

et al. 2013

Rev. bras. plantas med.

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RESUMO:Neste trabalho foi feita a caracterização citogenética da: microsporogênese, tétrades, estimativa da viabilidade do pólen pelo método de coloração e contagem do número máximo de nucléolos por célula interfásica, para identificação dos níveis de ploidia, em cinco espécies do gênero Mentha L. Foram coletadas inflorescências em 30 plantas de cada espécie, em duas florações sucessivas, nos anos 2006 e 2007. As inflorescências foram tratadas em etanol-ácido acético (3:1), em temperatura ambiente durante seis horas, transferidas para álcool 70% (v/v) e conservadas em geladeira até análise. Nas análises da microsporogênese, tétrades e pólen o corante usado foi carmin propiônico 2% e na identificação dos nucléolos nitrato de prata (AgNO 3 ). Os resultados demonstraram que as cinco espécies são poliplóides. M. crispa heptaplóide (2n=7x=84) com 11 nucléolos, M. spicata tetraplóide (2n=4x=48) com 8 nucléolos, M.x gentilis pentaplóide (2n=5x=60) com 12 nucleólos, M. piperita e M.x piperita ambas hexaplóides (2n=6x=72) com 8 e 9 nucléolos respectivamente. M. spicata e M. crispa mantiveram as mais altas porcentagens de células normais na microsporogênese, na formação de tétrades e na estimativa da viabilidade do pólen por coloração, sugerindo maior estabilidade meiótica quando comparados aos demais poliplóides estudados.Palavras-chave: microsporogênese, cromossomos, poliploidia, Mentha.ABSTRACT: Citogenetic characterization to determination of ploidy level on five Mentha L. species . The cytogenetic characterization of five species of Mentha L. genus, including the data: regularity of microsporogenesis and tetrads, and polen viability, using the coloration method and the counting of the maximum number of nucleolus by interphasic cell were carried out in this study to identify the ploid levels. These analyses were performed from inflorescences collected in 30 plants of each species, during two successive florations in 2006 and 2007. Inflorescences were treated in 3:1 ethanol:acetic acid mixture at room temperature during six hours, then transferred to 70%(v/v) ethanol solution and refrigerated until the analysis. For microsporogenis, tetrad and pollen analysis, we used carmine propionic 2% (m/v) and for nucleolus identification, we used AgNO 3 solution. It was possible to observe that all five species were polyploids. M. crispa heptaploid (2n=7x=84) with 11 nucleolus, M. spicata tetraploid (2n=4x=48) with 8 nucleolus, M. x gentilis pentaploid (2n=5x=60) with 12 nucleolus, M. piperita and M. x piperita both hexaploid (2n=6x=72) with 8 and 9 nucleolus respectively. M. spicata and M. crispa kept the highest percentual values of normal cells in microsporogenesis as well as in tetrads formation and pollen viability, suggesting a higher meiotic stability when compared to the other polyploids studied.

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Variability of the chromosomal number and meiotic behavior in populations ofBidens pilosaL. (Asteraceae) from southern Brazil

et al. 2008

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Citogenética em cinco espécies ornamentais de liliales

Cited by 4 publications

References 12 publications

Cytogenetic characterization ofSchinus terebinthifoliusRaddi (Anacardiaceae) accessions from Rio Grande do Sul State, Brazil

Cytogenetic characterization ofSchinus terebinthifoliusRaddi (Anacardiaceae) accessions from Rio Grande do Sul State, Brazil

Caracterização citogenética para identificação dos níveis de ploidia em cinco espécies do gênero Mentha L.

Variability of the chromosomal number and meiotic behavior in populations ofBidens pilosaL. (Asteraceae) from southern Brazil

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