Detection of Bovine Respiratory Syncytial Virus in Calves of Rio Grande Do Sul, Brazil

Gonçalves, Ivan Paulo Demartini; Simanke, Agda Theisen; Jost, Hardy; Hötzel, Isidro; Soglio, Adriana Dal; Moojen, Valeria

doi:10.1590/s0103-84781993000300029

Cited by 15 publications

(12 citation statements)

References 3 publications

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“…Recent studies have demonstrated the presence of BRSV antibodies in numerous serum samples, as well as viral antigens in histological specimens used for the diagnosis of bovine infec- tions in the south and southeast regions of the country (6)(7)(8)(9). A serological investigation was performed with 864 serum samples from 65 different farms located in the southern Brazilian states, using serum neutralization test (SNT) and enzyme-linked immunosorbent assay (ELISA), and the clinical history was analyzed.…”

Section: Bovine Respiratory Syncytial Virus (Brsv)mentioning

confidence: 99%

“…In former studies with samples from autopsied animal tissues, the difficulty in obtaining these free and infective viruses seemed to be greater due to the time elapsed between death, autopsy and inoculation in the cell cultures (6,9). Furthermore, it is easier to obtain tissue samples from animals autopsied during the acute phase of the disease (10).…”

Section: -Brmentioning

confidence: 99%

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Characterization of bovine respiratory syncytial virus isolated in Brazil

Arns

Campalans

Costa

et al. 2003

Braz J Med Biol Res

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This paper presents the first isolation of bovine respiratory syncytial virus in Brazil and its physicochemical, morphological and molecular characterization. The virus was isolated from 33 samples of nasotracheal secretions, successively inoculated into a Madin-Darby bovine kidney cell culture, which was characterized by physicochemical tests and morphological observation by electron microscopy. The Brazilian sample is an RNA pleomorphic, enveloped, thermolabile and nonhemagglutinating spicular virus. Reverse transcription, followed by nested polymerase chain reaction (nRT-PCR) assay was carried out using oligonucleotides B1, B2A, B3 and B4 for the fusion proteins (F) and B5A, B6A, B7A and B8 for the attachment protein (G). The nRT-PCR-F amplified a fragment of 481 bp corresponding to part of the gene that codes for protein F, whereas nRT-PCR-G amplified a fragment of 371 bp, in agreement with part of the G gene. The virus isolated from Brazilian samples in this study corresponded to the bovine respiratory syncytial virus, and RT-PCR proved to be useful for the diagnosis of bovine clinical samples.

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Section: Bovine Respiratory Syncytial Virus (Brsv)mentioning

confidence: 99%

Section: -Brmentioning

confidence: 99%

Characterization of bovine respiratory syncytial virus isolated in Brazil

Arns

Campalans

Costa

et al. 2003

Braz J Med Biol Res

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“…Very little is known about the significance of BRSV in Brazil. Recent studies have shown BRSV antibodies in numerous serum samples and BRSV specific antigens have been detected in histological specimens of bovines submitted to veterinary diagnosis in the southern and southeastern regions of Brazil [4,9,11,13].…”

Section: Introductionmentioning

confidence: 99%

Dot-enzyme linked immunosorbent assay as an alternative technique for the detection of bovine respiratory syncytial virus (BRSV) antibodies

et al. 2002

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-A dot-ELISA test for the detection of anti-BRSV antibodies is described. The objective of this study was the standardisation of a test as a fast, inexpensive and effective alternative to detect anti-BRSV antibodies. Its sensitivity, specificity and usefulness were compared to a commercial ELISA-kit and to the standard serum neutralisation (SN) test. The standardisation of the technique was done using nitrocellulose disks soaked with a viral sample isolated in Brazil, BRSV-25-BR. The best results were obtained when the disks were sensitised with a purified antigen at a concentration of 0.7 µg/disk and the bovine serum was diluted 1: 200. The experiment used 423 samples of bovine serum collected in the main cattle breeding centres in Brazil.

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“…Em bezerros, o BRSV foi identificado desde 1967; seu primeiro isolamento foi feito na Suécia por Paccaud & Jacquier (1970). No Brasil, o primeiro registro da presença do vírus foi feita por Gonçalves et al (1993), através de imunoflorescência e cultivo celular de amostras de tecido pulmonar de bezerros, coletadas em frigoríficos na Grande Porto Alegre, RS. Campalans & Arns (1995) Fragmentos de pulmão foram colhidos em formalina a 10%, processados pelo método usual, cortados à espessura de 5µm e corados pela hematoxilina-eosina.…”

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Infecção natural pelo Vírus Sincicial Respiratório Bovino (BRSV) no Estado de Alagoas

et al. 2000

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RESUMO.-Descreve-se a ocorrência de infecção pelo vírus sincicial respiratório bovino (BRSV) em bezerros descendentes de animais das raças pardo-suíça e holandesa importados da Alemanha, Áustria, Suíça e Uruguai, na qual morreram em Alagoas, Brasil, pelo menos 220 cabeças, de 1995 até a presente data. O quadro clínico caracterizou-se por hipertermia, tosse seca, mais tarde dispnéia acentuada e por vezes lacrimejamento; à auscultação havia estertores secos, depois úmi-dos, com sibilos, muitas vezes audíveis à distância. O exame histológico revelou pneumonia intersticial com formação de células sinciciais, infiltração predominantemente linfocitária com presença de eosinófilos e de corpúsculos de Russel, proliferação de pneumócitos tipo II e leve metaplasia escamosa. Células epiteliais de bronquíolos e células sinciciais marcaram-se positivamente com o anticorpo anti-BRSV. A ocorrên-cia da enfermidade no Sul e agora no Nordeste do Brasil indica a necessidade de se promover um amplo levantamento epidemiológico para se avaliar o grau de perdas e a proporção de animais infectados no país. Lembramos que parte dos animais importados, ao que tudo indica, já estavam infectados nos países de origem, quando desembarcaram em Belém, Pará. Cases of bovine respiratory syncytial virus (BRSV) infection affecting calves in the State of Alagoas, Brazil, are described. At least 220 calves, which were the progeny of Brown Swiss and Holstein Friesian cattle imported from Germany, Austria, and Uruguay, have died from the disease since 1995. Clinical signs included fever, dry cough, serous ocular discharge and, towards the final stages, marked dyspnea. On auscultation there were loud and harsh breathing

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Detection of Bovine Respiratory Syncytial Virus in Calves of Rio Grande Do Sul, Brazil

Cited by 15 publications

References 3 publications

Characterization of bovine respiratory syncytial virus isolated in Brazil

Characterization of bovine respiratory syncytial virus isolated in Brazil

Dot-enzyme linked immunosorbent assay as an alternative technique for the detection of bovine respiratory syncytial virus (BRSV) antibodies

Infecção natural pelo Vírus Sincicial Respiratório Bovino (BRSV) no Estado de Alagoas

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