Dot-enzyme linked immunosorbent assay as an alternative technique for the detection of bovine respiratory syncytial virus (BRSV) antibodies

Abstract

-A dot-ELISA test for the detection of anti-BRSV antibodies is described. The objective of this study was the standardisation of a test as a fast, inexpensive and effective alternative to detect anti-BRSV antibodies. Its sensitivity, specificity and usefulness were compared to a commercial ELISA-kit and to the standard serum neutralisation (SN) test. The standardisation of the technique was done using nitrocellulose disks soaked with a viral sample isolated in Brazil, BRSV-25-BR. The best results were obtained when the disks were sensitised with a purified antigen at a concentration of 0.7 µg/disk and the bovine serum was diluted 1: 200. The experiment used 423 samples of bovine serum collected in the main cattle breeding centres in Brazil.

Generation and characterization of monoclonal antibodies against the flounder Paralichthys olivaceus rhabdovirus

“…It is known that immunodot test is based on the same principles than the Western blot. The difference between both assays is that there is no need for the former to transfer the antigens to the PVDF membrane with electrophoresis, so it requires little amounts of antibodies, antigens and it also permits the use of non-purified antigens (Domingues et al, 2002). Hence, with 1 l PORV-infected GCO cell supernatants, the immunodot analysis can give positive signals.…”

Atividade Viral Do Vírus Respiratório Sincicial Bovino (Brsv) Em Bezerros Leiteiros

“…Em nosso país, apesar de alguns estudos determinarem a ocorrência do BRSV nos rebanhos por meio de testes sorológicos (DOMINGUES et al, 2002), pouco se sabe sobre a epidemiologia do vírus no Brasil, e ainda não existem pesquisas relacionadas ao monitoramento de títulos de anticorpos em bezerros até um ano de idade. Devido a isso, este estudo foi realizado com o objetivo de determinar a ocorrência da atividade viral em três rebanhos leiteiros soropositivos para o BRSV da região noroeste do Estado de São Paulo, por meio da análise do soro sanguíneo de bezerros, desde o primeiro dia de vida até os animais completarem 12 meses.…”