Cytotoxic effects of White-MTA and MTA-Bio cements on odontoblast-like cells (MDPC-23)

Abstract

This study evaluated the cytotoxic effects of 2 mineral trioxide aggregate (MTA) cements - White-MTA-Angelus and a new formulation, MTA-Bio - on odontoblast-like cell (MDPC-23) cultures. Twenty-four disc-shaped (2 mm diameter x 2 mm thick) specimens were fabricated from each material and immersed individually in wells containing 1 mL of DMEM culture medium for either 24 h or 7 days to obtain extracts, giving rise to 4 groups of 12 specimens each: G1 - White-MTA/24 h; G2 - White-MTA/7 days; G3 - MTA-Bio/24 h; and G4 - MTA-Bio/7 days. Plain culture medium (DMEM) was used as a negative control (G5). Cells at 30,000 cells/cm(2) concentration were seeded in the wells of 24-well plates and incubated in a humidified incubator with 5% CO2 and 95% air at 37 degrees C for 72 h. After this period, the culture medium of each well was replaced by 1 mL of extract (or plain DMEM in the control group) and the cells were incubated for additional 2 h. Cell metabolism was evaluated by the MTT assay and the data were analyzed statistically by ANOVA and Tukey's test (alpha=0.05). Cell morphology and the surface of representative MTA specimens of each group were examined by scanning electron microscopy. There was no statistically significant difference (p>0.05) between G1 and G2 or between G3 and G4. No significant difference (p>0.05) was found between the experimental and control groups either. Similar cell organization and morphology were observed in all groups, regardless of the storage periods. However, the number of cells observed in the experimental groups decreased compared to the control group. MTA-Bio presented irregular surface with more porosities than White-MTA. In conclusion, White-MTA and MTA-Bio presented low cytotoxic effects on odontoblast-like cell (MDPC-23) cultures.

Novel experimental cements for use on the dentin-pulp complex

“…The relatively poor viability seen with MTA compared with the control (49%) is suggestive of at least an initial toxic effect of MTA. While some of the previously published studies have reported higher cell viability in the presence of MTA (16,17), the data obtained during the first 24 h post-setting perhaps reflected the high pH generated by the material during this period (11). The pH values for MTA of around 12.0 have been reported in other studies (18,19).…”

Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells

“…In spite of the critical role of calcium ions in the tissue repair process and also its combination with water leading to the setting expantion of material such as MTA and CEM, a high release of these ions in the culture medium may result in irreversible damage to the cell culture 41) . It should be emphasized that the results obtained from this preliminary genotoxicity test had limitations therefore further studies employing CEM are required to demonstrate its genotoxicity in different methods of evaluation such as LDH, analysis of qRT-PCR, micronucleus, necrosis and apoptosis assay by FACS, confocal laser microscope.…”

Histologic Evaluation of Furcation Perforation Treated with Mineral Trioxide Aggregate and Bioaggregate

“…More recently, new products similar to MTA have been introduced including MTA Angelus (Hashem and Hassanien, 2008), MTA-Bio (Lessa et al, 2010) and BioAggregate (Zhang et al, 2009). BioAggregate is a relatively new bioceramic based material which is similar to MTA.…”