Copaiba oil effect under different pathways in mice subjected to sepsis

Abstract

PURPOSE:To evaluate the effects of copaiba oil administered by different routes on survival of mices subjected to cecal ligation and puncture. METHODS:Thirty two mice were distributed into four study groups (N=8): Sham group: normal standard animals; Control group: submitted a cecal ligation and puncture (CLP); Gavage group: submitted a CLP, and treat with copaiba oil by gavage; and Subcutaneous group: submitted a CLP, and treat with copaiba oil by subcutaneous injection. After the death of the histological analysis were performed.The Kaplan-Meier curves of surviving time were realized. RESULTS:All animals that received copaiba, regardless of the route used, survived longer when compared to the control group (p<0.0001), whereas the survival time ranged from 20 hours for the control group up to 32 hours for the animals of gavage group and 52 for subcutaneous group. The animals that received gavage copaiba lived about and about 20 hours unless the subcutaneous group (p=0.0042). There was no statistical difference when compared the intensity of inflammatory response (p>0.05) CONCLUSION: Prophylactic subcutaneous administration of copaiba in mice subjected to severe sepsis by cecal ligation and puncture, resulted in a survival time higher than non-use or use of this oil by gavage.

Effects of cococonut water and simvastatin in the treatment of sepsis and hemorrhagic shock in rats

“…In the present study, we compared the effects 0.9% saline, modified coconut water (Na 3%) and water coconut 3% + Na + simvastatin, and normal coconut water on serum cytokines, biochemical and white blood cell count in rodent model with hemorrhagic shock and abdominal sepsis. Experimental models of sepsis and hemorrhagic shock has often been employed in other estudies [30][31][32] . In the present study, we found that treatment of rats with sepsis and shock with coconut water modified with sodium 3% + simvastatin reduced serum cytokines, biochemical parameters and peripheral leukocytes.…”

Aloe vera delays mortality, but does not attenuate kidney injury in septic mices

“…The CLP model of sepsis has been a mainstay of basic sepsis research; Some medicinal plants have been tested in that model [9,[18][19][20][21], most with encouraging result; however, the aloe has been used therapeutically for many centuries and has the advantage of clinical studies demonstrating its safety and effectiveness in several diseases [22][23][24], facilitating the translation. However, more studies are necessary to assess variables of drug administration such as: the best routes of administration (oral, subcutaneous, intraperitoneal or intravenous) and dose and frequency of administration for better results [18,19].…”

“…However, more studies are necessary to assess variables of drug administration such as: the best routes of administration (oral, subcutaneous, intraperitoneal or intravenous) and dose and frequency of administration for better results [18,19]. …”

Effects of Veronica officinalis extract in the kidney of rats with induced sepsis

“…-Sham Group (GS): The animals were used as normal standard for biochemical and histological analysis; the animals underwent the same surgical techniques, but without the performance of CLP; -Sepsis Group (GSep): Animals were only realized the cecal ligation and puncture (CLP) [8]; -Veronica Group (GV): Administered Veronica officinalis extract (10ml/kg/day) once daily by gavage for seven days [9], without the performance of cecal ligation and puncture (CLP); -Sepsis-Veronica Group (GSep-V): Administered Veronica officinalis extract (10ml/kg/day) once daily by gavage from the first until the seventh day of the CLP. The animals were anesthetized with ketamine hydrochloride (100 mg/Kg) and xylazine hydrochloride (10 mg/Kg), intraperitoneally.…”