Diclofenac topical gel in excisional wounds maintain heal quality and reduce phlogistic signals1

Abstract

Brazil. Conception, design, intellectual and scientific content of the study, critical revision. ABSTRACT PURPOSE:To investigate diclofenac topical gel as an alternative to reduce phlogistic signals and maintain quality of wound repair. METHODS:Fifteen Wistar rats were used in this study; four excisional wounds were performed on the dorsum of each animal. Once in a day, cranial wounds received topical diclofenac gel administration and caudal wounds were washed with isotonic saline. After seven, 14 and 21 postoperative days, five animals were randomly chosen for macroscopic and microscopic wound analysis. RESULTS:On the 7 th day: diclofenac wounds showed significant higher scab formation, however showed less phlogistic signal; diclofenac wounds had larger area and had less neutrophil invasion. On the 14 th day: No area difference was noted and diclofenac wounds showed less hyperemia and phlogistic signals; diclofenac wounds showed greater keratinocytes invasion. On the 21 st day:Almost all wounds were closed and there were no difference regarding the type of scar formation; diclofenac wounds showed greater monocytes invasion and lower angiogenesis level. No difference was noted in any postoperative day regarding fibroblast invasion, collagen deposit quantity and quality. CONCLUSION:Diclofenac topical gel is capable of reducing phlogistic signals and do not cause fibroblast or keratinocyte downregulation thus do not lead to excisional wound healing impairment.

Ionic Hydrogel Based on Chitosan Cross-Linked with 6-Phosphogluconic Trisodium Salt as a Drug Delivery System

“…For this, the evolution of surgically created full thickness wounds at the dorsum of Wistar rats has been evaluated. The animal model used is one of the most common for healing studies. − The size of wounds created (3 cm in diameter) has been established based on previous studies which show that when the area is smaller, self-regeneration of the tissue dominates the process and the healing effect of the formulations tested is not appreciated. In any case, due to the self-regeneration, the most important information on these results is obtained the first days after causing the injury.…”

Nano-hybrid electrospun non-woven mats made of wool keratin and hydrotalcites as potential bio-active wound dressings

“…The topical administration of diclofenac has been demonstrated to be capable of reducing phlogistic signals without causing fibroblast or keratinocyte downregulation and thus without leading to excisional wound healing impairment. 19 PEO was used as a supporting polymer for electrospinning. However, despite the aforementioned advantages of keratinrich scaffolds, their weak mechanical strength and their fast biodegradation are recognized as the main critical problems that could limit their applications as scaffolds for skin tissue regeneration and controlled drug release.…”

Ação do diclofenaco de sódio e do celecoxibe na cicatrização por segunda intenção de feridas cutâneas de ratos wistar tratados com óleo de girassol

“…Em estudo abordando a evolução da cicatrização de queimaduras profundas em ratos Wistar, a autora observou a formação de crosta a partir do terceiro dia pós-indução, sendo mais espessa no grupo tratado com diclofenaco no sétimo dia de tratamento (DEBIASI, 2017). Costa et al (2014), observou a mesma característica ao estudar o efeito do diclofenaco gel tópico em feridas de ratos, onde a aplicação do gel causou uma redução dos sintomas flogísticos (sinais de inflamação) nas primeiras semanas de pós-operatório, sem causar a formação de tecido de cicatrização anormal, porém houve uma maior formação de crosta durante a fase precoce do processo cicatricial. Em nossos resultados macroscópicos observamos uma maior formação de crosta no grupo tratado com celecoxibe em relação ao controle e diclofenaco.…”