Brazil. Conception, design, intellectual and scientific content of the study, critical revision. ABSTRACT PURPOSE:To investigate diclofenac topical gel as an alternative to reduce phlogistic signals and maintain quality of wound repair. METHODS:Fifteen Wistar rats were used in this study; four excisional wounds were performed on the dorsum of each animal. Once in a day, cranial wounds received topical diclofenac gel administration and caudal wounds were washed with isotonic saline. After seven, 14 and 21 postoperative days, five animals were randomly chosen for macroscopic and microscopic wound analysis. RESULTS:On the 7 th day: diclofenac wounds showed significant higher scab formation, however showed less phlogistic signal; diclofenac wounds had larger area and had less neutrophil invasion. On the 14 th day: No area difference was noted and diclofenac wounds showed less hyperemia and phlogistic signals; diclofenac wounds showed greater keratinocytes invasion. On the 21 st day:Almost all wounds were closed and there were no difference regarding the type of scar formation; diclofenac wounds showed greater monocytes invasion and lower angiogenesis level. No difference was noted in any postoperative day regarding fibroblast invasion, collagen deposit quantity and quality. CONCLUSION:Diclofenac topical gel is capable of reducing phlogistic signals and do not cause fibroblast or keratinocyte downregulation thus do not lead to excisional wound healing impairment.
Background: The use of debriding drugs is not common in a situation of acute wound healing, despite its widespread use in chronic wound healing, with satisfactory results. Collagenase is one of the most used debriding drugs, and its application after a non-stitched skin wound biopsy could lead to a better wound healing outcome and less complication. Methods and Findings:Fifteen Wistar rats were used in this study; four excisional wounds were performed on the dorsum of each animal. Once in a day, collagenase cream was applied in the cranial wounds, while the caudal wounds were washed with isotonic saline. After 7, 14 and 21 postoperative days, 5 animals were randomly chosen for macroscopic and microscopic wound analysis.At the 7 th postoperative day: collagenase cream induced greater monocytes invasion, fibroblast invasion, collagen deposit, angiogenesis and reepithelialization. 14 th postoperative day: collagenase cream induced greater neutrophils invasion, fibroblasts invasion, collagen deposit, reepithelialization and higher proportion of type I collagen fibers. 21 st postoperative day: collagenase cream led to greater angiogenesis and reepithelialization. Conclusion:Collagenase cream led to a better wound healing outcome than simply saline rinse, induced earlier angiogenesis and inflammatory step; greater collagen deposit and reepithelialization.
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