Comparative study of tissue reactivity to n-butyl-2-cyanoacrylate and nylon monofilament thread on pericranium-cutaneous flaps in rats

Abstract

PURPOSE:To study the repair of pericranium-cutaneous flaps fixed with suture anchored in a skull bone tunnel or N-butyl-2-cyanoacrylate adhesive in Wistar rats with emphasis on the cellular inflammatory response and the production of types I and III collagen. METHODS:The operated region in the cephalic region of Wistar rats was removed minutes before euthanasia, fixed in formalin, and subjected to histological preparation. Slides were stained with hematoxylin-eosin and Picrosirius. Standardized counts of polymorphonuclear and mononuclear cells, fibroblasts, and macrophages were performed, and the percentages of types I and III collagen were determined. Data collection occurred on days 3, 7, 14, 21, and 45 postoperatively. A value of p<0.05 was considered statistically significant. RESULTS:Quantitative analysis of the data showed more fibroblasts in the surgical adhesive group than in the nylon monofilament thread groups (p=0.0211). Qualitative analysis showed higher reactivity in the adhesive group, with a predominance of polymorphonuclear cells from days 3−45 and macrophages from days 3−7. The amount of type I collagen exceeded 80% in the treated and control groups at the end of the experiment. CONCLUSIONS:Subperiosteal detachment triggers a cellular inflammatory response that is amplified using soft tissue fixation methods.The adhesive n-butyl-2-cyanoacrylate was more reactive than the nylon monofilament thread anchored in the skull bone tunnel.

Evaluation of Tissue Response in Sites Sutured with Cyanoacrylate and Submitted to Low Power Laser Therapy: A Randomized Clinical Trial

“…Some studies have demonstrated the efficacy of using sutures with adhesive compounds in surgery. These have the advantage of not reducing tissue perfusion caused by conventional suture and demonstrate inflammatory reactions similar to conventional or even minor suture materials in the final stages of inflammation [4,16,17]. In a comparative study between cyanoacrylate and nylon suture, cyanoacrylate has been shown to promote greater recruitment of polymorphonuclear leukocytes [18].…”