Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect

Guedes, Francisco Sobreira; Cruz, Deyvid Santos da; Rodrigues, Marcela Marcondes Pinto; Silva, Leopoldo Muniz da; Amorim, Renée Laufer; Vianna, Pedro Thadeu Galvão; Castiglia, Yara Marcondes Machado

doi:10.1590/s0102-86502012000100007

Cited by 6 publications

(5 citation statements)

References 13 publications

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“…The hemorrhage group pretreated with placebo presented a higher percentage of tubular dilation and degeneration if compared to the group that received parecoxib, indicating that previous use of parecoxib prevented tubular injuries in this situation. Likewise, Guedes et al [ 39 ] observed a lower degree of acute tubular necrosis in rats subjected to acute hemorrhage and pretreated with nonselective NSAID (ketoprofen). Our findings corroborate the protective effects of parecoxib against renal tubular injury of hypoperfusion.…”

Section: Discussionmentioning

confidence: 98%

Effects of a Single Dose of Parecoxib on Inflammatory Response and Ischemic Tubular Injury Caused by Hemorrhagic Shock in Rats

Takaku

Silva

Iritsu

et al. 2018

Pain Research and Treatment

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Parecoxib, a selective COX-2 inhibitor, is used to improve analgesia in postoperative procedures. Here we evaluated whether pretreatment with a single dose of parecoxib affects the function, cell injury, and inflammatory response of the kidney of rats subjected to acute hemorrhage. Inflammatory response was determined according to serum and renal tissue cytokine levels (IL-1α, IL-1β, IL-6, IL-10, and TNF-α). Forty-four adult Wistar rats anesthetized with sevoflurane were randomized into four groups: placebo/no hemorrhage (Plc/NH); parecoxib/no hemorrhage (Pcx/NH); placebo/hemorrhage (Plc/H); and parecoxib/hemorrhage (Pcx/H). Pcx groups received a single dose of intravenous parecoxib while Plc groups received a single dose of placebo (isotonic saline). Animals in hemorrhage groups underwent bleeding of 30% of blood volume. Renal function and renal histology were then evaluated. Plc/H showed the highest serum levels of cytokines, suggesting that pretreatment with parecoxib reduced the inflammatory response in rats subjected to hemorrhage. No difference in tissue cytokine levels between groups was observed. Plc/H showed higher percentage of tubular dilation and degeneration, indicating that parecoxib inhibited tubular injury resulting from renal hypoperfusion. Our findings indicate that pretreatment with a single dose of parecoxib reduced the inflammatory response and tubular renal injury without altering renal function in rats undergoing acute hemorrhage.

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Section: Discussionmentioning

confidence: 98%

Effects of a Single Dose of Parecoxib on Inflammatory Response and Ischemic Tubular Injury Caused by Hemorrhagic Shock in Rats

Takaku

Silva

Iritsu

et al. 2018

Pain Research and Treatment

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“…It should be noted that it is a fact that the kidney in extremely preterm infants (26–30 gestation weeks) retains the ability to nephrogenesis after birth , but this period is limited to 40 days for newborns with extremely low birth weight (≤ 1000 g) . Unfortunately, the functionality of these nephrons is limited: experiments on primates demonstrated high levels of injuries among the glomeruli formed postnatally . Most researchers believe that rats of age 7 days correspond to a term or near‐term infants and with a certain degree of approximation we can extrapolate it to the kidney considering the fact that despite morphological and functional maturity, by some parameters (e.g., by proliferation) the renal tissue in this period retains features of preterm kidney.…”

Section: Discussionmentioning

confidence: 99%

The role of oxidative stress in acute renal injury of newborn rats exposed to hypoxia and endotoxin

et al. 2017

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Neonatal kidney injury is a frequent pathology, especially among premature infants. The search for effective nephroprotection requires the creation of adequate experimental models of nephropathy in newborns. In this study, we explored the development of acute kidney injury (AKI) in neonatal rats during hypoxia or administration of endotoxin. We found that 2-h hypoxia (8% O ) and the intraperitoneal injection of 4 mg·kg lipopolysaccharide (LPS) causes the appearance of AKI markers, such as kidney injury molecule-1 (КIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) in the rat urine after 24 and 72 h of exposure. On the other hand, the levels of blood urine nitrogen under the same conditions rise only slightly. The damaging effects of hypoxia and endotoxin were accompanied by histological changes in the renal tissue and a significant decrease in the proliferation marker, (proliferating cell nuclear antigen). It is revealed that 3 h after the introduction of LPS, levels of reactive oxygen species in the kidney were significantly increased, and the injection of the antioxidant N-acetylcysteine afforded protection from AKI, evaluated by urine КIM-1 and NGAL levels. Thus, the simulation of AKI in newborn rat pups can be employed in screening for potential nephroprotective drugs, particularly among antioxidative compounds to be used in neonatology.

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“…Todas as lâminas foram analisadas em microscópio óptico. As alterações histopatológicas dos rins e da bexiga foram classificadas em ausentes, leves, moderadas e acentuadas, de acordo com Guedes Jr et al (2012).…”

Section: Methodsunclassified

Avaliação clínica, laboratorial e anatomopatológica do sistema urinário de ovinos confinados com ou sem suplementação de cloreto de amônio

et al. 2016

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Resumo: A acidificação urinária com cloreto de amônio (CA) é um método preventivo eficiente em urolitíase obstrutiva em ovinos. Os objetivos deste estudo com ovinos confinados, que receberam dieta concentrada com elevado teor proteico, foram: verificar o efeito da dieta sobre a formação de urólitos e o desenvolvimento da doença; analisar as características macroscópicas e histopatológicas do sistema urinário; relacionar os achados clínicos, laboratoriais e necroscópicos com a presença de urólitos. Utilizaram-se 60 ovinos machos: grupo CA (n=40), 400 mg/kg CA/dia, tratados via oral, por 42 dias consecutivos; grupo-controle (n=20), não tratado. Determinaram-se sete momentos de colheita de amostras com intervalos de sete dias, no total de 56 dias de confinamento. Encontraram-se microcálculos na pelve renal em cinco animais de ambos os grupos. As lesões renais microscópicas mais relevantes foram congestão vascular e necrose tubular. Concluiu-se que a dieta rica em concentrado provocou lesão renal em ambos os grupos, embora sem alterar a função renal, o que foi comprovado em testes pela ureia e creatinina séricas. O cloreto de amônio fornecido ao grupo CA não impediu a calculogênese, mas reduziu sua prevalência em relação ao grupo-controle. Os ovinos do grupo-controle tiveram maior comprometimento renal, pela alta incidência de cristalúria e pela necrose tubular, induzidas pelo consumo da dieta rica em grãos.

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Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect

Abstract: Ketoprofen was more protective to the rat kidney in condition of anesthesia with sevoflurane and hypovolemia, but it seems that TNF-α and IL-1 were not involved in that protection.

Cited by 6 publications

References 13 publications

Effects of a Single Dose of Parecoxib on Inflammatory Response and Ischemic Tubular Injury Caused by Hemorrhagic Shock in Rats

Effects of a Single Dose of Parecoxib on Inflammatory Response and Ischemic Tubular Injury Caused by Hemorrhagic Shock in Rats

The role of oxidative stress in acute renal injury of newborn rats exposed to hypoxia and endotoxin

Avaliação clínica, laboratorial e anatomopatológica do sistema urinário de ovinos confinados com ou sem suplementação de cloreto de amônio

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