Effects of a Single Dose of Parecoxib on Inflammatory Response and Ischemic Tubular Injury Caused by Hemorrhagic Shock in Rats

Abstract

Parecoxib, a selective COX-2 inhibitor, is used to improve analgesia in postoperative procedures. Here we evaluated whether pretreatment with a single dose of parecoxib affects the function, cell injury, and inflammatory response of the kidney of rats subjected to acute hemorrhage. Inflammatory response was determined according to serum and renal tissue cytokine levels (IL-1α, IL-1β, IL-6, IL-10, and TNF-α). Forty-four adult Wistar rats anesthetized with sevoflurane were randomized into four groups: placebo/no hemorrhage (Plc/NH); parecoxib/no hemorrhage (Pcx/NH); placebo/hemorrhage (Plc/H); and parecoxib/hemorrhage (Pcx/H). Pcx groups received a single dose of intravenous parecoxib while Plc groups received a single dose of placebo (isotonic saline). Animals in hemorrhage groups underwent bleeding of 30% of blood volume. Renal function and renal histology were then evaluated. Plc/H showed the highest serum levels of cytokines, suggesting that pretreatment with parecoxib reduced the inflammatory response in rats subjected to hemorrhage. No difference in tissue cytokine levels between groups was observed. Plc/H showed higher percentage of tubular dilation and degeneration, indicating that parecoxib inhibited tubular injury resulting from renal hypoperfusion. Our findings indicate that pretreatment with a single dose of parecoxib reduced the inflammatory response and tubular renal injury without altering renal function in rats undergoing acute hemorrhage.

Opioid-Free Anesthesia for Laparoscopic Hysterectomy: Is it Appropriate?

“…The reported beneficial effects of OFA could be attributed to varied effects of each of the used drugs; namely parecoxib sodium, dexmedetomidine and lidocaine, to induce and maintain OFA. Such attribution was in line with Guo et al [27] who suggested that COX-2 inhibitor parecoxib exerts its analgesic effect on surgical pain through the inhibition of spinal cord neuronal extracellular signal-regulated kinase activation and Takaku et al [28] experimentally found pretreatment with a single dose of parecoxib reduced the inflammatory response with attenuation of serum and tissue levels of IL-1α, IL-1β, IL-6, IL-10, & TNF-α.…”

Correlation between perioperative parecoxib use and postoperative acute kidney injury in patients undergoing non-cardiac surgery: a retrospective cohort analysis

“…For example, the study of Takaku et al also showed that a single-dose of parecoxib (20 mg/kg) reduced tubular renal injury and serum inflammatory cytokines level (interleukin (IL)-1α, IL-β, IL6, and tumour necrosis factor-α) in an ischaemic rat model. 22 Moreover, several animal studies(Takaku et al, Feitoza et al, Feitoza et al, Candelario-Jalil et al) have suggested that pretreatment with COX-2 inhibitors improved outcomes in function and histology in not only the kidney but also other organs after ischaemia. [22][23][24][25] To the best of our knowledge, this is the first clinical report to suggest that single-dose (40 mg or 80 mg) parecoxib may be renoprotective in patients aged 18-60 years who underwent non-cardiac surgery.…”

“…22 Moreover, several animal studies(Takaku et al, Feitoza et al, Feitoza et al, Candelario-Jalil et al) have suggested that pretreatment with COX-2 inhibitors improved outcomes in function and histology in not only the kidney but also other organs after ischaemia. [22][23][24][25] To the best of our knowledge, this is the first clinical report to suggest that single-dose (40 mg or 80 mg) parecoxib may be renoprotective in patients aged 18-60 years who underwent non-cardiac surgery.…”

Correlation between perioperative parecoxib and postoperative acute kidney injury in patients undergoing non-cardiac surgery: a retrospective cohort analysis

“…For example, a study also showed that a single-dose of parecoxib (20 mg/kg) reduced tubular renal injury and serum in ammatory cytokines level (interleukin (IL)-1α, IL-β, IL6, and tumor necrosis factor (TNF)-α) in an ischemic rat model [21]. Moreover, a number of animal studies have suggested that pretreatment with COX-2 inhibitors improved outcomes in function and histology not only in the kidney but also in other organs after ischemia [21,22,24]. To the best of our knowledge, this is the rst clinical report to suggest that parecoxib may be renoprotective in patients aged 18-60 years who underwent non-cardiac surgery.…”