Effects of abdominal sepsis in the healing of abdominal wall: experimental study in rats

Brandão, Alexandre Malta; Silva, Naiara Galvão da; Oliveira, Marcos Vinícius Melo de; Morais, Pedro Henrique Alves de; Silva, Silvana Marques e; Sousa, João Batista de; Carneiro, Fabiana Pirani

doi:10.1590/s0102-86502011000800008

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…The losses may have been associated with surgical stress 14 . Weight loss has been described in previous studies on wound healing 15,16 . Others reported weight recovery as early as the seventh POD 17 .…”

Section: Discussionmentioning

confidence: 88%

Evaluation of the effects of S-methylisothiourea hemisulfate, an inducible nitric oxide synthase inhibitor, on the healing of colonic anastomosis in rats

et al. 2012

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PURPOSE:To evaluate the effects of S-methylisothiourea hemisulfate (SMT) on the healing of colonic anastomosis in rats. METHODS:Sixty rats Wistar were distributed into two groups of 30 animals: experimental (E) and control C). The animals of experimental group received intraperitoneal SMT at 50mg/kg/dose every 12 hours for 72 hours. The control group received intraperitoneal saline at the same volume of SMT. The rats were subdivided into subgroups groups of 10 for euthanasia on the third, seventh, and 14 th postoperative days (POD). We evaluated clinical and weight evolution, breaking strength and histopathology; also, a blood sample was collected for serum dosage of nitrite/nitrate. RESULTS:There was more vascular neoformation (p=0.006) and granulation (p=0.002) in the E3 group, and more mononuclear infiltrates in the C3 group (p=0.041). There was also more edema in the C14 group (p=0.008). There was no statistically significant difference in breaking strength, nitrite/nitrate dosage, and the remaining histopathological parameters. CONCLUSION:The use of S-methylisothiourea hemisulfate improved the healing of colonic anastomosis in rats on the third postoperative day by accelerating the proliferative stage of healing, but without interfering with the breaking strength of the anastomosis. Key words: Nitric Oxide. Nitric Oxide Synthase. Wound Healing. Anastomosis, Surgical. Colon. Rats. RESUMO OBJETIVO:Avaliar os efeitos do hemissulfato de S-metilisotiouréia (SMT) na cicatrização de anastomoses colônicas em ratos no terceiro, sétimo e 14° dia de pós-operatório (DPO). MÉTODOS: Sessenta ratos Wistar foram distribuídos em dois grupos: experimental (E) e controle (C), com 30 animais cada. No grupoexperimental foi administrado SMT 50mg/kg/dose, intraperitoneal a cada 12 horas por 72 horas. O grupo controle recebeu NaCl a 0,9%.Os ratos foram subdivididos em grupos de 10 para eutanásia no terceiro, sétimo e 14° DPO. Avaliou-se a evolução clínica e o peso dos animais, a resistência tênsil e histopatologia da anastomose, e a dosagem de nitrito/nitrato no soro. RESULTADOS:Houve mais neoformação vascular (p=0,006) e de granulação (p=0,002) no grupo E3, e maior infiltração de mononucleares no grupo C3 (p=0,041). Houve também mais edema no grupo C14 (p=0,008). Não houve diferença estatisticamente significativa na resistência tênsil, a dosagem de nitrito / nitrato, e os restantes parâmetros histopatológicos. CONCLUSÃO:A utilização do hemissulfato de S-metilisotiouréia acelerou a cicatrização das anastomoses colônicas, a melhoria ocorreu no terceiro DPO: verificou-se que a fase proliferativa da cicatrização foi acelerada. Não houve interferência na resistência tênsil das anastomoses.Descritores: Óxido Nítrico. Oxido Nítrico Sintase. Cicatrização. Anastomose Cirúrgica. Colo. Ratos. Evaluation of the effects of S-methylisothiourea hemisulfate, an inducible nitric oxide synthase inhibitor, on the healing of colonic anastomosis in rats

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Section: Discussionmentioning

confidence: 88%

Evaluation of the effects of S-methylisothiourea hemisulfate, an inducible nitric oxide synthase inhibitor, on the healing of colonic anastomosis in rats

et al. 2012

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“…Nevertheless, these patients frequently require surgical procedures, and the inadequate healing of their wounds can cause a number of complications, such as the dehiscence of sutures of the abdominal wall, which is an incident associated with high rates of morbidity and death. A decrease of tensile strength in intestinal anastomoses and sutures of the abdominal wall, has been shown in studies with rats with peritoneal sepsis [8][9][10] . Thus, the detection of factors relating to deficient healing in patients with sepsis will be able to improve therapeutic procedures 6 .…”

Section: Introductionmentioning

confidence: 85%

“…Concerning to the patients with sepsis, generally admitted to intensive care units, these are subjected not only to a higher risk of death, but are also vulnerable to developing problems related to deficient healing. This is a result of the interruption of repairing and remodelling events, once, during sepsis, there ishighoxidative stress, insufficient collagen production, and exaggerated immune response, with high levels of proinflammatory cytokines, especially TNF- [5][6][7][8] .…”

Section: Introductionmentioning

confidence: 99%

The effect of abdominal sepsis in the healing of skin on diabetic rats

Lima

Braz²,

Carvalho³

et al. 2015

J Surg Clin Res

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Background/purpose: There is an increasing discussion concerning the deleterious effect of hyperglycemia on the wound healing. Patients with sepsis are subjected not only to high risk of death, but are also vulnerable to developing problems related to deficient healing. The aim of this study was to examine the interference of abdominal sepsis and diabetes on the healing of skin in a rat experimental model. Methods: Wistar rats (Rattus norvegicus) weighing 282±34g randomly distributed into 4 groups of 6 animals each. Twelve animals were subjected to abdominal sepsis with cecal ligation and puncture (CLP) and 12 without sepsis. Six animals from each subgroup had induction of diabetes induced with streptozotocin 50 mg/kg, i.p. and six were non-diabetic. Six days after sepsis induction, a tensile strength test of skin scar from abdominal wall and histopatology of skin wound were carried out. Results: The tensile strength showed a significant difference between groups. The control group rats had a tensile strength of 219.0±12.4 gf/cm2, significantly higher than in the diabetes group (185.2±5,9gf/cm2) and in the sepsis group (107.0±9,8mg/cm2) respectively. The group of animals subjected to sepsis + diabetes had the lowest mean tensile strength (86.3±6,6gf/cm2), significantly lower than the results from other groups (p<0.0001). The isolated sepsis was more detrimental to wound healing than the isolated diabetes. The existence of the two comorbidities resulted in the lower tensile strength of the scar tissue. The control group rats exhibited an inflammatory reaction score (4.67±0.16) significantly lower (p=0.002) than the scores exhibited by the sepsis + diabetes group (5.98 ± 0.27), the sepsis group (5.04 ± 0.23) and the diabetes group (4.95 ± 0.32). Conclusion: Sepsis and diabetes, alone or in combination, negatively influenced the healing of skin wounds in rats. Therefore, the data indicates that sepsis and diabetes may operate as deleterious to the wound healing.

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Evaluation of peritoneal reinfection after fecal peritonitis

Alberti¹,

Petroianu²

2012

Acta Cir. Bras.

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PURPOSE:To investigate the role of a previous abdominal infection on peritonitis. METHODS:Twenty-seven adult female Wistar rats were submitted to fecal peritonitis by an intraperitoneal injection of a solution of rat feces. The animals were divided into three groups (n = 9 each): Group 1 -control -intraperitoneal injection of an amount of fecal solution known to be lethal (10 ml/kg), Group 2 -reinfection -intraperitoneal injection of an amount of fecal solution known not to be lethal (2 ml/kg) followed by an injection of fecal solution (10 ml/kg) 30 days later, Group 3 -late reinfection -intraperitoneal injection of 2 ml/kg feces followed by an injection of 10 ml/kg four months later. RESULTS:All animals in Group 1 died within seven days after injection of the fecal solution. In contrast, in the pre-infected Group 2 only one animal died 24 hours after injection of the fecal solution (10 ml/kg). In Group 3, eight of the nine animals in each subgroup died over a period of seven days. The difference in survival time between groups 1, 2 and 3 was for p = 0.0042 (logrank test). CONCLUSIONS:Milder peritoneal sepsis due to fecal infection raises the organic resistance to a new more intense fecal contamination occuring after a short period of time. However, this protection did not persist over a prolonged period of time.Key words: Peritonitis. Sepsis. Infection. Feces. Immunity. Rats. RESUMO OBJETIVO:Investigar o efeito de uma nova infecção peritoneal após realização de peritonite fecal prévia. MÉTODOS:Foram utilizados 27 ratos fêmea Wistar adultos submetidos à peritonite fecal com injeção intraperitoneal de uma solução de fezes de ratos. Os animais foram distribuidos em três grupos (n = 9): Grupo 1 -controle: injeção intraperitoneal de solução de fezes com uma quantidade sabidamente letal (10 ml/kg); Grupo 2 -reinfecção: injeção intraperitoneal de solução de fezes com uma quantidade sabidamente não letal (2 ml/kg) e, após 30 dias, injeção de solução de fezes (10 ml/kg); Grupo 3 -reinfecção tardia: injeção intraperitoneal de fezes a 2ml/kg e, após quatro meses, injeção de 10ml/kg. RESULTADOS:Todos os animais do Grupo 1 morreram dentro de sete dias após a injeção da solução de fezes. Em contraste, no grupo de reinfecção precoce Grupo 2 apenas um animal morreu 24 horas após a injecção da solução de fezes (10 ml / kg). No Grupo 3, oito dos nove animais morreram durante um período de sete dias. A diferença no tempo de sobrevivência entre os grupos 1, 2 e 3 foi de p = 0,0042 (teste de logrank). CONCLUSÕES:Uma sepse peritoneal menor por fezes eleva a resistência orgânica a nova contaminação fecal mais intensa que ocorra após um período curto. Contudo, essa defesa não persiste por tempo mais prolongado.Descritores: Peritonite. Sepse. Infecção. Fezes. Imunidade. Ratos. Evaluation of peritoneal reinfection after fecal peritonitis

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Effects of abdominal sepsis in the healing of abdominal wall: experimental study in rats

Cited by 4 publications

References 15 publications

Evaluation of the effects of S-methylisothiourea hemisulfate, an inducible nitric oxide synthase inhibitor, on the healing of colonic anastomosis in rats

Evaluation of the effects of S-methylisothiourea hemisulfate, an inducible nitric oxide synthase inhibitor, on the healing of colonic anastomosis in rats

The effect of abdominal sepsis in the healing of skin on diabetic rats

Evaluation of peritoneal reinfection after fecal peritonitis

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