Electro-acupuncture reduces the need for additional anesthetics in experimental studies

Silva, Agamenon Honório; Neto, Alberico Ximenes do Prado; Guimarães, Sérgio Botelho

doi:10.1590/s0102-86502010000400015

Cited by 3 publications

(3 citation statements)

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“…Many papers have demonstrated the effects of MA and EA on ST-36 acupoint in attenuating oxidative stress in different experimental diseases [5][6][7][8][9] . Furthermore, the use of ketamine+xylazine, an anesthetic mixture often used in experimental studies may induce some degree of oxidative stress in a health animal [12][13] . It has been previously shown that large amounts of endogenous opioid peptides are secreted from the adrenal glands when CV-12 acupoints are stimulated using acupuncture with 2Hz 14 .…”

Section: Introductionmentioning

confidence: 99%

Electroacupuncture attenuates liver and kidney oxidative stress in anesthetized rats

Silva¹,

Figueiredo²,

Dias³

et al. 2011

Acta Cir. Bras.

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PURPOSE:Investigate the effects of a single electroacupuncture (EA) session at acupoints Zusanli (ST-36) and Zhongwan (CV-12) combined in regulating oxidative stress in liver and kidney in anesthetized rats. METHODS: Eighteen healthy rats randomly assigned to 3 groups (n=6) were anesthetized intraperitoneally with ketamine (90mg kg -1 body weight) + xylazine (10mg/kg body weight): G-1: Control (anesthesia), G-2: anesthesia+EA10Hz and 10 mA, 10 Hz) applied to right ST-36 and CV-12 acupoints for 30 minutes. G-3 was likewise treated, using a tenfold higher frequency (100 Hz). G6PDH activity, malondialdehyde (MDA) and glutathione (GSH) levels were assayed spectrophotometrically. RESULTS: Liver MDA and GSH concentrations increased significantly in rats submitted to EA 10Hz (p<0.01) and EA 100Hz (p<0.001), compared with control G-1. Liver and kidney G6GPH activity decreased significantly in G-2 (p<0.01) and G-3 (p<0.001) compared with G-1 in EA100Hz rats. A similar pattern was found in kidney G6PDH activity in EA10Hz rats. CONCLUSION: Single 30-minute EA 10/100Hz session enhances lipid peroxidation and simultaneously reduces oxidative stress in liver and kidney tissues in a rat model. Keywords: Acupuncture. Electroacupuncture. Lipid Peroxidation. Oxidative Stress. Rats. RESUMO OBJETIVO: Investigar os efeitos de uma única sessão de eletroacupuntura (EA) aplicada nos acupontos Zusanli (E-36) e Zhongwan (RM-12) simultaneamente, na regulação do estresse oxidativo no fígado e rins em ratos anestesiados. MÉTODOS: Dezoito ratos sadios, distribuídos aleatoriamente em três grupos (n = 6), foram anestesiados com cetamina (90mg/kg de peso) + xilazina (10mg/kg de peso): G-1: Controle (anestesia), G-2: anestesia + EA10Hz e G-3: anestesia + EA100Hz. Os ratos do grupo G-2 foram submetidos à EA (ondas quadradas pulsadas, 10 mA, 10 Hz) aplicada aos acupontos ST-36 direito e VC-12 por 30 minutos. Nos ratos do grupo G-3 utilizou-se uma freqüência dez vezes maior (100 Hz). A atividade da enzima G6PDH e as concentrações de malondialdeído (MDA) e glutationa (GSH) foram verificadas por espectrofotometria. RESULTADOS: As concentrações hepáticas de MDA e GSH aumentaram significativamente nos ratos submetidos à EA, utilizando 10Hz (p <0,01) e 100Hz (p <0,001), comparado com o controle. A atividade de G6GPH diminuiu significativamente no G-2 (p <0,01) e G-3 (p <0,001) no fígado e no rim em comparação ao grupo G-1 em ratos tratados com 100Hz. CONCLUSÃO: Uma única sessão de EA 10/100Hz por 30 minutos aumenta a peroxidação lipídica e simultaneamente reduz o estresse oxidativo no fígado e no rim de ratos sadios. Descritores: Acupuntura. Eletroacupuntura. Peroxidação de Lipídeos. Estresse Oxidativo. Ratos. Electroacupuncture attenuates liver and kidney oxidative stress in anesthetized rats

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Section: Introductionmentioning

confidence: 99%

Electroacupuncture attenuates liver and kidney oxidative stress in anesthetized rats

Silva¹,

Figueiredo²,

Dias³

et al. 2011

Acta Cir. Bras.

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“…21 Other anaesthetics Both acupuncture and EA prolong the anaesthetic effect of the ketamine-xylazine combination in rats, allowing a longer duration of anaesthesia with a lower dose of anaesthetic. 22 Rosted and colleagues 23,24 observed that the onset of a local anaesthetic was quicker if segmentally administered acupuncture was given before a regional inferior dental block. The faster onset of local anaesthetics may be as a result of increased local blood flow after acupuncture.…”

Section: Hypnoticsmentioning

confidence: 99%

Perioperative acupuncture modulation: more than anaesthesia

Lü

Dong

Wang

et al. 2015

British Journal of Anaesthesia

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Accumulated evidences from clinical trials and updated reviews suggest that the role of acupuncture in perioperative medicine extends beyond the classical scope of anaesthesia and has been underestimated. Perioperative acupuncture reduces not only the consumption of anaesthetics and analgesics, but also anaesthesia-related complications, and protects organs in the perioperative period. These beneficial effects make acupuncture a promising approach in perioperative management, especially with respect to enhanced surgery recovery and specific surgical populations, such as elderly patients and 'triple-low' patients. Furthermore, efforts have been made to optimize the clinical application of perioperative acupuncture.

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“…After different time intervals blood samples were collected in order to evaluate the levels of ibuprofen. Blood was collected by punctioning the abdominal aorta under anesthesia with xylazine (10 mg/kg) and ketamine (90 mg/kg) given intraperitoneally (Silva et al, 2010). The samples were transferred to heparinized tubes and centrifuged at 3000 rpm for ten minutes (Nova Técnica centrifuge, model NT812).…”

Section: Experimental Protocolmentioning

confidence: 99%

Microspheres prepared with biodegradable PHBV and PLA polymers as prolonged-release system for ibuprofen: in vitro drug release and in vivo evaluation

Bazzo

Macedo

Crenca

et al. 2012

Braz. J. Pharm. Sci.

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In this study, poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and poly(l-lactide) (PLA) microspheres containing ibuprofen were prepared with the aim of prolonging the drug release. The oil-in-water (O/W) emulsion solvent evaporation technique was used, varying the polymer ratio. All formulations provided spherical particles with drug crystals on the surface and a porous and rough polymeric matrix when PHBV was used and smooth external surface when prepared with PLA. The in vitro dissolution profiles show that the formulation containing PHBV/PLA at the proportion of 30/70 presented the best results in terms of prolonging the ibuprofen release. The analysis of the concentration of ibuprofen in the blood of rats showed that maximum levels were achieved at between one and two hours after administration of the immediate-release form (pure drug), while the prolonged microspheres led to a small amount of the drug being released within the first two hours and reached the maximum level after six hours of administration. It was concluded that it is possible to prolong the release of ibuprofen through its incorporation into PHBV/PLA microspheres.Uniterms: Microspheres/preparation. Poly(hydroxybutyrate-co-hydroxyvalerate). Poly(l-lactide). Ibuprofen/release. Drugs/controlled release/in vitro study. Drugs/controlled release/in vivo evaluation.No presente estudo foram preparadas microesferas de poli(hidroxibutirato-co-hidroxivalerato) (PHBV) e poli(ácido láctico) (PLA) com o objetivo de prolongar a liberação do ibuprofeno, utilizado como fármaco modelo. Empregou-se o método de emulsificação e evaporação do solvente óleo em água (O/A), variando-se a proporção entre os polímeros. Todas as formulações originaram partículas esféricas com cristais de fármaco aderidos à superfície externa. As microesferas apresentaram superfície rugosa e porosa, quando o PHBV foi utilizado, e superfície externa lisa, quando preparadas com o PLA. Os perfis de dissolução in vitro evidenciaram que a formulação que continha PHBV/PLA na proporção de 30/70 apresentou melhores resultados para prolongar a liberação do ibuprofeno. Através da análise da concentração de ibuprofeno no plasma de ratos, após administração oral, verificou-se que os níveis máximos ocorreram entre 1 e 2 horas após a administração de ibuprofeno não encapsulado, enquanto o fármaco presente nas microesferas atingiu um pico máximo após 6 horas da administração. Conclui-se, portanto, que é possível prolongar a liberação do ibuprofeno após a sua incorporação às microesferas preparadas com os polímeros PHBV e PLA, especialmente na proporção de 30/70. Unitermos: Microesferas/preparação. Poli(hidroxibutirato-co-hidroxivalerato). Poli(ácido láctico). Ibuprofeno/liberação. Fármacos/liberação controlada/estudo in vitro. Fármacos/liberação controlada/ avaliação in vivo.

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Electro-acupuncture reduces the need for additional anesthetics in experimental studies

Abstract: Both the AC and the EAC10/100 prolong the anesthetic effect of the combination Ketamine-xylazine in rats, allowing longer duration of anesthesia with a lower dose of anesthetic, thereby reducing the occurrence of complications.

Cited by 3 publications

References 15 publications

Electroacupuncture attenuates liver and kidney oxidative stress in anesthetized rats

Electroacupuncture attenuates liver and kidney oxidative stress in anesthetized rats

Perioperative acupuncture modulation: more than anaesthesia

Microspheres prepared with biodegradable PHBV and PLA polymers as prolonged-release system for ibuprofen: in vitro drug release and in vivo evaluation

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