Angiogenesis, fibrinogenesis and presence of synechiae after exeresis of a swine vocal fold mucosal microflap and use of topical mitomycin-C

Abstract

PURPOSE: To evaluate swine vocal fold healing in a period of 30 days after topical mitomycin-C application. METHODS: Twelve swine underwent exeresis of mucosal flaps from the free edge of the anterior third of both vocal folds with a cold instrument (laryngeal scissors). The animals were divided into two groups: EG (Experimental Group), consisting of 6 animals undergoing topical MMC application (4 mg/dL) on the operated area for 4 minutes; CG (Control Group), 6 animals undergoing topical saline solution application on the operated area for 4 minutes. After 30 days, the animals were sacrificed and the larynges were collected and examined for the presence of synechiae as well as a histological immunohistochemical assessment of immature and mature collagen deposition, number of blood vessels and myofibroblasts. RESULTS: Mature collagen deposition in the EG was 452.12 μm² and 1332.31μm² in the CG; immature collagen deposition was 1511.73μm² in the EG and 1020.61μm² in the CG. The number of myofibroblasts was 1.556 in the EG and 3.583 in the CG. The number of blood vessels was 2.565 in EG and 6.917 in the CG. There were no synechiae in the two studied groups. CONCLUSIONS: There was an increase in immature collagen deposition in the experimental group when compared with the control group. There was a decrease in mature collagen deposition in the experimental group when compared with the control group. There was a decrease in the number of myofibroblasts in the experimental group when compared with the control group. A decrease in blood vessels was observed in the experimental group when compared with the control group. There was no synechia formation in either studied group.

Investigation of Experimental Wound Closure Techniques in Voice Microsurgery

“…Three of the more commonly used models in operative studies are rabbits (Thibeault, Gray et al 2002;Thibeault, Bless et al 2003;Branski, Rosen et al 2005;Carneiro and Scapini 2009;Campagnolo, Tsuji et al 2010), dogs (Garrett, Coleman et al 2000;Fleming, McGuff et al 2001;Rousseau, Hirano et al 2003;Karajanagi, Lopez-Guerra et al 2011) and pigs (Blakeslee, Banks et al 1995;Garrett, Coleman et al 2000;Jiang, Raviv et al 2001;Alipour and Jaiswal 2008;Fonseca, Malafaia et al 2010). …”

“…Extensive work has focused on improving wound closure methods to minimize scar tissue formation, ranging from micro-suturing which allows for primary healing (Woo 1995;Tsuji, Nita et al 2009), to the use of tissue adhesives like fibrin glue (Bleach, Milford et al 1997;Flock 2005;Kitahara, Masuda et al 2005;Finck, Harmegnies et al 2010;Skodacek, Arnold et al 2011), and the use of chemical agents (Campagnolo, Tsuji et al 2010) like Mitomycin-C (Branski, Verdolini et al 2006;Fonseca, Malafaia et al 2010) or stem cells (Hong, Lee et al) to enhance the healing process of vocal fold wounds. However, various challenges faced in the execution of voice microsurgery add to the complexity of wound closure.…”

Experimental study on the effects of bismuth subgallate on the inflammatory process and angiogenesis of the oral mucosa

“…The formation of new vessels is essential for tissue regeneration and, therefore, local factors, chemical mediators, extracellular matrix, and metabolic gradients show concomitant development. 7 …”