Systemic evaluation on ischemia and reperfusion injury of splanchnic organs in rats

Cerqueira, Nereide Freire; Hussni, Carlos Alberto; Yoshida, Winston Bonetti; Padovani, Carlos Roberto

doi:10.1590/s0102-86502009000400008

Cited by 9 publications

(8 citation statements)

References 18 publications

(23 reference statements)

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Section: Discussionsupporting

confidence: 57%

“…This shock can be consistently reproduced in a rat model using the same ischemic and reperfusion times of our previous similar experimental settings 3,10 . Many local and systemic modifications are involved in the development of this syndrome, the release of oxygen-derived free-radicals being very important and extensively studied 3 . During reperfusion there is a heavy production of free-radicals and the efficacy of the endogenous scavengers is not only compromised, but also decreased quantitatively inside the cell 17 .…”

Section: Discussionsupporting

confidence: 57%

“…The main clinical adverse consequence is a severe circulatory collapse known as splanchnic artery occlusion shock -SAOS 2,3 . Increased capillary permeability 4 , accumulation of oxygen-derived free radicals 5 , and alteration of the intestinal barrier 6 are the main contributors to the development of this syndrome.…”

Section: Introductionmentioning

confidence: 99%

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Effect of protective solutions and hydroxyethyl starch in the attenuation of the injuries of ischemia and reperfusion of splanchnic organs

et al. 2015

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PURPOSE: Vogt´s antioxidant solution (red blood cells, Ringer's solution, sodium bicarbonate, mannitol, allopurinol and 50% glucose) or its modification including hydroxyethyl starch (HES) were tested for the prevention of splanchnic artery occlusion shock. METHODS:Seventy rats were distributed in treatment (3), control (1), and sham (3) groups. Ischemia and reperfusion were induced by celiac, superior mesenteric and inferior mesenteric arteries occlusion for 40 min, followed by 60 min reperfusion or sham procedures.Controls received saline, both treatment and sham groups received the Vogt's solution, modified Vogt's solution (replacing Ringer's solution by HES), or HES. Mean arterial blood pressure (MABP), ileal malondialdehyde (MDA) and plasmatic MDA were determined, and a histologic grading system was used. RESULTS:At reperfusion, MABP dropped in all I/R groups. Only HES treatment was able to restore final MABP to the levels of sham groups. Plasmatic MDA did not show differences between groups. Ileum MDA was significantly higher in the control and treatment groups as compared to the sham group. Histology ranking was higher in the only in control group. CONCLUSIONS:Hydroxyethyl starch was able to prevent hemodynamic shock but not intestinal lesions. Both treatments with Vogt's solutions did not show any improvement.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionsupporting

confidence: 57%

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Effect of protective solutions and hydroxyethyl starch in the attenuation of the injuries of ischemia and reperfusion of splanchnic organs

et al. 2015

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“…Rats are the most commonly used experimental animal, since its splanchnic circulation has similarities to the human being's. Some extrapolation of information from one species to another may be considered 10 . While comparing the types of vascular occlusion, mesenteric artery, mesenteric vein and the occlusion of both mesenteric vessels, higher mortality of rats was reported on exclusively arterial occlusion.…”

Section: Discussionmentioning

confidence: 99%

Pentoxifylline associated to hypertonic saline solution attenuates inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats

Marques

Rasslan²,

Belon³

et al. 2014

Acta Cir. Bras.

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PURPOSE:To evaluate intestinal inflammatory and apoptotic processes after intestinal ischemia/reperfusion injury, modulated by pentoxifylline and hypertonic saline. METHODS:It was allocated into four groups (n=6), 24 male Wistar rats (200 to 250g) and submitted to intestinal ischemia for 40 min and reperfusion for 80 min: IR (did not receive any treatment); HS group (Hypertonic Saline, 4ml/kg-IV); PTX group (Pentoxifylline, 30mg/ kg-IV); HS+PTX group (Hypertonic Saline and Pentoxifylline). All animals were heparinized (100U/kg). At the end of reperfusion, ileal fragments were removed and stained on hematoxylin-eosin and histochemical studies for COX-2, Bcl-2 and cleaved caspase-3. RESULTS:The values of sO 2 were higher on treated groups at 40 minutes of reperfusion (p=0.0081) and 80 minutes of reperfusion (p=0.0072). Serum lactate values were lower on treated groups after 40 minutes of reperfusion (p=0.0003) and 80 minutes of reperfusion (p=0.0098). Morphologic tissue injuries showed higher grades on IR group versus other groups: HS (p=0.0006), PTX (p=0.0433) and HS+PTX (p=0.0040). The histochemical study showed lesser expression of COX-2 (p=0.0015) and Bcl-2 (p=0.0012) on HS+PTX group. A lower expression of cleaved caspase-3 was demonstrated in PTX (p=0.0090; PTXvsIR). CONCLUSION:The combined use of pentoxifylline and hypertonic saline offers best results on inflammatory and apoptotic inhibitory aspects after intestinal ischemia/reperfusion.

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“…The ischemia-reperfusion lesion may complicate many clinical conditions and plays an important role in the pathogenesis and survival following mesenteric blood flow reduction [3][4][5] , and an extensive research approach have been done [6][7][8][9][10] to understand the mechanisms of ischemia-reperfusion intestinal injury to obtain the best protection. The occlusion by selective clamping of the cranial mesenteric artery and the one hour ischemic time used being the routine procedure until now in most reported research but not comparatively studied until now the importance of different collateral blood supply due to anatomic variations specie dependents.…”

Section: Discussionmentioning

confidence: 99%

Ischemia-reperfusion histopathology alterations of the rabbit intestinal wall with and without exclusion of the collateral mesenteric circulation supply

Gomes¹,

Filho

Porto

et al. 2010

Acta Cir. Bras.

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Purpose:To evaluate the histopathology alterations of the intestinal mucosa of rabbits submitted to different times of mesenteric artery ischemia and reperfusion with and without celiac artery collateral circulation supply. Methods: Two groups of eight male New Zealand white rabbits (weight 2.2-3.5 kg) were used in this study. In the Group 1 animals, the proximal mesenteric artery was occluded for 60 min with an atraumatic vascular clamp, followed by reperfusion for 60 min. In the Group 2 animals the small bowel and mesentery were cut 30cm and 60cm far from the gastroduodenal pyloric transition before the proximal mesenteric artery occlusion. Small bowel biopsies were obtained before ischemia (control), after 30 min and 60 min of mesenteric ischemia and at 30 and 60 min. of mesenteric artery reperfusion. Results: In the Group I animals, the followings histopathology grade results were observed: t1, mean 0.4 + 0.29; t2, mean 1.9 ± 0.38; t3, 1.9 ± 0.33; t4, 1.2 ± 0.36 and t5, 1.2 ± 0.32. Differences between t0 and t2 and between t3 and t4 were statistically significant (p<0.05). Differences between t2 and t3 and t4 and t5 were not significant (p>0.5). In the Group II animals, it was observed: t1, mean 1.6 ± 0.33; t2, 2.4 ± 0.36; t3, 3.0 ± 0.35; t4 3.4 ± 0.31; t5, 3 ± 031. Differences between t0 and t1, t1 and t2, and t2 and t3 were significant (p<0.05). Differences between histopathology grades results of samples t1 to t5 in Group 1 and 2 were statistically significant (p<0.5). Conclusion: Microscopic examination of the biopsies revealed significant evidence of worse small bowel wall ischemiareperfusion lesions by exclusion of the celiac artery collateral circulation supply. Key words: Mesenteric Vascular Occlusion. Vascular Diseases. Splanchnic Circulation. Rabbits. RESUMOObjetivo: Avaliar as alterações histopatológicas da mucosa intestinal de coelhos submetidos à isquemia-reperfusão com e sem exclusão da circulação mesentérica colateral. Métodos: Foram estudados dois grupos de oito coelhos Nova Zelândia machos com pesos variáveis entre 2,2 e 3,5 kg de peso corpóreo. Nos animais do Grupo 1, a artéria mesentérica proximal foi ocluida por pinçamento atraumático durante 60 min, seguido de reperfusão por 60 min. No Grupo 2 o intestino delgado e o mesentério foram seccionados 30 cm e 60 após a transição pilórica gastroduodenal antes da oclusão da artéria mesentérica cranial. Biópsias da parede intestinal foram obtidas antes da isquemia (controle), após 30 e 60 min. de isquemia. Resultados: No Grupo I foram observados os seguintes graus de lesões: t1,média de 0.4 + 0.29; t2, média 1.9 ± 0.38; t3, 1.9 ± 0.33; t4, 1.2 ± 0.36 e t5, 1.2 ± 0.32. As diferenças entre t0 e t2 e entre t3 e t4 foram significantes (p<0.05). As diferenças entre t2 e t3 e t4 e t5 não foram significantes (p>0.5). No Group II observou-se: t1, média de 1.6 ± 0.33; t2, 2.4 ± 0.36; t3, 3.0 ± 0.35; t4 3.4 ± 0.31; t5, 3 ± 031. As diferenças entre t0 e t1, t1 e t2, e t2 e t3 foram significantes (p<0.05). As diferenças entre os resultados histopato...

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Systemic evaluation on ischemia and reperfusion injury of splanchnic organs in rats

Cited by 9 publications

References 18 publications

Effect of protective solutions and hydroxyethyl starch in the attenuation of the injuries of ischemia and reperfusion of splanchnic organs

Effect of protective solutions and hydroxyethyl starch in the attenuation of the injuries of ischemia and reperfusion of splanchnic organs

Pentoxifylline associated to hypertonic saline solution attenuates inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats

Ischemia-reperfusion histopathology alterations of the rabbit intestinal wall with and without exclusion of the collateral mesenteric circulation supply

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