Histological study of the liver and biochemistry of the blood of Wistar rats following ligature of right hepatic duct

Abstract

Purpose:To observe the histological alterations in the liver and biochemistry in the blood that can happen in Wistar rat, after the ligature of right hepatic duct. Methods: In this study were used rats (n=46) of Wistar pedigree. The animal groups (n=46) were distributed in 6 experimented sub-groups (n=6). It was held a ligature surgery of the right hepatic duct and euthanasia in 7, 14, 21, 28, 60 and 90 days and the biochemistry control group (n=10), that animals had 2ml of their blood taken by cardiac puncture for biochemistry study with value analyses of bilirubins, transaminasis, lactic desidrogenasis, alkaline phophatase and gamma-glutamil-transferase. Given the expected time of each group, the animals were submitted to anesthesia procedure and cavity re-opening, being held intra-cardiac puncture and with 2ml blood collected for biochemistry analyses. It was proceeded the liver resection, being the liver putted in formol solution to 10% for a period of 24 hours and taken to the histology. Results: It was not possible to identify results that express significant differences as the existence of alterations histological and biochemistrily between the different groups. Conclusion: At the end of the study, it was not possible to identify histological and biochemistrily alterations that express significant differences between livers of the animals from the right linked hepatic duct and the animals of the control group. Key words: Liver duct. Liver. Histology. Biochemistry. Rat. RESUMOIntrodução: a colestase é uma situação grave e geralmente letal. Habitualmente a obstrução do fluxo da secreção biliar ocorre por lesão iatrogênica. Cerca de 80% das lesões das vias Objetivo: Observar as alterações histológicas que possam ocorrer no fígado e bioquímicas no sangue de ratos Wistar, após ligadura do ducto hepático direito. Métodos: Neste estudo foram utilizados ratos (n=46) da linhagem Wistar. O grupo de animais (n=46) foi dividido em 2 grupos: A experimento (n=36) e B controle bioquímico (n=10), sendo o grupo A subdividido em 6 subgrupos experimento (n=6). Foi realizada cirurgia para a ligadura do ducto hepático direito e eutanásia em 7, 14, 21, 28, 60 e 90 dias. No grupo B controle bioquímico (n=10), os animais tiveram 2ml de seu sangue retirado por punção cardíaca para estudo bioquímico com análise dos valores de bilirrubinas, transaminases, desidrogenase láctica, fosfatase alcalina e gama-glutamil-transferase. Dado o prazo de espera de cada grupo, os animais foram submetidos a procedimento anestésico e reabertura da cavidade, sendo realizada punção intracardíaca, com coleta de 2ml de sangue para análise bioquímica. Foi realizada a retirada do fígado, sendo o fígado fixado em solução de formol a 10 % por um período de 24 horas e encaminhado ao laboratório de histologia. Resultados: Não foram encontrados resultados estatisticamente significantes quanto a existência de alterações histológicas e bioquímicas entre os diversos grupos. Conclusão: Ao final do estudo não se conseguiu identificar histológica e bioquim...

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