Experimental model of cultured keratinocytes

Gragnani, Alfredo; Morgan, Jeffrey R.; Ferreira, Lydia Masako

doi:10.1590/s0102-86502003001100002

Cited by 8 publications

(4 citation statements)

References 10 publications

(2 reference statements)

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“…The Improving bio-molecular understanding and therefore accelerating the development of new therapies is also an advantage of this process. The disadvantage is that after a continuous period of growth, the cellular characteristics may change and become different to that of the donor including, and it has a lack of neuroendocrine signaling in relation to experimental animals 24,25 .…”

Section: Post-burn Time Intervalmentioning

confidence: 99%

Rat an experimental model for burns: A systematic review

Mitsunaga

Gragnani

Ramos³

et al. 2012

Acta Cir. Bras.

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PURPOSE:To revise and systematize scientific knowledge of the experimental model for cutaneous burns in rats. METHODS:A bibliographical review from 2008 up to January 2011 in PubMed, EMBASE and LILACS was undertaken. Were used the keywords: animal models, burns and rats. 221 studies were identified, and 116 were selected. RESULTS:It was found that: 54/86 (62.7%) had third degree burns; 55/73 (75.3%) studied the back; 45/78 (57.6%) used heated water and 27/78 (35.9%) incandescent instruments; 39/78 (50%) studied systemic effects; 22/71 (31%) used ketamine associated with xylazine; 61/64 (95.3%) performed depilation with appropriate equipment; 36/72 (50%) used microscopy; more than 50% did not describe analgesia or antibiotics during the postoperative period; in 42/116 (36.2%) postoperative fluid therapy was performed; and the time interval after the burn, up to the beginning of the results analysis varied from 7s up to four weeks. Legislation issues on burn experiments are discussed. CONCLUSION:The hot water was the main method to induce burns those of third degree on the back, with anesthesia using ketamine and xylazine, after depilation. These were evaluated microscopically, without using analgesia or an antibiotic during the postoperative period. The studies were not very reproducible. CONCLUSÃO: Líquido aquecido foi o principal método para induzir queimadura de terceiro grau no dorso do animal, com anestesia usando quetamina e xilazina, após depilação, avaliados por microscopia, sem uso de analgesia ou antibióticos. Os estudos não são reprodutíveis.Descritores: Modelos Animais. Queimaduras. Pele. Revisão. Ratos.Mitsunaga Jr JK et al.

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Section: Post-burn Time Intervalmentioning

confidence: 99%

Rat an experimental model for burns: A systematic review

Mitsunaga

Gragnani

Ramos³

et al. 2012

Acta Cir. Bras.

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“…The composite grafts constituted by human acellular dermis seeding with primary cultured keratinocytes and elevated to the air-liquid interface on the third day after seeding and on the tenth day the grafts are transplanted to the back of the mice 10, 12 .…”

Section: Methods Descriptionmentioning

confidence: 99%

Experimental model of cultured skin graft

2004

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-One of the most used animal models of cultured keratinocytes autografting is based on xenografting of human keratinocytes to the rat or athymic mice, immunological neutral recipient that acts as biological carrier. It could be studied in this model many facts that occur after transplant without the ethical aspect in the clinical study. The proposition of the experimental model is related to the sequence of the total or partial skin transplant, as autografting or xenografting, cultured or not, to the back of athymic mice. The model presents the possibility of study in vivo athymic animal, when the in vivo study in anima nobili is not ethical. It permits the xenografting evaluation of cultured cells graft or of the genetically modified cells and of the association of the cultured cells and the dermal substitutes, the composite grafts, and of the autografting.

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“…A aplicabilidade desse modelo experimental em Cirurgia Plástica, por meio da cultura de queratinócitos, tem permitido a realização de diversos estudos sobre a cicatrização e seus distúrbios. 29 O modelo de cultura de fibroblastos de quelóides tem sido utilizado para análise do comportamento bioquímico, e da ação de fatores de crescimento sobre estas lesões, comparando-se com fibroblastos de derme normal. 30,31 Culturas de células provenientes de neoplasias cutâneas também vêm sendo aplicadas no estudo do comportamento destes tumores.…”

Section: Cultura De Células Tecidos E óRgãos (Modelos In Vitro)unclassified

Modelos experimentais em pesquisa

2005

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The purpose of this study is to collect the main classifications of experimental research models and their possible applications in experimental research. Literature search was done using the most important data bank available on the internet (PUBMED, MEDLINE, SCIELO, LILACS, COCHRANE). A compilation of the experimental studies developed at the Plastic Surgery Post Graduate Program of the Federal University of São Paulo/Paulista School of Medicine was also done. Animals models were classified regarding sanitary and genotypical status. The understanding of the main classifications of the research experimental models is essential to improve and confirm procedures and techniques already described as well as the development of new ones.

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Experimental model of cultured keratinocytes

Cited by 8 publications

References 10 publications

Rat an experimental model for burns: A systematic review

Rat an experimental model for burns: A systematic review

Experimental model of cultured skin graft

Modelos experimentais em pesquisa

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