Uric acid as a monitor of oxidative stress in a random skin flap in rats

Abstract

PURPOSE: To verify the possible role of uric acid as a monitor of oxidative stress in a random skin flap, in the rat. METHODS: This study utilized 21 adult male Wistar-EPM rats divided, at random, in three groups of seven animals, to evaluate the behavior of uric acid levels during oxidative stress zero, 5 and 15 minutes after elevation of the flap. According to the time schedule, skin samples were collected in order to allow determination of uric acid and malondialdehyde (MDA) levels. RESULTS: Uric acid levels in the skin samples did not change with time, but the values in normal skin were higher since the beginning. Malondialdehyde (MDA) levels in the skin samples remained stable throughout. CONCLUSION: Uric acid levels in the flap skin were lower than those in the normal skin, denoting local oxidative stress.

Effect of alpha lipoic acid co‐administration on structural and immunohistochemical changes in subcutaneous tissue of anterior abdominal wall of adult male albino rat in response to polypropylene mesh implantation

“…However, in rats and in other loose‐skinned animals, a thin sheet of panniculus carnosus muscle separates the subcutaneous tissue layer from the dermis (Freire et al . ). In the present study, the structure of the subcutaneous layer in the control group was similar to that described in other literature (Kawamata et al .…”

“…In human and in other fixed-skinned species, the subcutaneous layer is considered a deeper continuation of the dermis with a variable amount of fat cells and collagen fibres. However, in rats and in other loose-skinned animals, a thin sheet of panniculus carnosus muscle separates the subcutaneous tissue layer from the dermis (Freire et al 2003). In the present study, the structure of the subcutaneous layer in the control group was similar to that described in other literature (Kawamata et al 2003).…”

Modelos experimentais em pesquisa

“…16 Também existem pesquisas da utilização desse modelo experimental no estudo de alterações bioquímicas seqüenciais do estresse oxidativo sobre o retalho. 17,18…”

Vitamins E and C Concentrations in Anaemia Subjects Attending Braithwaite Memorial Specialist Hospital Port Harcourt

“…Vitamin C, also known as ascorbic acid, is a water soluble vitamin unlike most mammals; humans do not have the ability to make their own vitamin C. Hence, Vitamin C has to be supplemented through diet. It has been reported that Vitamin C is involved in the metabolism of cholesterol to bile acids, which may have implication for blood cholesterol levels and the incidence of gallstones [3]. It has been reported that smokers have lower blood levels of vitamin C. this has been attributed to increased oxidative stress from common cigarette smoke [4].…”