A comparação entre o transplante de células tronco mononucleares e mesenquimais no infarto do miocárdio

Guarita-Souza, Luiz César; Carvalho, Katherine Athayde Teixeira de; Rebelatto, Cármen Lúcia Kuniyoshi; Senegaglia, Alexandra Cristina; Hansen, Paula; Furuta, Marcos; Miyague, Nelson Itiro; Francisco, Júlio César; Olandoski, Márcia; Woitowicz, Vinícius; Simeoni, Rossana Baggio; Faria-Neto, José Rocha; Brofman, Paulo

doi:10.1590/s0102-76382005000300007

Cited by 16 publications

(10 citation statements)

References 23 publications

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“…The heart is exposed through a left thoracotomy incision and ligation of the proximal coronary artery is performed with 7-0 yarn [13,14]. Thus, it is an experimental model more complex, that beyond the need for thoracotomy is associated with ventilatory support and postoperative analgesia.…”

Section: Discussionmentioning

confidence: 99%

Modelo experimental de infarto do miocárdio induzido por isoproterenol em ratos

Filho¹,

Ferreira²,

Sousa³

et al. 2011

Rev Bras Cir Cardiovasc

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Objective: To evaluate and validate, in our laboratory, the essay of myocardial infarction induced by isoproterenol in rats by means of analysis of hematological, biochemical, oxidative stress markers and histopathological parameters.Methods: Thirty young, male, Wistar rats (145 to 230 g) were randomly allocated in two groups: Sham group, which underwent a virtual myocardial infarction induction, and the Infarction group, which underwent a myocardial infarction induction with isoproterenol. The administrations for the infarction induction were performed during two consecutive days and a 24-hour interval between them. Twenty-four hours after the last administration, rats from both groups were anesthetized and sacrificed for blood sample collection to evaluate complete blood count (CBC) and biochemical parameters (SGOT, SGPT, troponin I, urea and creatinin), obtain myocardial fragments for oxidative stress markers analyses (catalase activity and glutathione concentrations) as well as histopathological examinations.Results: There were no death cases in the Sham group, while the mortality rate in the Infarction group was 25%. Myocardial infarction induction with isoproterenol raised leukocytes and neutrophils counts, SGOT, troponin I and urea concentrations, reduced catalase enzyme activity and glutathione concentrations in the myocardium and let to histopathological concentrations as well. It did not exert alterations in terms of hemoglobin, SGPT and creatinin concentrations.Conclusion: The isoproterenol-induced myocardial infarction essay in rats was adequately reproduced in our laboratory, causing alterations in hematological, biochemical, oxidative stress markers and histopathological parameters. Rev Bras Cir Cardiovasc 2011;26(3):469-76 In the literature, there is a series of studies using this model, evaluating the effect of different substances on biochemical, oxidative stress markers and histopathological damage resulting from myocardial in rats [8,9]. DescriptorsThis study aims to evaluate and validate, in our environment, the model of isoproterenol inducedmyocardial infarction in rats, given the scarcity of scientific publications using this model in our country METHODS This study, whose data are from a Master

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Section: Discussionmentioning

confidence: 99%

Modelo experimental de infarto do miocárdio induzido por isoproterenol em ratos

Filho¹,

Ferreira²,

Sousa³

et al. 2011

Rev Bras Cir Cardiovasc

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“…In the control group, as a result of deterioration of ventricular function after AMI, it already was expected that ventricular remodeling would happen. However, the sedentary group with cells showed statistically significant ventricular dilation, which was also identified in a study published by the same group [14]. Regarding the trained group with cells, although ventricular dilation is identified, it was considered that there was stabilization of ventricular remodeling.…”

Section: Discussionmentioning

confidence: 59%

“…It is believed that factors released due to paracrine effect of stem cells can promote reduction of the fibrosis area, suggesting recovery of left ventricular function, which can be corroborated with improved LVEF and limiting ventricular remodeling. These two mechanisms may also be explained by the angiogenic potential of bone marrow mononuclear stem cells, previously described in other studies and the mechanisms of vasodilation produced by physical activity [14,15].…”

Section: Discussionmentioning

confidence: 59%

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Effect of exercise associated with stem cell transplantation on ventricular function in rats after acute myocardial infarction

Cosmo

Francisco

Cunha

et al. 2012

Revista Brasileira De Cirurgia Cardiovascular

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Objective: To assess the functional and anatomicalpathological effect of transplantation of bone marrow mononuclear cells associated to aquatic physical activity after myocardial infarction in rats.Methods: Twenty-one rats were induced by myocardial infarction, through left coronary artery ligation. After a week, the animals were subjected to echocardiography for evaluation of left ventricle ejection fraction (LVEF, %) and dyastolic and end systolic volume of the left ventricle

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“…The first study using the mononuclear fraction appeared in cardiology and showed promising results, encouraging the use of these cells as a treatment in other clinical situations (Guarita‐Souza et al, 2005; Martino et al, 2006). Caldas et al (2008 studied the utilization of mononuclear and mesenchymal cells in a renal failure model using Wistar rats.…”

Section: Introductionmentioning

confidence: 99%

Microscopic evidences that bone marrow mononuclear cell treatment improves sciatic nerve regeneration after neurorrhaphy

Lopes-Filho

Caldas

Santos

et al. 2010

Microscopy Res & Technique

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Cell therapy constitutes a possibility for improving nerve regeneration, increasing the success of nerve repair. We evaluate the use of mononuclear cells in the regeneration of the sciatic nerve after axotomy followed by end-to-end neurorrhaphy. Forty adult male Wistar rats (250-300 g) were divided into four groups: (1) sham, (2) neurorrhaphy: the sciatic nerve was sectioned and repaired using epineural sutures, (3) culture medium: after the suture, received an injection of 10 μL of culture medium into the nerve, and (4) mononuclear cell: after the suture, a concentration of 3 × 10(6) of mononuclear cell was injected in epineurium region. Mononuclear cells were obtained from the bone marrow aspirates and separated by Ficoll-Hypaque method. The histological analyses were performed at the 4th postoperative day. The sciatic functional index, histological, and morphometric analyzes were used to evaluate nerve regeneration at the 6th postoperative week. Six rats were used for immunohistochemical analysis on the 4th postoperative day. In the group 4, on the fourth day, the histological analysis demonstrated a more accelerated degenerative process and an increase of the neurotrophic factors was observed. In the 6th week, all the morphometric results of the group 4 were statistically better compared with groups 2 and 3. There was a statistically significant improvement in the sciatic functional index for group 4 compared with groups 2 and 3. Mononuclear cells stimulated nerve regeneration, most probably by speeding up the Wallerian degeneration process as well as stimulating the synthesis of neurotrophic factors.

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A comparação entre o transplante de células tronco mononucleares e mesenquimais no infarto do miocárdio

Cited by 16 publications

References 23 publications

Modelo experimental de infarto do miocárdio induzido por isoproterenol em ratos

Modelo experimental de infarto do miocárdio induzido por isoproterenol em ratos

Effect of exercise associated with stem cell transplantation on ventricular function in rats after acute myocardial infarction

Microscopic evidences that bone marrow mononuclear cell treatment improves sciatic nerve regeneration after neurorrhaphy

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