Transplante celular: análise funcional, imunocitoquímica e histopatológica em modelo experimental de miocardiopatia isquêmica utilizando diferentes células

Abstract

Objective: To present the functional, immunocytochemical and histopathologic results (in vitro or in heart specimens) after isolation, culture and co-culture of mesenchymal stem cells and skeletal myoblast cells transplanted and cotransplanted in experimental animals with ischemic heart disease and left ventricular ejection fractions lower than 40%. Methods: We utilized 72 Wistar rats, divided into four groups according to the culture media or injected cells: control group into which only culture media was injected (22 rats); mesenchymal stem cell group (17 rats); myoblast skeletal cell group (16 rats) and co-culture group (17 rats). In the immunohistochemical studies, the cells were stained with anti-vimentin, anti-desmin and anti-myosin. In the histopathologic analysis, slides were stained with Gomori Trichrome, and the neo-vessels and muscle tissues were identified. In the functional analysis the left ventricle ejection fraction was analyzed one week after myocardial infarction and one month after the injection. Results:The initial left ventricle ejection fraction (control echo) was not statistically significant between the four groups (P=0.276), but was significantly different in the follow-up examination (P=0.001). This difference was seen between the control and myoblast skeletal cell groups (P=0.037), between the control and co-culture groups (P<0.001) and between the mesenchymal stem cell and co-culture groups (P=0.025). When the initial and final echocardiograms in each group were compared, the control group deteriorated (P=0.005) and the co-culture group improved (P=0,006). With the immunocytochemical in vitro analysis, mesenchymal stem cells were identified when stained with anti-vimentin and muscle cells when stained with anti-desmin. In the heart specimens, muscle tissue, stained with anti-desmin and skeletal myoblast cells, stained with fast anti-myosin were identified. In the histopathologic analysis, new vessels were observed in the mesenchymal stem cell and skeletal myoblast groups, and muscle tissue, angiogenesis and myogenesis in the co-culture group. Transplante celular: análise funcional, imunocitoquímica e histopatológica em modelo experimental de miocardiopatia isquêmica utilizando diferentes células

Modelo experimental de infarto do miocárdio induzido por isoproterenol em ratos

“…The heart is exposed through a left thoracotomy incision and ligation of the proximal coronary artery is performed with 7-0 yarn [13,14]. Thus, it is an experimental model more complex, that beyond the need for thoracotomy is associated with ventilatory support and postoperative analgesia.…”

A Utilização de Células-tronco em Doenças Cardíacas: Apresentação dos Estudos Publicados no Brasil

“…No estudo realizado por Brofman et al (2004) os efeitos da terapia celular foram avaliados em ratos Wistar com miocardiopatia isquêmica e fração de ejeção do ventrículo esquerdo menor de 40%. Os animais foram divididos em grupo controle que recebeu apenas o meio de cultura, grupo de células-tronco mononucleares da medula óssea, grupo de células mioblásticas esqueléticas e grupo co-cultura de células-tronco mononucleares e mioblásticas esqueléticas.…”

Substituição valvar mitral com papilopexia cruzada e constrição anular em pacientes com insuficiência cardíaca

“…Thus, the search for other methods of surgical treatment remains constant on the part of researchers who are dedicated to treating this serious disease [6][7][8][9][10][11][12][13]. A new possibility then arose: surgically approaching the mitral valve in patients with severe left ventricular dysfunction in order to improve ventricular performance.…”