Antiacetylcholinesterase and antioxidant activity of essential oils from six medicinal plants from Burkina Faso

Abstract

Abstract:In this investigation, we evaluated essential oils from six medicinal plants from Burkina Faso for their antiacetylcholinesterase and antioxidant abilities. The chemotype of most active were also determined. The best antiacetylcholinesterase activities were recorded for the essential oils of Eucalyptus camaldulensis (IC50 18.98 µg/mL) and Ocimum canum (IC50 36.16 µg/mL). Their chemotype have been related to the 1,8-cineole one. Both essential oils demonstrated a linear mixed non competitive inhibition. The essential oil of Ocimum basilicum which belong to the linalool-eugenol chemotype exhibited the best radical scavenging activity (IC50 3.82 µg/mL) and reducing power (531.75 mg AAE/g). In comparison with gallic and ascorbic acids, O. basilicum essential oil evidenced interesting antioxidant activities. The antiacetylcholinesterase and antioxidant activities of essential oils were discussed in regard with their chemical composition.

Antioxidant and anticholinesterase activities of the essential oil of Eugenia dysenterica DC.

“…The reaction is monitored for 5 min at 412 nm and the initial velocity (V0) recorded. Anticholinesterase activity (%) was calculated using Equation 1 (Kiendrebeogo et al, 2011): Sample V0 and blank V0 represents the initial velocities of samples and blank. Inhibition concentration of 50% (IC50) values are obtained using Log-Probit.…”

“…The best anti-AChE activity was found for EOED (IC 50 = 0.92 µg.ml ) (Kiendrebeogo et al, 2011). When starting the Ellman assay, it was observed that EOC was able to inhibit the enzyme AChE with an IC 50 = 0.92 μg.ml -1 (Means from independent experiments were then expressed as means ± standard deviation (SD).…”

Anti-acetylcholinesterase and antioxidant activities and HPLC-MS analysis of polyphenol from extracts of Nelsonia canescens (Lam.) Spreng.

“…AChE inhibitory assay, with inhibition kinetics analysis, was conducted according to the protocol described by Kiendrebeogo et al with some modifications [16] . A volume of 50 µL of Tris-HCl buffer (50 mmol/L, pH 8, 0.1% BSA) was added with 25 µL of extract (final concentration of 100 µg/ mL dissolved in MeOH buffer 10%) and 25 µL of AChE (0.22 U/mL).…”

Potential biological efficacy of Pinus plant species against oxidative, inflammatory and microbial disorders

“…Phytochemically, it is reported to contain constituents like terpenoids, flavonoids, tannins, and xanthones. The resin is composed of car-3-ene, pinene, longifolene, camphene, limonene, α-terpinene, α-terpineol, d-borneol and dl-camphor [ 19 , 20 ]. P. wallichiana is exploited for timber and used for the production of turpentine oil, rosin, needle oil and camphor [ 21 , 22 ].…”