Anti-acetylcholinesterase and antioxidant activities and HPLC-MS analysis of polyphenol from extracts of Nelsonia canescens (Lam.) Spreng.

Ouattara, Nabèrè; Meda, Roland Nâg-Tiéro; Hilou, Adama; Guenné, Samson; Konaté, Kiessoun; Coulibaly, Abou; Kiendrebéogo, Martin; Millogo, Jeanne; Nacoulma, Odile Germaine

doi:10.1016/s2222-1808(13)60088-2

Cited by 15 publications

(16 citation statements)

References 20 publications

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“…Without any doubt, hesperidin, present in most of the Citrus extracts screened in the HPTLC analysis, could be one of the compounds contributing somewhat to BChE inhibitory eff ects of the extracts. Caff eic acid, a dominant phenolic compound available in many Citrus cultivars (25,26), was reported earlier to exhibit either low inhibitory activity ((11.05±1.03) % at 100 μg/mL) against AChE (27) and IC 50 ≥200 μM (28) or no inhibition (29), which is consistent with our result ((6.01±2.50) % at 100 μg/mL).…”

Section: Hptlc Profi Le Of the Extractssupporting

confidence: 93%

HPTLC Finger-Printing and Cholinesterase Inhibitory and Metal-Chelating Capacity of Various Citrus Cultivars and Olea europaea

Şenol

Ankli²,

Reich³

et al. 2016

Food Technol. Biotechnol.

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SummaryInhibitory activity of thirty-one ethanol extracts obtained from albedo, fl avedo, seed and leaf parts of 17 cultivars of Citrus species from Turkey, the bark and leaves of Olea europaea L. from two locations (Turkey and Cyprus) as well as caff eic acid and hesperidin was tested against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), related to the pathogenesis of Alzheimer's disease, using ELISA microtiter assays at 500 μg/mL. Metal-chelating capacity of the extracts was also determined. BChE inhibitory eff ect of the Citrus sp. extracts was from (7.7±0.7) to (70.3±1.1) %, whereas they did not show any inhibition against AChE. Cholinesterase inhibitory activity of the leaf and bark ethanol extracts of O. europaea was very weak ((10.2±3.1) to (15.0±2.3) %). The extracts had either no or low metal-chelating capacity at 500 μg/mL. HPTLC fi ngerprinting of the extracts, which indicated a similar phytochemical patt ern, was also done using the standards of caff eic acid and hesperidin with weak cholinesterase inhibition. Among the screened extracts, the albedo extract of C. limon 'Interdonato', the fl avedo extracts of 'Kara Limon' and 'Cyprus' cultivars and the seed extract of C. maxima appear to be promising as natural BChE inhibitors.

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Section: Hptlc Profi Le Of the Extractssupporting

confidence: 93%

HPTLC Finger-Printing and Cholinesterase Inhibitory and Metal-Chelating Capacity of Various Citrus Cultivars and Olea europaea

Şenol

Ankli²,

Reich³

et al. 2016

Food Technol. Biotechnol.

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“…ABTS radical scavenging capacity of extracts was measured by using the method of Ouattara with some modifications [42]. In specific, ABTS powder was dissolved in distilled water to make 7 mM solution and then mixed with 2.45 mM of potassium persulfate solution.…”

Section: Methodsmentioning

confidence: 99%

Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components

Wang

Huang

et al. 2019

Molecules

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We aimed to purify polyphenols from distiller’s grain extract using macroporous resins and to identify its polyphenolic components. The influence of operational parameters on purification efficiency was investigated. The polyphenolic composition was analyzed by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) and then quantified by UPLC-MS using authenticated standards. The results showed that the optimal purifying conditions were D101 resin with a dosage of 3 g, four hours adsorption, three hours desorption time, and 60% ethanol as the eluent, producing the highest purification rate of 51%. The purified distiller’s grain extract exhibited stronger antioxidant activity than the unpurified extracts, which was assessed using DPPH and ABTS methods (IC50 DPPH = 34.03 and 16.21 μg/mL, respectively; IC50 ABTS = 20.31 and 5.73 μg/mL, respectively). UPLC-MS results indicated that (−)-epicatechin is the major compound found in distiller’s grain extract which was quantified as 562.7 μg/g extract, followed by ferulic acid (518.2 μg/g), p-hydroxybenzoic acid (417.7 μg/g), caffeic acid (217.1 μg/g), syringic acid (158.0 μg/g) and quercetin (147.8 μg/g). Two compounds, vanillic acid (66.5 μg/g) and gallic acid (41.4 μg/g), were found in lower concentrations. The findings of this study suggest that purification of polyphenolic compounds from distiller’s grain by macroporous resins is feasible, providing a new and effective method for the secondary use of distiller’s grain resources.

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“…These two different studies using methanol extracts show that the most anticancer compounds in this medicinal plant were extractible with polar solvent including methanol. Interesting, in previous phytochemical investigations by HPLC-MS using methanol extract of this plant, we revealed the presence of five phenol acids (p-coumaric acid, caffeic acid, chlorogenic acid, ferulic acid and gentisic acid) and three flavonoids (apigenin, luteolin, Quercetol) [14]. Numerous researches noted the anticancer potential of these various molecules from herbal.…”

Section: Resultsmentioning

confidence: 75%

“…Previous studies of Nelsonia canescens methanol extract showed a good anti-tumor activity on breast-cancer cell line U373 with a IC 50 of 27.04 ± 1.55 µg/mL [13] and Phytochemical investigations by HPLC-MS revealed the presence of five phenol acids (p-coumaric acid, caffeic acid, chlorogenic acid, ferulic acid and gentisic acid) and three flavonoids (apigenin, luteolin, Quercetol) [14]. However, this plant species is widely used traditionally in the treatment of cervical cancer, but little scientific evidence supports its use.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity Potential of Nelsonia canescens (Lam.) Spreng Extracts against Cervical Cancer Cell Lines

Ouattara¹,

Alphonsine²,

Guenné³

et al. 2019

SJBR

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Nelsonia canescens is a small perennial herb widely used in Burkina Faso traditional medicine for the treatment of any diseases. In the present study, the purpose was to investigate the potential cytotoxicity and selectivity of different extracts from N. canescens on human cervical cancer HeLa and SiHa cell lines. MTT assay revealed higher cytotoxic activity of methanol extract in a dose-dependent manner against both HeLa and SiHa cells with respective IC 50 values of 21.72 ± 0.08 g/mL and 25.30 ± 0.44 g/mL. Methanol extract (ME) showed the best selectivity index value of 7.93 ± 0.03 with HeLa cell line and Ethyl acetate extract (EAE) showed the best selectivity index value of 7.48 ± 0.05 with SiHa. The potential cytotoxicity and selectivity of N. canescens extracts against HeLa and SiHa cells line suggest that this species could be exploited further as a potential lead in cervical cancer treatment.

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Anti-acetylcholinesterase and antioxidant activities and HPLC-MS analysis of polyphenol from extracts of Nelsonia canescens (Lam.) Spreng.

Cited by 15 publications

References 20 publications

HPTLC Finger-Printing and Cholinesterase Inhibitory and Metal-Chelating Capacity of Various Citrus Cultivars and Olea europaea

HPTLC Finger-Printing and Cholinesterase Inhibitory and Metal-Chelating Capacity of Various Citrus Cultivars and Olea europaea

Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components

Cytotoxicity Potential of Nelsonia canescens (Lam.) Spreng Extracts against Cervical Cancer Cell Lines

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