Antioxidant activity from the leaf extracts of Jacaranda puberula Cham., Bignoniaceae, a Brazilian medicinal plant used for blood depuration

Abstract

RESUMO: "Atividade antioxidante do extrato das folhas de Jacaranda puberula Cham., Bignoniaceae, uma planta medicinal brasileira usada como depurativo do sangue". Os extratos obtidos a partir das folhas de Jacaranda puberula Cham., Bignoniaceae, foram avaliados quanto à atividade antioxidante utilizando-se o método do radical livre DPPH (2,2-difenil-1-picrilhidrazila). Também foram incluídos na avaliação três medicamentos fitoterápicos comerciais (F1, F2 e F3) indicados como depurativos do sangue. Através da descoloração das soluções testadas, foi possível medir o potencial sequestrador do radical DPPH. Como referência, utilizou-se os dados da literatura de CE 50 do extrato padronizado de Gingko biloba EGb 761 ® e o flavonoide rutina, ambos empregados em medicamentos com atividade antioxidante. Entre os extratos testados, os que apresentaram valores baixos de CE 50 foram os extratos etanólico bruto (EE), em acetato de etila (EA), em butanol (EB), aquoso (EAq) e da amostra A, obtida do extrato EB. Os fitoterápicos comerciais apresentaram altos valores de CE 50 . Com base nos resultados das análises feitas por de RMN de 13C e 1 H, CLAE-DAD e de acordo com os dados da literatura, a presença de substâncias fenólicas como o verbascosídeo (1) e o aldeído cis-caféico (2), contribuíram para a atividade antioxidante (AA) dos extratos ativos.Unitermos: Jacaranda puberula, Bignoniaceae, depurativo, atividade antioxidante, verbascosídeo, aldeído cis-caféico. ABSTRACT:The antioxidant activity of leaf extracts from Jacaranda puberula Cham., Bignoniaceae, was assayed by the DPPH (2,2-diphenyl-1-picryl-hydrazyl) free radical scavenging method. Three phytomedicines (F1, F2, and F3) used as blood depurative, were tested by the same method. The free radical scavenger potential was measured by the discoloration of the solution. The EC 50 values from Gingko biloba EGb 761 ® extract and rutin, used as antioxidant for medical purposes, were used as reference. The ethanol extract (EE), ethyl acetate (EA), butanol (EB), aqueous (EAq) and the sample A (obtained from extract EB), showed lower EC 50 values than other extracts and phytomedicines. The antioxidant activity (AA) of the extracts was related with the presence of the polyphenol compounds such as verbascoside (1) and cis-caffeoyl aldehyde (2). These structures were determined by chemical and spectroscopic methods and comparison with literature data.

Antioxidant and Antiulcerogenic Activity of the Dry Extract of Pods of Libidibia ferrea Mart. ex Tul. (Fabaceae)

“…Antioxidants protect the body against the oxidative damage involved in various diseases, which are believed to be caused or related to excess free radicals in the body, including cancer, hypertension, heart disease, and diabetes [72, 73]. Oxidative stress has a role in the etiology of gastrointestinal diseases.…”

Biological properties and phytochemical characterization from Miconia chamissois Naudin aqueous extract

“…Phenolic compounds such as flavonoids have been reported to promote antioxidant activity by reducing free radicals and by chelating metals, mechanisms also involved in inflammatory processes (Coutinho et al, 2009;Tarawneh et al, 2014). Compounds of vegetal origin with antioxidant properties have been highlighted by their potential to treat degenerative diseases that involve oxidative processes, such as heart disease, cancer, and changes in DNA (Santos et al, 2010;Silva et al, 2012). Oxidative processes are mechanisms that occur in the normal functioning of the body, being reactive species of oxygen and nitrogen generated during cellular metabolism.…”

“…However, the presence of an excess of these compounds has been related to the development of diseases such as atherosclerosis, changes in DNA, changes in proteins, changes in enzymes, and their effect on inflammatory processes (Barreiros et al, 2006). Polyphenols have been reported to possess the ability to reduce free radicals, for example, by sequestering OH groups (Santos et al, 2010). Therefore, there has been an increasing interest in the study of plants that have phenolic compounds and antioxidant activity.…”

Comparison of the phytochemical profiles of five native plant species in two different forest formations

“…Same authors confirmed the presence of steroids, triterpenes and saponins in the genus Jacaranda. Subramanian et al (1973) also found the phenol hydroquinone in Jacaranda mimosaefolia, while Santos et al (2010a) found several types of aldehydes and polyphenols in Jacaranda puberula.…”