Avaliação da atividade antimicrobiana e citotóxica de extratos de Gochnatia polymorpha ssp floccosa

Abstract

RESUMO:Neste estudo procedeu-se a avaliação da atividade antimicrobiana e citotóxica de extratos de Gochnatia polymorpha ssp fl occosa, espécie empregada na medicina popular contra doenças respiratórias. Folhas, cascas do tronco e ramos foram extraídos com hexano, diclorometano e etanol, sucessivamente, sendo obtidos os respectivos extratos brutos. A atividade antimicrobiana foi determinada pelo método de difusão em ágar utilizando-se bactérias ABSTRACT: "Evaluation of antimicrobial and cytotoxic activitiy of extracts from Gochnatia polymorpha (Less) ssp fl occosa". The antimicrobial and cytotoxic activities of Gochnatia polymorpha ssp fl occosa, a medicinal plant used against respiratory diseases, were evaluated. Successive petroleum ether, dichloromethane and ethanol extracts of dried leaves, trunk bark and stems were used in the brine shrimp lethality bioassay and tested against 22 strains of microorganisms (Gram-positive and Gram-negative bacteria and fungi) by the well-diffusion agar method. None of the extracts showed cytotoxicity on the brine shrimp bioassay. The extracts of leaves showed a mild activity against some strains of Staphylococcus aureus and Streptococcus mutans. The ethanol extracts of trunk bark and dichloromethane extract of stems also showed activity against Micrococcus luteus, Staphylococcus aureus and S. epidermidis. The highest activity was found in the dichloromethane extract of trunk bark, which showed signifi cant antibacterial and antifungal activities against Micrococcus luteus, Staphylococcus aureus, S. epidermidis, Streptococcus mutans, Enterococcus faecalis and Candida albicans. Diterpenes are present in this extract and could be responsible for the activity. All the screened extracts were shown to be inactive (at 5.0 mg/mL) against strains of Gram-negative bacteria tested. These results indicate the potential of G. polymorpha ssp fl occosa as a source of antibacterial compounds and partly support the use of this plant in folk medicine.

Brine shrimp bioassay of some species of Solanum from Northestern Brazil

“…The brine shrimp assay has been established as a safe, practical, and economic method for the determination of the bioactivity of synthetic compounds (Almeida et al, 2002) as well as plant products (Meyer et al, 1982;McLaughlin et al, 1991;Lhullier et al, 2006;Stefanello et al, 2006). The signifi cant correlation between the brine shrimp assay and in vitro growth inhibition of human solid tumor cell lines demonstrated by the National Cancer Institute (NCI, USA) is signifi cant because it shows the value of this bioassay as a pre-screening tool for antitumor drug research (Anderson et al, 1991).…”

Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansing

“…The research was a study that involved patent application delaying its disclosure, so it was used Artemia test, which is considered a preliminary study of low cost and easy handling on bioassay of extracts with strong biological activity [19], since the accomplishment of the lethality test allows the evaluation of the toxicity involving only one parameter: life or death [20].…”

Cytotoxicity of Wedelia paludosa D.C. extracts and constituents

“…The brine shrimp lethality bioassay (BSLB) has been used as an efficient, rapid and inexpensive front-line test for the detection of bioactive compounds (Meyer et al, 1982;Ghisalberti, 1997;Lhullier et al, 2006;Stefanello et al, 2006;Silva et al, 2007;Nunes et al, 2008;Shoeb et al, 2008;Subhan et al, 2008). This bioassay requires small amount of sample (Solis et al, 1993), and it generally correlates well with cytotoxic (McLaughlin et al, 1998) and trypanosomicidal (Zani et al, 1995) activities.…”