Cytotoxicity of Wedelia paludosa D.C. extracts and constituents

Abstract

RESUMO: "Citotoxidade de extratos e constituintes de Wedelia paludosa D.C." Wedelia paludosa D.C. (Asteraceae) é uma planta ornamental facilmente encontrada em várias regiões do Brasil, principalmente nos estados de Santa Catarina, São Paulo, Minas Gerais, Bahia e Pernambuco. Objetivando descobrir novas substâncias citotóxicas a partir desta espécie, o extrato hidrometanólico de W. paludosa (HME) e as frações diclorometânica (FD) e aquosa (FA) resultantes de sua partição em CH 2 Cl 2 -H 2 O foram avaliados utilizando-se o bioensaio em Artemia salina. A fração diclorometânica (FD) apresentou a maior atividade citotóxica (CL 50 = 140,6 mg/mL), e sua análise por cromatografia líquida de alta eficiência empregando-se fase reversa (FR-CLAE) revelou os ácidos caurenóico (1, 6,22 ± 0,23%) e grandiflorênico (2, 3,22 ± 0,31%) como constituintes majoritários. As amostras HME (CL 50 = 980 mg/mL), FD (CLC 50 = 140,6 mg/mL), 1 (CL 50 = 15,9 mg/mL) e 2 (CL 50 = 29,8 mg/mL) foram citotóxicas contra A. salina, enquanto que a fração aquosa (FA, CL 50 >> 1000 mg/mL) mostrou-se inativa. Conclui-se que a citotoxidade observada para HME e FD pode ser atribuída à presença dos ácidos caurenóico (1) e grandiflorênico (2) nestes extratos.Unitermos: Wedelia paludosa, Asteraceae, atividade citotóxica, toxidade em Artemia salina, ácido caurenóico, ácido grandiflorênico, CLAE.ABSTRACT: Wedelia paludosa D.C. (Asteraceae) is an ornamental species occurring in many regions of Brazil. Aiming to find new cytotoxic compounds, the hydromethanol extract of W. paludosa (HME), as well as the dichloromethane (DF) and water (WF) fractions resulting from its partition, were submitted to the brine shrimp lethality bioassay (BSLB) in order to evaluate their cytotoxicity. Dichloromethane fraction (DF) was shown to be the most cytotoxic fraction (LC 50 = 140.6 mg/mL), and its analysis by reversed phase high performance liquid chromatography (RP-HPLC) revealed ent-kaurenoic (1, 6.22 ± 0.23%) and grandiflorenic (2, 3.22 ± 0.31%) acids as important constituents. HME (LC 50 = 980 mg/mL), DF (LC 50 = 140.6 mg/mL), 1 (LC 50 = 15.9 mg/ mL) and 2 (LC 50 = 29.8 mg/mL) were found to be cytotoxic, while the water fraction (WF, LC 50 >> 1000 mg/mL) was inactive. As conclusion, the cytotoxicity observed for HME and DF is mainly due to the presence of 1 and 2 in their constitution.

Biotransformation of 4-nerolidylcatechol by Heraclides brasiliensis (Lepidoptera: Papilionidae) reduces the toxicity of Piper umbellata (Piperaceae)

“…The BSLB was performed according to the procedure proposed by Batista et al (2009). Brine shrimp eggs were hatched in artificial seawater (40 g/L, sea salt).…”

In vitro cytotoxic and genotoxic effects of Cissus verticillata and Sphagneticola trilobata used for treatment of Diabetes Mellitus in Brazilian folk medicine

“…This plant is popularly known as "Pseudo-arnica, Margaridão, Pingo- de-ouro, Arnica-do-mato, Picão-da-praia, and Vedélia" and can be found in many regions of Brazil. Despite being considered an ornamental plant owing to the presence of yellow flowers, it has been used in the folk medicine (Batista, Brandão, Braga, & Oliveira, 2009). Some studies have demonstrated different activities for S. trilobata extracts, such as anti-inflammatory, antifungal, trypanosomicidal, antimicrobial, and hypoglycemic (Chiari, et al 1996;Block, Scheidt, Quintão, Santos, & Cechinel-Filho, 1998;Bresciani, et al 2004).…”

In vitro antimicrobial activity of two dibutyltin(IV) complexes of kaurenic acids derivatives

“…These compounds show wide spectral biological activity, and have been used in traditional medicine, for example, ent-kaurenic acid has been used as anti-microbial, anti-inflammatory (Sosa-Sequera et al, 2010;Cavalcanti et al, 2006), anti-fungal (Boeck et al, 2005), anti-parasitical (Diamantino et al, 2008) and others. While grandiflorenic acid has been reported with diuretic effect (Somova et al, 2001), uterotonic properties (Villa-Ruano et al, 2013) and anti-trypanosomiasis (Batista et al, 2009). Occasionally, cattle consume different species of Espeletia and other paramo vegetation, although of poor quality (Molinillo and Monasterio, 2002).…”