Influência do pneumoperitônio nas funções hepática e renal e na liberação de citocinas pró-inflamatórias em modelo experimental de endotoxemia induzida por lipopolissacarídeo de Escherichia coli

Macri, Matheus Martin; Matias, Jorge Eduardo Fouto; Souza, Carlos José Franco de; Nicoluzzi, João Eduardo Leal; Caron, Pedro Ernesto; Repka, João Carlos Domingues

doi:10.1590/s0102-67202009000400005

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…The elevation of alanine aminotransferase and aspartate aminotransferase is relatively common in sepsis and may be the result of ischemic liver injury or after re-perfusion, drug toxicity, systemic inflammation or direct action of pathogenic infectious agent, as a sign of cell injury hepatocytes and in some cases, mitochondrial dysfunction 16,17 . The elevation of direct bilirubin and enzymes of membrane, such as gamma glutamyltransferase and alkaline phosphatase indicate cholestasis inflammatory drug or obstruction.…”

Section: Discussionmentioning

confidence: 99%

Síndrome de disfunção de múltiplos órgãos induzida por sepse: estudo experimental em ratos

Lima¹,

Skare²,

Malafaia³

et al. 2011

ABCD, arq. bras. cir. dig.

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-Background -The leading cause of death in patients with sepsis in surgery is syndrome of multiple organ dysfunction. Thus, experimental models that simulate organic changes of sepsis in humans are required. Aim -To present two models that induce the syndrome of multiple organ dysfunction and to compare, the changes induced, by intravenous injection of lipopolysaccharide or cell 36UE of viable Escherichia coli in relation to mortality and survival, level of lipopolysaccharide, release of tumor necrosis factor alpha ; hematological, liver and kidney function. Method -The study lasted seven days and it was used on it 50 male Wistar rats divided into three groups: control, lipopolysaccharide and Escherichia coli. The experimental groups were inoculated and divided into two subgroups, with inocuation with 24 or 48 hours. On the seventh day were proceeded blood collection and histopathologic analysis of liver, kidneys and lungs. Results -There was a survival of ten animals in the control group; zero in bacteria group of 24 hours and six in 48 hours; ten of lipopolysaccharide in 24 hours and six in 48 hours. In the experimental groups, levels of endotoxin, tumor necrosis factor alpha, leukocytes, platelets, renal and liver levels were higher than the control group. There were histopathological changes in the bacterial group. Conclusion -The two models of sepsis induced multiple organ dysfunction syndrome; yet the administration 36UE endotoxin every 48 hours could be utilized in advantage over the other for not induce death in significant numbers during the period of seven days.

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Section: Discussionmentioning

confidence: 99%

Síndrome de disfunção de múltiplos órgãos induzida por sepse: estudo experimental em ratos

Lima¹,

Skare²,

Malafaia³

et al. 2011

ABCD, arq. bras. cir. dig.

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“…inflammatory response includes chemotactic function modulation of inducible nitric oxide synthase and induction of cytokines, among other. [6][7][8][9][10][11] The identification of a prognostic marker that may predict the outcome at the end of 24 to 48 hours of treatment of severe sepsis and septic shock could be very useful in order to provide a reassessment of the patient, identifying perpetuators of gravity, permitting interventions and conduct reorientation. The addition of this marker for the routine evaluation of patients with severe sepsis and septic shock treated according to the surviving sepsis campaign strategies might bring additional contribution towards reducing morbidity and mortality.…”

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confidence: 99%

Diagnostic utility of serum procalcitonin as a biomarker of sepsis and infection in post-operative patients at tertiary care centre

2020

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Background: Procalcitonin (PCT), the precursor peptide of calcitonin, a hormone involved in calcium homeostasis, is present in normal subjects in extremely low serum levels (0.1 to 0.5 ng/ml). In response to bacterial infectious stimulation serum procalcitonin rises substantially and its role in inflammatory response includes chemotactic function modulation of inducible nitric oxide synthase and induction of cytokines, among other. The objective of this study to assess serum procalcitonin as a biomarker of sepsis and infection in post op patients.Methods: The present descriptive observational study was conducted at Department of Surgery, Aarupadai Veedu Medical College and Hospital, Puducherry from September 2018 to June 2020. Total 48 patients who had undergone surgery and presented with post-surgical sepsis were included. Data entered in MS excel sheet and analysed by using SPSS 24.0 version IBM USA.Results: Out of 48 cases, majority of cases i.e. 18 (37.5%) were from 36-50 years age group followed by 11 (22.9%) from 51-60 years age group. Prevalence of sepsis was 20.8%, septic shock 25%, severe sepsis 33.3% and SIRS 14.6%. Procalcitonin (PCT) assay shows positive in 47 (97.9%) cases. E. coli were isolated from 5 (45.5%) cases with PCT 2-10 as compared to 6 (16.7%) cases with PCT >10.Conclusions: PCT shares remarkable promise as a marker of sepsis and can be used as a routine lab parameter. It was also found to be an accurate diagnostic parameter for differentiating various stages of sepsis and predicting the outcome.

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Procalcitonina como biomarcador de prognóstico da sepse grave e choque séptico

Azevedo¹,

Torres²,

Czeczko

et al. 2012

Rev. Col. Bras. Cir.

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OBJETIVO: Avaliar a tendência da concentração plasmática e do clearance de procalcitonina (PCT-c) como biomarcadores de prognóstico de pacientes com sepse grave e choque séptico, comparado a um outro marcador precoce de prognóstico representado pelo número de critérios de SIRS no momento do diagnóstico da sepse. MÉTODOS: Estudo de coorte prospectivo observacional onde foram incluídos pacientes com sepse grave e choque séptico. A concentração sérica de procalcitonina foi determinada no momento do diagnóstico da sepse e após 24 e 48 horas. Foram coletados dados demográficos, escore APACHE IV, escore SOFA na chegada, número de critérios de SIRS no momento do diagnóstico, sitio da infecção e resultados microbiológicos. RESULTADOS: Vinte e oito pacientes foram incluídos, 19 clínicos e nove cirúrgicos. Em 13 (46,4%) a fonte da sepse foi pulmonar, em sete abdominal (25,0%), em cinco urinária (17,9%) e de partes moles em três casos (10,7%). Quinze pacientes tinham sepse grave e 13 choque séptico. A mortalidade global foi cinco pacientes (17,9%), três deles com choque séptico. Vinte e oito determinações de PCT foram realizadas no momento do diagnóstico da sepse, 27 após 24 horas e 26 após 48 horas. A concentração inicial não se mostrou expressivamente diferente entre os grupos sobreviventes e não sobreviventes, mas as diferenças entre os dois grupos após 24 e 48 horas alcançaram significância estatística expressiva. Não se observou diferença em relação ao número de critérios de SIRS. O clearance de procalcitonina de 24 horas mostrou-se expressivamente mais elevado no grupo de sobreviventes (-3,0 versus -300,0, p=0,028). Embora o clearance de procalcitonina de 48 horas tenha mostrado resultado mais elevado no grupo de sobreviventes comparado aos não sobreviventes, a diferença não alcançou significância estatística. CONCLUSÃO: Concentrações persistentemente elevadas de procalcitonina no plasma, assim como, redução do PCT-c 24 horas, associaram-se à elevação expressiva da mortalidade de pacientes com sepse grave e choque séptico.

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Influência do pneumoperitônio nas funções hepática e renal e na liberação de citocinas pró-inflamatórias em modelo experimental de endotoxemia induzida por lipopolissacarídeo de Escherichia coli

Cited by 3 publications

References 23 publications

Síndrome de disfunção de múltiplos órgãos induzida por sepse: estudo experimental em ratos

Síndrome de disfunção de múltiplos órgãos induzida por sepse: estudo experimental em ratos

Diagnostic utility of serum procalcitonin as a biomarker of sepsis and infection in post-operative patients at tertiary care centre

Procalcitonina como biomarcador de prognóstico da sepse grave e choque séptico

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